Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q9ZCI1 (MNME_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA modification GTPase MnmE
EC=3.6.-.-
Gene names
Name:mnmE
Synonyms:thdF, trmE
Ordered Locus Names:RP759
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group ›

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00379

Cofactor

Binds 1 potassium ion per subunit By similarity.

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily.

Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: HAMAP

GTPase activity

Inferred from electronic annotation. Source: HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445tRNA modification GTPase MnmE HAMAP-Rule MF_00379
PRO_0000188910

Regions

Domain227 – 330104G
Nucleotide binding225 – 2306GTP By similarity
Nucleotide binding244 – 2507GTP By similarity
Nucleotide binding269 – 2724GTP By similarity

Sites

Metal binding2251Potassium By similarity
Metal binding2291Magnesium By similarity
Metal binding2441Potassium; via carbonyl oxygen By similarity
Metal binding2461Potassium; via carbonyl oxygen By similarity
Metal binding2491Potassium By similarity
Metal binding2501Magnesium By similarity
Binding site201Formyltetrahydrofolate By similarity
Binding site791Formyltetrahydrofolate By similarity
Binding site1191Formyltetrahydrofolate By similarity
Binding site4451Formyltetrahydrofolate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZCI1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: FA158F99E716B4F6

FASTA44549,813
        10         20         30         40         50         60 
METIFAQSSA FGKAGVAVFR ISGPKSLEVL QLLTGRKDFK PRLMYYQQII VPETNELIDN 

        70         80         90        100        110        120 
AMVVYFKLPN SFTGEDVVEI HTHGSKAISI MLINTLLNIA DIRLAEAGEF TKRAFLNNKF 

       130        140        150        160        170        180 
DLTAAEGIAD LINAETIMQH RQAVRQANGG LKELYNKWRN QLLKIISLLE AYIDFPDEDI 

       190        200        210        220        230        240 
PESILNDVNN THKNIVNEIS NYLNDNRRGE LLNNGLKLAI IGPPNTGKSS LLNFLMQRNI 

       250        260        270        280        290        300 
AIVSNIAGTT RDIIEGHLDI GGYPIILQDT AGIRTESTDI IEQEGIKRAI NSAKTANIKI 

       310        320        330        340        350        360 
VMFDAEKLDS SINNDITGLI DENTIVIINK IDLIEPNKTF AIENRYKCLR VSVKNNIALS 

       370        380        390        400        410        420 
NILKNIENIA ENLAGVTETP YITNQRHRHY LKQALSHLID FNLDNDLVLA TEDIRMTVRC 

       430        440 
IGLITGVINV EEILNEIFKN FCIGK

« Hide

References

[1]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ235273 Genomic DNA. Translation: CAA15187.1.
PIRC71636.
RefSeqNP_221111.1. NC_000963.1.

3D structure databases

ProteinModelPortalQ9ZCI1.
ModBaseSearch...

Protein-protein interaction databases

STRING272947.RP759.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA15187; CAA15187; CAA15187.
GeneID883741.
KEGGrpr:RP759.
PATRIC17902383. VBIRicPro72556_0795.

Phylogenomic databases

eggNOGCOG0486.
HOGENOMHOG000200714.
KOK03650.
OMAPGEFSEQ.
ProtClustDBPRK05291.

Family and domain databases

Gene3D3.30.1360.120. 1 hit.
HAMAPMF_00379. GTPase_MnmE.
InterProIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamPF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNME_RICPR
AccessionPrimary (citable) accession number: Q9ZCI1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents