Q9ZCG1 (NUOL_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit L
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit L
NDH-1 subunit L

Gene names

Name:	nuoL
Ordered Locus Names:	RP792

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 5 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 653	653	NADH-quinone oxidoreductase subunit L		PRO_0000118223
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	89 – 109	21	Helical; Potential
	Transmembrane	115 – 135	21	Helical; Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Transmembrane	178 – 198	21	Helical; Potential
	Transmembrane	214 – 234	21	Helical; Potential
	Transmembrane	253 – 273	21	Helical; Potential
	Transmembrane	285 – 305	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Transmembrane	336 – 356	21	Helical; Potential
	Transmembrane	377 – 397	21	Helical; Potential
	Transmembrane	418 – 438	21	Helical; Potential
	Transmembrane	465 – 485	21	Helical; Potential
	Transmembrane	511 – 531	21	Helical; Potential
	Transmembrane	577 – 597	21	Helical; Potential
	Transmembrane	629 – 649	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCG1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 540436E913D097B4	FASTA	653	73,478
	10 20 30 40 50 60 MIHQNLAIMI IILPLVSSII NGLFLNIIDK KLAKIIAISF LSLSALFSLV IFCDVTLVGK 70 80 90 100 110 120 IIHIKLLPWI EFKNLQVNWS IYIDQLTSIM FIAVTFVSSV VHIYSLGYMA EDKGIIRFLS 130 140 150 160 170 180 FLSLFTFFML MLVSADNFLQ LFCGWEGVGV CSYLLIGFWH SKESANKAAI KAFITNRVSD 190 200 210 220 230 240 FAFILGIITI IVYYGSANYK DVFSSAKLLS NTKIFVHFSI LDIICLLLFI GCMGKSAQIG 250 260 270 280 290 300 LHVWLPDAME GPTPVSALIH AATMVTAGVF LVARCSYLFE YSPIVLQFIT IIGGITCLFA 310 320 330 340 350 360 ASIAIMQSDI KKIIAYSTCS QLGYMFMACG VSSYNSAIFH LVTHAFFKAL LFLSAGNVIH 370 380 390 400 410 420 AVNEHNIFKM GGLINKMPIT YGNFLIGSLA LIGIYPLSGF YSKDLILEAT YSSGSFMFIF 430 440 450 460 470 480 GIITAILTAI YSMKIIILVF HGKTKLEKDV FKHAHEPTKI MNNPLILLVA GSFFSGMIGY 490 500 510 520 530 540 YLLSMDKPNG YFHESLFNLH IYKLLINHHP LYIKLLPMAV GIVGIIIGIC LYKGSLSYQT 550 560 570 580 590 600 LTNESDQREK DWIPKSKCKM ILVFISNVLR NKYYFDEIYN HLIIKPIHCL TYLFYFGDQK 610 620 630 640 650 IIDRFGPNGF ARVINYFCAV TCKIQTGYIF NYTLYIVSFI VVTISYFVLK NIY

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235273 Genomic DNA. Translation: CAA15218.1.
PIR	B71640.
RefSeq	NP_221142.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZCG1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883813.
GenomeReviews	Gene locus RP792 in contig AJ235269_GR.
KEGG	rpr:RP792.
PATRIC	17902452. VBIRicPro72556_0828.
Phylogenomic databases
HOGENOM	HBG643593.
OMA	KLLPMAV.
ProtClustDB	PRK06590.
Enzyme and pathway databases
BioCyc	RPRO272947:RP792-MONOMER.
Family and domain databases
InterPro	IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR001516. NADH_UbQ_OxRdtase_chain5/L_N. IPR003945. NADHpl_OxRdtase_5. IPR018393. NADHpl_OxRdtase_5_subgr. [Graphical view]
KO	K00341.
Pfam	PF00361. Oxidored_q1. 1 hit. PF00662. Oxidored_q1_N. 1 hit. [Graphical view]
PRINTS	PR01435. NPOXDRDTASE5.
TIGRFAMs	TIGR01974. NDH_I_L. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NUOL_RICPR

Accession

Primary (citable) accession number: Q9ZCG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program