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Q9ZCD3

- MAP1_RICPR

UniProt

Q9ZCD3 - MAP1_RICPR

Protein

Methionine aminopeptidase

Gene

map

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei78 – 781SubstrateUniRule annotation
    Metal bindingi95 – 951Divalent metal cation 1
    Metal bindingi106 – 1061Divalent metal cation 1
    Metal bindingi106 – 1061Divalent metal cation 2; catalytic
    Metal bindingi169 – 1691Divalent metal cation 2; catalytic; via tele nitrogen
    Binding sitei176 – 1761Substrate
    Metal bindingi202 – 2021Divalent metal cation 2; catalytic
    Binding sitei220 – 2201Substrate
    Metal bindingi234 – 2341Divalent metal cation 1
    Metal bindingi234 – 2341Divalent metal cation 2; catalytic

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-HAMAP
    2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Protein family/group databases

    MEROPSiM24.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAPUniRule annotation
    Short name:
    MetAPUniRule annotation
    Alternative name(s):
    Peptidase MUniRule annotation
    Gene namesi
    Name:mapUniRule annotation
    Ordered Locus Names:RP824
    OrganismiRickettsia prowazekii (strain Madrid E)
    Taxonomic identifieri272947 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000002480: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 259259Methionine aminopeptidasePRO_0000148950Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272947.RP824.

    Structurei

    Secondary structure

    1
    259
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 2821
    Helixi29 – 313
    Helixi38 – 5114
    Turni57 – 604
    Helixi61 – 633
    Beta strandi66 – 727
    Beta strandi75 – 773
    Beta strandi91 – 10010
    Beta strandi103 – 11210
    Helixi118 – 13619
    Helixi144 – 15714
    Beta strandi168 – 1703
    Beta strandi172 – 18110
    Beta strandi198 – 2014
    Beta strandi204 – 2085
    Beta strandi212 – 2143
    Turni216 – 2183
    Beta strandi222 – 2243
    Beta strandi230 – 2323
    Beta strandi234 – 2396
    Beta strandi241 – 2477

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3MR1X-ray2.00A/B/C/D3-249[»]
    3MX6X-ray1.70A/B3-259[»]
    ProteinModelPortaliQ9ZCD3.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9ZCD3.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0024.
    HOGENOMiHOG000030427.
    KOiK01265.
    OMAiMPGDRPL.
    OrthoDBiEOG6MWNDS.

    Family and domain databases

    Gene3Di3.90.230.10. 1 hit.
    HAMAPiMF_01974. MetAP_1.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002467. Pept_M24A_MAP1.
    [Graphical view]
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 1 hit.
    TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
    PROSITEiPS00680. MAP_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZCD3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIKIHTEKD FIKMRAAGKL AAETLDFITD HVKPNVTTNS LNDLCHNFIT    50
    SHNAIPAPLN YKGFPKSICT SINHVVCHGI PNDKPLKNGD IVNIDVTVIL 100
    DGWYGDTSRM YYVGDVAIKP KRLIQVTYDA MMKGIEVVRP GAKLGDIGYA 150
    IQSYAEKHNY SVVRDYTGHG IGRVFHDKPS ILNYGRNGTG LTLKEGMFFT 200
    VEPMINAGNY DTILSKLDGW TVTTRDKSLS AQFEHTIGVT KDGFEIFTLS 250
    PKKLDYPPY 259
    Length:259
    Mass (Da):28,861
    Last modified:May 1, 1999 - v1
    Checksum:i2CBFE88F0DD67B61
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ235273 Genomic DNA. Translation: CAA15249.1.
    PIRiA71644.
    RefSeqiNP_221173.1. NC_000963.1.

    Genome annotation databases

    EnsemblBacteriaiCAA15249; CAA15249; CAA15249.
    GeneIDi883940.
    KEGGirpr:RP824.
    PATRICi17902518. VBIRicPro72556_0860.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ235273 Genomic DNA. Translation: CAA15249.1 .
    PIRi A71644.
    RefSeqi NP_221173.1. NC_000963.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3MR1 X-ray 2.00 A/B/C/D 3-249 [» ]
    3MX6 X-ray 1.70 A/B 3-259 [» ]
    ProteinModelPortali Q9ZCD3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272947.RP824.

    Protein family/group databases

    MEROPSi M24.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA15249 ; CAA15249 ; CAA15249 .
    GeneIDi 883940.
    KEGGi rpr:RP824.
    PATRICi 17902518. VBIRicPro72556_0860.

    Phylogenomic databases

    eggNOGi COG0024.
    HOGENOMi HOG000030427.
    KOi K01265.
    OMAi MPGDRPL.
    OrthoDBi EOG6MWNDS.

    Miscellaneous databases

    EvolutionaryTracei Q9ZCD3.

    Family and domain databases

    Gene3Di 3.90.230.10. 1 hit.
    HAMAPi MF_01974. MetAP_1.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002467. Pept_M24A_MAP1.
    [Graphical view ]
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 1 hit.
    TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
    PROSITEi PS00680. MAP_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Madrid E.
    2. "Crystal structure of methionine aminopeptidase from Rickettsia prowazekii."
      Seattle structural genomics center for infectious disease (SSGCID)
      Edwards T.E., Abendroth J., Sankaran B.
      Submitted (APR-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 3-249 IN COMPLEX WITH MANGANESE IONS.
    3. "Crystal structure of methionine aminopeptidase from Rickettsia prowazekii bound to methionine."
      Seattle Structural Genomics Center for Infectious Disease (SSGCID)
      Edwards T.E., Abendroth J., Arakaki T., Sankaran B.
      Submitted (MAY-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 3-259 IN COMPLEX WITH MANGANESE IONS AND METHIONINE.

    Entry informationi

    Entry nameiMAP1_RICPR
    AccessioniPrimary (citable) accession number: Q9ZCD3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. Rickettsia prowazekii
      Rickettsia prowazekii (strain Madrid E): entries and gene names
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3