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Protein

Methionine aminopeptidase

Gene

map

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation2 Publications, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78SubstrateUniRule annotation1
Metal bindingi95Divalent metal cation 1UniRule annotation2 Publications1
Metal bindingi106Divalent metal cation 1UniRule annotation2 Publications1
Metal bindingi106Divalent metal cation 2; catalyticUniRule annotation2 Publications1
Metal bindingi169Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation2 Publications1
Binding sitei176SubstrateUniRule annotation1 Publication1
Metal bindingi202Divalent metal cation 2; catalyticUniRule annotation2 Publications1
Binding sitei220Substrate1 Publication1
Metal bindingi234Divalent metal cation 1UniRule annotation2 Publications1
Metal bindingi234Divalent metal cation 2; catalyticUniRule annotation2 Publications1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:RP824
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000002480 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489501 – 259Methionine aminopeptidaseAdd BLAST259

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272947.RP824.

Structurei

Secondary structure

1259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 28Combined sources21
Helixi29 – 31Combined sources3
Helixi38 – 51Combined sources14
Turni57 – 60Combined sources4
Helixi61 – 63Combined sources3
Beta strandi66 – 72Combined sources7
Beta strandi75 – 77Combined sources3
Beta strandi91 – 100Combined sources10
Beta strandi103 – 112Combined sources10
Helixi118 – 136Combined sources19
Helixi144 – 157Combined sources14
Beta strandi168 – 170Combined sources3
Beta strandi172 – 181Combined sources10
Beta strandi198 – 201Combined sources4
Beta strandi204 – 208Combined sources5
Beta strandi212 – 214Combined sources3
Turni216 – 218Combined sources3
Beta strandi222 – 224Combined sources3
Beta strandi230 – 232Combined sources3
Beta strandi234 – 239Combined sources6
Beta strandi241 – 247Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MR1X-ray2.00A/B/C/D3-249[»]
3MX6X-ray1.70A/B3-259[»]
ProteinModelPortaliQ9ZCD3.
SMRiQ9ZCD3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ZCD3.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA1. Bacteria.
COG0024. LUCA.
HOGENOMiHOG000030427.
KOiK01265.
OMAiCQLTARC.

Family and domain databases

CDDicd01086. MetAP1. 1 hit.
Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZCD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIKIHTEKD FIKMRAAGKL AAETLDFITD HVKPNVTTNS LNDLCHNFIT
60 70 80 90 100
SHNAIPAPLN YKGFPKSICT SINHVVCHGI PNDKPLKNGD IVNIDVTVIL
110 120 130 140 150
DGWYGDTSRM YYVGDVAIKP KRLIQVTYDA MMKGIEVVRP GAKLGDIGYA
160 170 180 190 200
IQSYAEKHNY SVVRDYTGHG IGRVFHDKPS ILNYGRNGTG LTLKEGMFFT
210 220 230 240 250
VEPMINAGNY DTILSKLDGW TVTTRDKSLS AQFEHTIGVT KDGFEIFTLS

PKKLDYPPY
Length:259
Mass (Da):28,861
Last modified:May 1, 1999 - v1
Checksum:i2CBFE88F0DD67B61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235273 Genomic DNA. Translation: CAA15249.1.
PIRiA71644.
RefSeqiNP_221173.1. NC_000963.1.
WP_004599671.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15249; CAA15249; CAA15249.
GeneIDi883940.
KEGGirpr:RP824.
PATRICi17902518. VBIRicPro72556_0860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235273 Genomic DNA. Translation: CAA15249.1.
PIRiA71644.
RefSeqiNP_221173.1. NC_000963.1.
WP_004599671.1. NC_000963.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MR1X-ray2.00A/B/C/D3-249[»]
3MX6X-ray1.70A/B3-259[»]
ProteinModelPortaliQ9ZCD3.
SMRiQ9ZCD3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP824.

Protein family/group databases

MEROPSiM24.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA15249; CAA15249; CAA15249.
GeneIDi883940.
KEGGirpr:RP824.
PATRICi17902518. VBIRicPro72556_0860.

Phylogenomic databases

eggNOGiENOG4105CA1. Bacteria.
COG0024. LUCA.
HOGENOMiHOG000030427.
KOiK01265.
OMAiCQLTARC.

Miscellaneous databases

EvolutionaryTraceiQ9ZCD3.

Family and domain databases

CDDicd01086. MetAP1. 1 hit.
Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAP1_RICPR
AccessioniPrimary (citable) accession number: Q9ZCD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.