Q9ZCF4 (ACON_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aconitate hydratase
Short name=Aconitase
EC=4.2.1.3

Alternative name(s):
Citrate hydro-lyase

Gene names

Name:	acnA
Ordered Locus Names:	RP799

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity.
Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC citrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC isocitrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Aconitate hydratase		PRO_0000076665
Sites
	Metal binding	426	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	492	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	495	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCF4 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2835539115E66805	FASTA	878	97,120
	10 20 30 40 50 60 MSKVHNSEYI KELVVDNTSY KIYDINKAAS DIGLPLKKLP YSLRVLLENV LRTNGNKENL 70 80 90 100 110 120 LVFKEWLKTK KSNTEIDFMP ARVLMQDFTG VPAIVDLAAM RDAMQKIGCN PLKINPLIPV 130 140 150 160 170 180 DLVIDHSVSV DSYGNKESFD QNVHMEMKRN IERYQFLKWG QQAFNNFKVV PPGTGICHQV 190 200 210 220 230 240 NLEFLSQVVW HNDGTAYPDS LVGTDSHTTM VNGLSVLGWG VGGIEAESAM LGQPITMIIP 250 260 270 280 290 300 EVIGVKLIGK LAGMATATDL VLTITEILRR KKVVGKFVEF FGNGLRNLTI SDRATISNMS 310 320 330 340 350 360 PEYGATCGFF PIDQETLKYL EVTGREITQI KLVEKYAIEQ NLWYNCEDTQ EYTEVLELDL 370 380 390 400 410 420 STVYSSLAGP KRPQDRVNLN CVASNFQNEL PYFALANKDI DKKYAVANQN YAIGNGDVVI 430 440 450 460 470 480 AAITSCTNTS NPSVMIGAAL LAKKALEHGL NVKPWVKTSL APGSKVVTEY LKLSGLDKYL 490 500 510 520 530 540 DALGFNLVGY GCTTCIGNSG SLNPEIENTI NKNRLVVASV LSGNRNFEGR INPLTKASYL 550 560 570 580 590 600 ASPILVVAYA LSGTLNIDLT NTPIGANIYL KDIWPSQKEI DEVIANSINS SMFIEKYADV 610 620 630 640 650 660 FNGTKEWRDL QITTGTNYNW DKNSTYINNP PYFDNIGSEH SIKDIKSARI LAIFGDSITT 670 680 690 700 710 720 DHISPAGSIS KNSPAAKYLI KHHIEPLDFN SYGSRRGNHE VMMRGTFANI RIKNEMCNGV 730 740 750 760 770 780 EGGFTINQLS GVQQTIYDTA MDYKAHDIPL VIFAGKEYGS GSSRDWAAKG PGLLGIKAVI 790 800 810 820 830 840 AESFERIHRS NLVGMGILPL TFTGKNTRLN LKLDGSEIID LIGLSENIKP YNLVKCVIKK 850 860 870 QTNEISTIDL ILQIFTENEI NYIKHGSIMQ FVVESLKG

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235273 Genomic DNA. Translation: CAA15225.1.
PIR	A71641.
RefSeq	NP_221149.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZCF4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883812.
GenomeReviews	Gene locus RP799 in contig AJ235269_GR.
KEGG	rpr:RP799.
PATRIC	17902466. VBIRicPro72556_0835.
Phylogenomic databases
HOGENOM	HBG289738.
OMA	NADQAGE.
ProtClustDB	PRK09277.
Enzyme and pathway databases
BioCyc	RPRO272947:RP799-MONOMER.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
KO	K01681.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit. SSF53732. Aconitase_N. 1 hit.
TIGRFAMs	TIGR01341. Aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_RICPR

Accession

Primary (citable) accession number: Q9ZCF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program