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Q9ZCU4

- SYI_RICPR

UniProt

Q9ZCU4 - SYI_RICPR

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RP617
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10861086Isoleucine--tRNA ligasePRO_0000098560Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272947.RP617.

Structurei

3D structure databases

ProteinModelPortaliQ9ZCU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi624 – 6285"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZCU4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNTKYYPDV SANVDFAAIE QEILKFWQNN NIFQKSIDYR NGESEFIFYD
60 70 80 90 100
GPPFANGLPH YGHLLTGFIK DVYARYKTIK GKKVERRFGW DCHGLPAEMQ
110 120 130 140 150
SEKELGISGR IAITNFGIEK FNNHCRASVM QYASEWEQYV TRQARWVAFK
160 170 180 190 200
NAYKTMDKNF MESVLWAFKE LYNKDLLYES MRVMPYSWAC ETPLSNFETR
210 220 230 240 250
LDNAYRERTD KAITVSFVLN EVTLINGIIS QKSDMKEGDN FKEYRILAWT
260 270 280 290 300
TTPWTLPANL ALAVGSDIDY AFVDKNEVCY IIAASSVAKY AKELGLSGKE
310 320 330 340 350
NFEIIKGLKL QGLSYKPLFN YFENHPNSFK IFASDFVVEG DGTGIVHMAP
360 370 380 390 400
GFGEDDQILC ESKGIELVCP VDNSGKFTKE IPDLEGVQVF DANDKIIIKL
410 420 430 440 450
KEQGNWIKTE QYIHNYPHCW RTDTPLIYKA VPSWYVRVTK FKDRMVELNQ
460 470 480 490 500
QINWIPHNVK DNLFGKWLEN ARDWSISRNR FWGTPLPVWK SDDPKYPRID
510 520 530 540 550
VYGSIEEIEK DFGVKINDLH RPFIDELTRT NPDDPTGKST MRRIDDVFDC
560 570 580 590 600
WFESGSMPYG QVHYPFENKK WFVEHFPADF IVEYSSQTRG WFYTLMVLST
610 620 630 640 650
ALFDRPPFLN CICHGVILDA TGQKLSKRLN NYADPLELFD KYGSDALRVT
660 670 680 690 700
MLSSNVVKGQ ELLIDKDGKM VFDTLRLFIK PIWNAYHFFT IYANADSLKG
710 720 730 740 750
TLNFASQNVL DVYILSKLKI AVNKIEESLD NFDTQTAYHA VSEFFEVLNN
760 770 780 790 800
WYIRRSRARF WKNEKDTDKQ NAYNTLYSCL KIMTIAMSAL IPMISETIYQ
810 820 830 840 850
GLHNTAITQL NCLLSEGKHI VQNPMSDTQD YNTSVHLCNY PTLSDFEINY
860 870 880 890 900
ELVSTMDNVL DICSNSLFIR STENIRVRQP LACITIISKH NNNLKDFEDL
910 920 930 940 950
IKDEINVKTV IYRDDLENYA RKKLSLNFAI LGKRLPHKMK AIIDAAKKGE
960 970 980 990 1000
WEATTLGLAI CGEILNSDEY TLILEPYSHI KGTANFDNNS SLLILNLELT
1010 1020 1030 1040 1050
SELIEEGYAR DIVRFIQYAR KEADFSITDR ILIEIISEFD LSKIIDHYGD
1060 1070 1080
FIKEQTLGEF AKNFTPDYVS KVALENNQIQ LKVKRL
Length:1,086
Mass (Da):125,637
Last modified:May 1, 1999 - v1
Checksum:iDEC857A0D81E5963
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235272 Genomic DNA. Translation: CAA15060.1.
PIRiB71667.
RefSeqiNP_220984.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15060; CAA15060; CAA15060.
GeneIDi883643.
KEGGirpr:RP617.
PATRICi17902053. VBIRicPro72556_0636.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235272 Genomic DNA. Translation: CAA15060.1 .
PIRi B71667.
RefSeqi NP_220984.1. NC_000963.1.

3D structure databases

ProteinModelPortali Q9ZCU4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272947.RP617.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA15060 ; CAA15060 ; CAA15060 .
GeneIDi 883643.
KEGGi rpr:RP617.
PATRICi 17902053. VBIRicPro72556_0636.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.

Entry informationi

Entry nameiSYI_RICPR
AccessioniPrimary (citable) accession number: Q9ZCU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3