Q9ZCG0 (NUOM_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit M
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit M
NDH-1 subunit M

Gene names

Name:	nuoM
Ordered Locus Names:	RP793

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 4 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	NADH-quinone oxidoreductase subunit M		PRO_0000118047
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	87 – 107	21	Helical; Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Transmembrane	137 – 157	21	Helical; Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Transmembrane	203 – 223	21	Helical; Potential
	Transmembrane	249 – 269	21	Helical; Potential
	Transmembrane	278 – 298	21	Helical; Potential
	Transmembrane	315 – 335	21	Helical; Potential
	Transmembrane	336 – 356	21	Helical; Potential
	Transmembrane	373 – 393	21	Helical; Potential
	Transmembrane	409 – 429	21	Helical; Potential
	Transmembrane	456 – 476	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCG0 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 55C38A584CA74FF3	FASTA	491	55,260
	10 20 30 40 50 60 MLELPIISIT IFLPLISVLY ILLFFNQNKK ADKLSIYVAM LSSVLTFIST IYILIEFDVS 70 80 90 100 110 120 NNTYQFVERY TWLDKIGLEF HVGVDGIAIF FVVLTSFLTL ICIIGSLFTI KKYIKEYLVC 130 140 150 160 170 180 FLLMESLCIG AFTSINLLLF YLFFEAILVP MYIIIGVWGG DNRIYAALKF FLYTFFGSVF 190 200 210 220 230 240 FLLALIYIYS KIHSFDLTNI LELIGNIPLF AQKILWWAIF IAFAIKTPMI PFHTWLPDAH 250 260 270 280 290 300 VQAPTTGSVI LAGILLKLGG YGFLRVLLPL FPNASQEFAI YVIYLSVIAI IYASLVALAQ 310 320 330 340 350 360 KDIKQMIAYS SIAHMGYVTI GIFSFTEIGI SGAIFQMLSH GIISSSLFLI VGTLYERLHT 370 380 390 400 410 420 KEIAKYGGVA NKMPILATFF MIAMLSSIGL PSTSGFIGEF LSLLGIYKVN VVTAFIAALG 430 440 450 460 470 480 IILGAVYMLK LYKEVMLGEI TNTEIKHFRD LYKYEIISIA PLILLIIYFG LMPNSILNVF 490 HLSVENLLIK F

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235273 Genomic DNA. Translation: CAA15219.1.
PIR	C71640.
RefSeq	NP_221143.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZCG0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883536.
GenomeReviews	Gene locus RP793 in contig AJ235269_GR.
KEGG	rpr:RP793.
PATRIC	17902454. VBIRicPro72556_0829.
Phylogenomic databases
HOGENOM	HBG643593.
OMA	WINTTSH.
ProtClustDB	PRK05846.
Enzyme and pathway databases
BioCyc	RPRO272947:RP793-MONOMER.
Family and domain databases
InterPro	IPR010227. NADH_Q_OxRdtase_chainM/4. IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003918. NADH_UbQ_OxRdtase. [Graphical view]
KO	K00342.
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
PRINTS	PR01437. NUOXDRDTASE4.
TIGRFAMs	TIGR01972. NDH_I_M. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NUOM_RICPR

Accession

Primary (citable) accession number: Q9ZCG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program