Q9ZCB7 (TIG_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trigger factor
Short name=TF
EC=5.2.1.8

Alternative name(s):
PPIase

Gene names

Name:	tig
Ordered Locus Names:	RP842

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase By similarity. HAMAP-Rule MF_00303
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0). HAMAP-Rule MF_00303
Subcellular location	Cytoplasm. Note: About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm By similarity. HAMAP-Rule MF_00303
Domain	Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own By similarity. HAMAP-Rule MF_00303
Sequence similarities	Belongs to the FKBP-type PPIase family. Tig subfamily. Contains 1 PPIase FKBP-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm
Molecular function	Chaperone Isomerase Rotamase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from electronic annotation. Source: HAMAP protein peptidyl-prolyl isomerization Inferred from electronic annotation. Source: GOC protein transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Trigger factor HAMAP-Rule MF_00303		PRO_0000179417
Regions
	Domain	162 – 247	86	PPIase FKBP-type

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCB7 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 86676A8A1BA56A08	FASTA	445	51,135
	10 20 30 40 50 60 MGIIVLKNEG LNFHARISTP LSEIDDDIQK ELLDLTKKVK VAGFRAGKVP VSIVKKKYGT 70 80 90 100 110 120 SVRHDIIEKR INNLVNHIIK EYNLNIIGRP KIEELQNEPD KDLEFTVKIE LLPKITIPDL 130 140 150 160 170 180 KKISLDRPKL AVNSQDVEIQ LEKLAALTKC YTKESKTKIK DGDQVTIDAI GYIKDRAFDG 190 200 210 220 230 240 GKLNDFKVVI GSNTLIQGFE QQLIGSKTGN EVDVNVTFPE NYHDKNLSGK DAHFVVQIKA 250 260 270 280 290 300 VHTAEPTIID EEFAKKFQSN SLEELRTHFA KQIENESEEA INTIMKMNLF DKLEKLLDFD 310 320 330 340 350 360 VPESLLEQEK NILKSETDKN KHDGSLLNGK SSKEITEYYN KLALRRVRIG LLLAEYAKFK 370 380 390 400 410 420 NLQLEPDDFK KIIMQQARNF PGQENMIFDF YKNNPRAIEG LKGPALEDKT VQYIFNNEIQ 430 440 LKEKRYTKEE LEKYLETEEQ RISLI

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235273 Genomic DNA. Translation: CAA15266.1.
PIR	B71646.
RefSeq	NP_221190.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZCB7.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP842.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA15266; CAA15266; CAA15266.
GeneID	883899.
KEGG	rpr:RP842.
PATRIC	17902560. VBIRicPro72556_0880.
Phylogenomic databases
eggNOG	COG0544.
HOGENOM	HOG000218241.
KO	K03545.
OMA	YIKEKAF.
ProtClustDB	PRK01490.
Family and domain databases
Gene3D	1.10.3120.10. 1 hit. 3.30.70.1050. 1 hit.
HAMAP	MF_00303. Trigger_factor_Tig.
InterPro	IPR001179. PPIase_FKBP_dom. IPR005215. Trig_fac. IPR008880. Trigger_fac_C. IPR008881. Trigger_fac_ribosome-bd_bac. IPR027304. Trigger_fact/SurA_dom. [Graphical view]
Pfam	PF00254. FKBP_C. 1 hit. PF05698. Trigger_C. 1 hit. PF05697. Trigger_N. 1 hit. [Graphical view]
PIRSF	PIRSF003095. Trigger_factor. 1 hit.
SUPFAM	SSF109998. Trigger_fac_C_bac. 1 hit. SSF102735. Trigger_fac_ribosome-bd_bac. 1 hit.
TIGRFAMs	TIGR00115. tig. 1 hit.
PROSITE	PS50059. FKBP_PPIASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIG_RICPR

Accession

Primary (citable) accession number: Q9ZCB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program