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Q9ZCQ4

- FUMC_RICPR

UniProt

Q9ZCQ4 - FUMC_RICPR

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Protein

Fumarate hydratase class II

Gene
fumC, RP665
Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible addition of water to fumarate to give L-malate By similarity.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881Proton donor/acceptor By similarity
Active sitei318 – 3181 By similarity
Binding sitei319 – 3191Substrate
Sitei331 – 3311Important for catalytic activity By similarity

GO - Molecular functioni

  1. fumarate hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fumarate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class II (EC:4.2.1.2)
Short name:
Fumarase C
Gene namesi
Name:fumC
Ordered Locus Names:RP665
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. tricarboxylic acid cycle enzyme complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Fumarate hydratase class IIUniRule annotationPRO_0000161307Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi272947.RP665.

Structurei

Secondary structure

1
461
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96
Beta strandi12 – 176
Helixi24 – 329
Helixi42 – 6120
Helixi67 – 8216
Turni83 – 875
Beta strandi92 – 954
Helixi100 – 11920
Beta strandi124 – 1285
Helixi130 – 1345
Turni135 – 1373
Helixi140 – 15819
Helixi160 – 17516
Helixi178 – 1803
Beta strandi182 – 1876
Beta strandi190 – 1967
Helixi197 – 21923
Turni220 – 2234
Turni230 – 2323
Helixi242 – 25413
Helixi264 – 2696
Helixi272 – 29827
Beta strandi302 – 3054
Beta strandi319 – 3213
Helixi328 – 35124
Helixi362 – 38625
Turni387 – 3904
Helixi395 – 40410
Helixi406 – 4083
Helixi409 – 4168
Helixi418 – 43114
Helixi435 – 4417
Helixi447 – 4548

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GTDX-ray2.40A/B1-461[»]
ProteinModelPortaliQ9ZCQ4.
SMRiQ9ZCQ4. Positions 4-460.

Miscellaneous databases

EvolutionaryTraceiQ9ZCQ4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 1003Substrate bindingUniRule annotation
Regioni129 – 1324B site By similarity
Regioni139 – 1413Substrate binding Inferred
Regioni187 – 1882Substrate binding By similarity
Regioni324 – 3263Substrate bindingUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0114.
HOGENOMiHOG000061736.
KOiK01679.
OMAiMESFNIH.
OrthoDBiEOG6V1M4M.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC.
InterProiIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZCQ4-1 [UniParc]FASTAAdd to Basket

« Hide

MKNYRIESDS FGEIQIEEKF YWGAQTQRSL NNFKISKQKM PKILIRALAI    50
LKKCAAQVNY EFGDLEYKIA TSIDKAIDRI LAGEFEDNFP LVVWQTGSGT 100
QTNMNMNEVI ASIANEELTG KKGGKFPVHP NDHVNKGQSS NDSFPTAMHI 150
ATVLATKQQL IPALNNLLTY LQDKSKDWDK IIKIGRTHLQ DATPLTLKQE 200
FSGYITQIEY ALERIEDALK KVYLLAQGGT AVGTGINSKI GFDIKFAQKV 250
AEFTQQPFKT APNKFESLAA HDALVEFSGT LNTIAVSLMK IANDIRLLGS 300
GPRCGLGELH LPENEPGSSI MPGKVNPTQV EALTMVCTQV MGNHVTVTIA 350
GSNGHLELNV FKPVIIYNIL QSIELLSDSV NSFVTHCVKG LEPNIARINT 400
LRDKSLMLVT VLNPHIGYDN AAKIAKEAHK YGITLKEAAK KLNFLSEEEF 450
DKIVVPEKMI S 461
Length:461
Mass (Da):51,008
Last modified:May 1, 1999 - v1
Checksum:iC9929CC94E7E617D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ235272 Genomic DNA. Translation: CAA15103.1.
PIRiE71672.
RefSeqiNP_221027.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15103; CAA15103; CAA15103.
GeneIDi883633.
KEGGirpr:RP665.
PATRICi17902157. VBIRicPro72556_0685.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ235272 Genomic DNA. Translation: CAA15103.1 .
PIRi E71672.
RefSeqi NP_221027.1. NC_000963.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3GTD X-ray 2.40 A/B 1-461 [» ]
ProteinModelPortali Q9ZCQ4.
SMRi Q9ZCQ4. Positions 4-460.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272947.RP665.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA15103 ; CAA15103 ; CAA15103 .
GeneIDi 883633.
KEGGi rpr:RP665.
PATRICi 17902157. VBIRicPro72556_0685.

Phylogenomic databases

eggNOGi COG0114.
HOGENOMi HOG000061736.
KOi K01679.
OMAi MESFNIH.
OrthoDBi EOG6V1M4M.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01007 .

Miscellaneous databases

EvolutionaryTracei Q9ZCQ4.

Family and domain databases

Gene3Di 1.10.275.10. 1 hit.
HAMAPi MF_00743. FumaraseC.
InterProi IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view ]
PANTHERi PTHR11444. PTHR11444. 1 hit.
Pfami PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view ]
PRINTSi PR00149. FUMRATELYASE.
SUPFAMi SSF48557. SSF48557. 1 hit.
TIGRFAMsi TIGR00979. fumC_II. 1 hit.
PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.
  2. "Structure of fumarate hydratase from Rickettsia prowazekii, the agent of typhus and suspected relative of the mitochondria."
    Phan I., Subramanian S., Olsen C., Edwards T.E., Guo W., Zhang Y., Van Voorhis W.C., Stewart L.J., Myler P.J.
    Acta Crystallogr. F 67:1123-1128(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS, SUBUNIT.

Entry informationi

Entry nameiFUMC_RICPR
AccessioniPrimary (citable) accession number: Q9ZCQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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