Reviewed, UniProtKB/Swiss-Prot Q9ZCB8 (HEM1_RICPR)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthase

Gene names

Name:	hemA
Ordered Locus Names:	RP841

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	5-aminolevulinate synthase		PRO_0000163831
Amino acid modifications
	Modified residue	244	1	N6-(pyridoxal phosphate)lysine Probable

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCB8-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 9763C4E1966A2822	FASTA	414	46,516
	10 20 30 40 50 60 MSYYDTIFNK HIDKIKSEGR YREFKSLKRQ ADNFPFAEYE DKQIVMWCIN DYLGMSKHVK 70 80 90 100 110 120 VMQASIDALL KYGVGSGGTR NIGGNNISIL ELEKELADLH SKETALVFTS GFVANDTTLA 130 140 150 160 170 180 SLAKIIPDIV FFSDELNHAS IIAGIKSSRA EKYVYRHLDV QHLEKLLQSV DINKPKIIVF 190 200 210 220 230 240 ESAYSMDGFF SPIKDIINLA KKYNALTFID EVHTVGLYGK QGGGISELLD CSNQIDIIQG 250 260 270 280 290 300 TLAKAYGTIG GYITSNYNLI DAIRLTAPGF IFTTSLPPVI STAATHSIRH LKESNEERIK 310 320 330 340 350 360 HQEVVTKLKN SFEHFNIPYL KNESHIIPII IGDPIKATKV SNMLLNEYGI YVQHINFPTV 370 380 390 400 410 PRGTERLRII PTPAHTDKMI NDLSTALVHI FDELDIELSS AKELNKEVRL HLIA

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235273 Genomic DNA. Translation: CAA15265.1.
PIR	A71646.
RefSeq	NP_221189.1.
3D structure databases
SMR	Q9ZCB8. Positions 4-391.
ModBase	Search...
Genome annotation databases
GeneID	883292.
KEGG	rpr:RP841.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649839.
OMA	HLKESNE.
Enzyme and pathway databases
BioCyc	RPRO272947:RP841-MONOMER.
BRENDA	2.3.1.37. 141977.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_RICPR

Accession

Primary (citable) accession number: Q9ZCB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)