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Q9ZCU4

- SYI_RICPR

UniProt

Q9ZCU4 - SYI_RICPR

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Protein

Isoleucine--tRNA ligase

Gene
ileS, RP617
Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:RP617
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10861086Isoleucine--tRNA ligaseUniRule annotationPRO_0000098560Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272947.RP617.

Structurei

3D structure databases

ProteinModelPortaliQ9ZCU4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionUniRule annotationAdd
BLAST
Motifi624 – 6285"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZCU4-1 [UniParc]FASTAAdd to Basket

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MTNTKYYPDV SANVDFAAIE QEILKFWQNN NIFQKSIDYR NGESEFIFYD     50
GPPFANGLPH YGHLLTGFIK DVYARYKTIK GKKVERRFGW DCHGLPAEMQ 100
SEKELGISGR IAITNFGIEK FNNHCRASVM QYASEWEQYV TRQARWVAFK 150
NAYKTMDKNF MESVLWAFKE LYNKDLLYES MRVMPYSWAC ETPLSNFETR 200
LDNAYRERTD KAITVSFVLN EVTLINGIIS QKSDMKEGDN FKEYRILAWT 250
TTPWTLPANL ALAVGSDIDY AFVDKNEVCY IIAASSVAKY AKELGLSGKE 300
NFEIIKGLKL QGLSYKPLFN YFENHPNSFK IFASDFVVEG DGTGIVHMAP 350
GFGEDDQILC ESKGIELVCP VDNSGKFTKE IPDLEGVQVF DANDKIIIKL 400
KEQGNWIKTE QYIHNYPHCW RTDTPLIYKA VPSWYVRVTK FKDRMVELNQ 450
QINWIPHNVK DNLFGKWLEN ARDWSISRNR FWGTPLPVWK SDDPKYPRID 500
VYGSIEEIEK DFGVKINDLH RPFIDELTRT NPDDPTGKST MRRIDDVFDC 550
WFESGSMPYG QVHYPFENKK WFVEHFPADF IVEYSSQTRG WFYTLMVLST 600
ALFDRPPFLN CICHGVILDA TGQKLSKRLN NYADPLELFD KYGSDALRVT 650
MLSSNVVKGQ ELLIDKDGKM VFDTLRLFIK PIWNAYHFFT IYANADSLKG 700
TLNFASQNVL DVYILSKLKI AVNKIEESLD NFDTQTAYHA VSEFFEVLNN 750
WYIRRSRARF WKNEKDTDKQ NAYNTLYSCL KIMTIAMSAL IPMISETIYQ 800
GLHNTAITQL NCLLSEGKHI VQNPMSDTQD YNTSVHLCNY PTLSDFEINY 850
ELVSTMDNVL DICSNSLFIR STENIRVRQP LACITIISKH NNNLKDFEDL 900
IKDEINVKTV IYRDDLENYA RKKLSLNFAI LGKRLPHKMK AIIDAAKKGE 950
WEATTLGLAI CGEILNSDEY TLILEPYSHI KGTANFDNNS SLLILNLELT 1000
SELIEEGYAR DIVRFIQYAR KEADFSITDR ILIEIISEFD LSKIIDHYGD 1050
FIKEQTLGEF AKNFTPDYVS KVALENNQIQ LKVKRL 1086
Length:1,086
Mass (Da):125,637
Last modified:May 1, 1999 - v1
Checksum:iDEC857A0D81E5963
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ235272 Genomic DNA. Translation: CAA15060.1.
PIRiB71667.
RefSeqiNP_220984.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15060; CAA15060; CAA15060.
GeneIDi883643.
KEGGirpr:RP617.
PATRICi17902053. VBIRicPro72556_0636.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ235272 Genomic DNA. Translation: CAA15060.1 .
PIRi B71667.
RefSeqi NP_220984.1. NC_000963.1.

3D structure databases

ProteinModelPortali Q9ZCU4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272947.RP617.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA15060 ; CAA15060 ; CAA15060 .
GeneIDi 883643.
KEGGi rpr:RP617.
PATRICi 17902053. VBIRicPro72556_0636.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.

Entry informationi

Entry nameiSYI_RICPR
AccessioniPrimary (citable) accession number: Q9ZCU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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