Q9ZCS9 (RPOA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit alpha
Short name=RNAP subunit alpha
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit alpha
Transcriptase subunit alpha

Gene names

Name:	rpoA
Ordered Locus Names:	RP635

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00059
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00059
Subunit structure	Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Domain	The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements By similarity. HAMAP MF_00059
Sequence similarities	Belongs to the RNA polymerase alpha chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	DNA-directed RNA polymerase subunit alpha HAMAP MF_00059		PRO_0000175369
Regions
	Region	1 – 236	236	Alpha N-terminal domain (alpha-NTD) By similarity
	Region	251 – 340	90	Alpha C-terminal domain (alpha-CTD) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCS9 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 754F5542943ED254	FASTA	340	38,360
	10 20 30 40 50 60 MLSLSKNWNT LIKPNRVTYE NFPETNNKAK IIVEPLERGF GLTLGNAMRR VLLSSLQGAA 70 80 90 100 110 120 ITSIKIPAIE HEFSSIPGVQ EDVSEVILNI KGIEVKMHVS EKRIIKLKAM GPCVVTAGMI 130 140 150 160 170 180 DTGHDVEILN PDHVICNLAK NKQLEMELTC KVGKGYVLST NSYEDNLPIG EIAIDALFNP 190 200 210 220 230 240 VKSVTYKVEN TRVGQVTDYD KLIMFVETNG DVLPEMAVGL AARILQEQLQ LFIAFEEQEE 250 260 270 280 290 300 DKQVKTDSLP FSPYLLKRVD ELELSVRSAN CLKNDNIIYI GDLVKRTESD MLRTPNFGRK 310 320 330 340 SLNEIKEILA KFNLRFGMDV PDWPPENIQE LSKRYEDSYN

References

[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235272 Genomic DNA. Translation: CAA15075.1.
PIR	A71669.
RefSeq	NP_220999.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZCS9.
SMR	Q9ZCS9. Positions 253-323.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883988.
GenomeReviews	Gene locus RP635 in contig AJ235269_GR.
KEGG	rpr:RP635.
PATRIC	17902097. VBIRicPro72556_0657.
Phylogenomic databases
HOGENOM	HBG430844.
OMA	VRSHNCL.
ProtClustDB	PRK05182.
Enzyme and pathway databases
BioCyc	RPRO272947:RP635-MONOMER.
Family and domain databases
HAMAP	MF_00059. RNApol_bact_RpoA. [Tree]
InterPro	IPR011261. DNA-dir_RNA_pol_dimersation. IPR011262. DNA-dir_RNA_pol_insert. IPR009025. DNA-dir_RNA_pol_RBP11-like. IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3. IPR011773. DNA-dir_RpoA. IPR011260. RNAP_asu_C. [Graphical view]
Gene3D	G3DSA:2.170.120.12. RNAP_insert. 1 hit.
KO	K03040.
Pfam	PF01000. RNA_pol_A_bac. 1 hit. PF03118. RNA_pol_A_CTD. 1 hit. PF01193. RNA_pol_L. 1 hit. [Graphical view]
ProDom	PD001179. RNAP_asu_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00662. RPOLD. 1 hit. [Graphical view]
SUPFAM	SSF47789. RNAP_alpha_C. 1 hit. SSF56553. RNAP_insert. 1 hit. SSF55257. RNAP_RBP11-like. 1 hit.
TIGRFAMs	TIGR02027. RpoA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOA_RICPR

Accession

Primary (citable) accession number: Q9ZCS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program