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Q9ZCD3

- MAP1_RICPR

UniProt

Q9ZCD3 - MAP1_RICPR

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Protein

Methionine aminopeptidase

Gene

map

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781SubstrateUniRule annotation
Metal bindingi95 – 951Divalent metal cation 1
Metal bindingi106 – 1061Divalent metal cation 1
Metal bindingi106 – 1061Divalent metal cation 2; catalytic
Metal bindingi169 – 1691Divalent metal cation 2; catalytic; via tele nitrogen
Binding sitei176 – 1761Substrate
Metal bindingi202 – 2021Divalent metal cation 2; catalytic
Binding sitei220 – 2201Substrate
Metal bindingi234 – 2341Divalent metal cation 1
Metal bindingi234 – 2341Divalent metal cation 2; catalytic

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:RP824
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Methionine aminopeptidasePRO_0000148950Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272947.RP824.

Structurei

Secondary structure

1
259
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2821
Helixi29 – 313
Helixi38 – 5114
Turni57 – 604
Helixi61 – 633
Beta strandi66 – 727
Beta strandi75 – 773
Beta strandi91 – 10010
Beta strandi103 – 11210
Helixi118 – 13619
Helixi144 – 15714
Beta strandi168 – 1703
Beta strandi172 – 18110
Beta strandi198 – 2014
Beta strandi204 – 2085
Beta strandi212 – 2143
Turni216 – 2183
Beta strandi222 – 2243
Beta strandi230 – 2323
Beta strandi234 – 2396
Beta strandi241 – 2477

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MR1X-ray2.00A/B/C/D3-249[»]
3MX6X-ray1.70A/B3-259[»]
ProteinModelPortaliQ9ZCD3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ZCD3.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0024.
HOGENOMiHOG000030427.
KOiK01265.
OMAiMPGDRPL.
OrthoDBiEOG6MWNDS.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZCD3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTIKIHTEKD FIKMRAAGKL AAETLDFITD HVKPNVTTNS LNDLCHNFIT
60 70 80 90 100
SHNAIPAPLN YKGFPKSICT SINHVVCHGI PNDKPLKNGD IVNIDVTVIL
110 120 130 140 150
DGWYGDTSRM YYVGDVAIKP KRLIQVTYDA MMKGIEVVRP GAKLGDIGYA
160 170 180 190 200
IQSYAEKHNY SVVRDYTGHG IGRVFHDKPS ILNYGRNGTG LTLKEGMFFT
210 220 230 240 250
VEPMINAGNY DTILSKLDGW TVTTRDKSLS AQFEHTIGVT KDGFEIFTLS

PKKLDYPPY
Length:259
Mass (Da):28,861
Last modified:May 1, 1999 - v1
Checksum:i2CBFE88F0DD67B61
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ235273 Genomic DNA. Translation: CAA15249.1.
PIRiA71644.
RefSeqiNP_221173.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15249; CAA15249; CAA15249.
GeneIDi883940.
KEGGirpr:RP824.
PATRICi17902518. VBIRicPro72556_0860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ235273 Genomic DNA. Translation: CAA15249.1 .
PIRi A71644.
RefSeqi NP_221173.1. NC_000963.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MR1 X-ray 2.00 A/B/C/D 3-249 [» ]
3MX6 X-ray 1.70 A/B 3-259 [» ]
ProteinModelPortali Q9ZCD3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272947.RP824.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA15249 ; CAA15249 ; CAA15249 .
GeneIDi 883940.
KEGGi rpr:RP824.
PATRICi 17902518. VBIRicPro72556_0860.

Phylogenomic databases

eggNOGi COG0024.
HOGENOMi HOG000030427.
KOi K01265.
OMAi MPGDRPL.
OrthoDBi EOG6MWNDS.

Miscellaneous databases

EvolutionaryTracei Q9ZCD3.

Family and domain databases

Gene3Di 3.90.230.10. 1 hit.
HAMAPi MF_01974. MetAP_1.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 1 hit.
TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
PROSITEi PS00680. MAP_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.
  2. "Crystal structure of methionine aminopeptidase from Rickettsia prowazekii."
    Seattle structural genomics center for infectious disease (SSGCID)
    Edwards T.E., Abendroth J., Sankaran B.
    Submitted (APR-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 3-249 IN COMPLEX WITH MANGANESE IONS.
  3. "Crystal structure of methionine aminopeptidase from Rickettsia prowazekii bound to methionine."
    Seattle Structural Genomics Center for Infectious Disease (SSGCID)
    Edwards T.E., Abendroth J., Arakaki T., Sankaran B.
    Submitted (MAY-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 3-259 IN COMPLEX WITH MANGANESE IONS AND METHIONINE.

Entry informationi

Entry nameiMAP1_RICPR
AccessioniPrimary (citable) accession number: Q9ZCD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3