Reviewed, UniProtKB/Swiss-Prot Q9ZCD3 (AMPM_RICPR)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	RP824

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Methionine aminopeptidase		PRO_0000148950
Sites
	Metal binding	95	1	Cobalt 1 By similarity
	Metal binding	106	1	Cobalt 1 By similarity
	Metal binding	106	1	Cobalt 2 By similarity
	Metal binding	169	1	Cobalt 2 By similarity
	Metal binding	202	1	Cobalt 2 By similarity
	Metal binding	234	1	Cobalt 1 By similarity
	Metal binding	234	1	Cobalt 2 By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	176	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCD3-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2CBFE88F0DD67B61	FASTA	259	28,861
	10 20 30 40 50 60 MTIKIHTEKD FIKMRAAGKL AAETLDFITD HVKPNVTTNS LNDLCHNFIT SHNAIPAPLN 70 80 90 100 110 120 YKGFPKSICT SINHVVCHGI PNDKPLKNGD IVNIDVTVIL DGWYGDTSRM YYVGDVAIKP 130 140 150 160 170 180 KRLIQVTYDA MMKGIEVVRP GAKLGDIGYA IQSYAEKHNY SVVRDYTGHG IGRVFHDKPS 190 200 210 220 230 240 ILNYGRNGTG LTLKEGMFFT VEPMINAGNY DTILSKLDGW TVTTRDKSLS AQFEHTIGVT 250 KDGFEIFTLS PKKLDYPPY

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235273 Genomic DNA. Translation: CAA15249.1.
PIR	A71644.
RefSeq	NP_221173.1.
3D structure databases
HSSP	HSSP built from PDB template 1MAT based on UniProtKB P07906.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Genome annotation databases
GeneID	883940.
GenomeReviews	Gene locus RP824 in contig AJ235269_GR.
KEGG	rpr:RP824.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZCD3.
OMA	TTNSLND.
Enzyme and pathway databases
BioCyc	RPRO272947:RP824-MON.
BRENDA	3.4.11.18. 141977.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_RICPR

Accession

Primary (citable) accession number: Q9ZCD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)