Q9ZCT8 (SYE2_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	RP623

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Glutamate--tRNA ligase 2 HAMAP-Rule MF_00022		PRO_0000119641
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	239 – 243	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCT8 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: DF1CE50A20B8A9FD	FASTA	470	53,697
	10 20 30 40 50 60 MTNIITRFAP SPTGFLHIGS ARTALFNYLF ARHNNGKFFL RIEDTDKKRS TKEAVEAIFS 70 80 90 100 110 120 GLKWLGLNWD GEVIFQSKRN SLYKEAALKL LKEGKAYYCF TRQEEIAKQR QQALKDKQHF 130 140 150 160 170 180 IFNSEWRDKG PSTYPADIKP VIRLKVPREG SITIHDTLQG EIVIENSHID DMILIRTDGT 190 200 210 220 230 240 ATYMLAVIVD DHDMGITHII RGDDHLTNAA RQIAIYHAFG YEVPNMTHIP LIHGADGTKL 250 260 270 280 290 300 SKRHGALGVE AYKDMGYLPE SLCNYLLRLG WSHGDDEIIS MNQAIEWFNL ASLGKSPSKL 310 320 330 340 350 360 DFAKMNSINS HYLRMLDNDS LTSKTVEILK QNYKISEKEV SYIKQAMPSL IVRSETLRDL 370 380 390 400 410 420 AQLAYIYLVD SPMIYSQDAK EVINNCDKDL IKQVIENLSK LEQFNKECVQ NKFKEIAIYN 430 440 450 460 470 GLKLNDIMKP VRALITGMTA SPSVFEIAET LGKENILKRL KIIYYNNLNF

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235272 Genomic DNA. Translation: CAA15066.1.
PIR	H71667.
RefSeq	NP_220990.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZCT8.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP623.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA15066; CAA15066; CAA15066.
GeneID	883788.
KEGG	rpr:RP623.
PATRIC	17902067. VBIRicPro72556_0643.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
OMA	WENVRVR.
ProtClustDB	PRK01406.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_RICPR

Accession

Primary (citable) accession number: Q9ZCT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program