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Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631S-adenosyl-L-methionineBy similarity
Binding sitei192 – 1921S-adenosyl-L-methionineBy similarity
Binding sitei207 – 2071S-adenosyl-L-methionineBy similarity
Binding sitei347 – 3471S-adenosyl-L-methionineBy similarity
Binding sitei372 – 3721S-adenosyl-L-methionineBy similarity
Binding sitei399 – 3991S-adenosyl-L-methionineBy similarity
Active sitei421 – 4211By similarity
Binding sitei421 – 4211S-adenosyl-L-methionineBy similarity
Binding sitei425 – 4251SubstrateBy similarity
Binding sitei457 – 4571SubstrateBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. protein methyltransferase activity Source: InterPro
  3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
M.RprHemKP
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RP847
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518Bifunctional methyltransferasePRO_0000157174Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272947.RP847.

Structurei

3D structure databases

ProteinModelPortaliQ9ZCB3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 302302RF MTaseAdd
BLAST
Regioni1 – 300300HemKAdd
BLAST
Regioni140 – 1445S-adenosyl-L-methionine bindingBy similarity
Regioni207 – 2104Substrate bindingBy similarity
Regioni301 – 518218tRNA (guanine-N(7)-)-methyltransferaseAdd
BLAST
Regioni305 – 518214tRNA MTaseAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000010896.
KOiK02493.
OMAiQQNLLDN.
OrthoDBiEOG68Q0SZ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZCB3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQYSIKQILS NANDKLNKIG INLPGLEARI LLQHVTNKPI EHLLIKLNEQ
60 70 80 90 100
LSEAEIEAFE KLLERRLAHE PIAYIIGVKE FYSREFIVNK HVLIPRIDTE
110 120 130 140 150
VLVDVVIGLV VSRNNLHMFS KLKSLDSVLT TQSYNILELG TGSGCIAISL
160 170 180 190 200
LCELPNTNII ATDISVDAIK VAKSNSIKYN VTDRIQIIHS NWFEKLDKQK
210 220 230 240 250
FDFIVSNPPY ISHTEKLKMA IETINYEPSI ALFAEEDGLE AYSIIAKNAK
260 270 280 290 300
QFLKPNGKII LEIGFSQAAK VSKIFLNYGY NIDYIYRDLQ SHNRVIEISP
310 320 330 340 350
INLNRSYARR IGKSLSKMQQ KLLDNELPKY LFSKEKFKSE KRKVFLEIGF
360 370 380 390 400
GMGEHLINQA KINPDTLFIG VEVYLNGVAN VLKHSAQHNI TNFLLFPNNL
410 420 430 440 450
DLILNDLPNN SLDGIYILFP DPWIKNKKKK KRIFNKERLK ILQNKLKNNG
460 470 480 490 500
NLVFASDIEN YFYEAMALIR QNGNFEIIHN DDYLQPHDNY IITKYHQKAI
510
NANRTAKFMI LQHALTDH
Length:518
Mass (Da):59,564
Last modified:May 1, 1999 - v1
Checksum:iC66BB8DB273704A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235273 Genomic DNA. Translation: CAA15271.1.
PIRiG71646.
RefSeqiNP_221195.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15271; CAA15271; CAA15271.
GeneIDi883464.
KEGGirpr:RP847.
PATRICi17902570. VBIRicPro72556_0885.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235273 Genomic DNA. Translation: CAA15271.1.
PIRiG71646.
RefSeqiNP_221195.1. NC_000963.1.

3D structure databases

ProteinModelPortaliQ9ZCB3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA15271; CAA15271; CAA15271.
GeneIDi883464.
KEGGirpr:RP847.
PATRICi17902570. VBIRicPro72556_0885.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000010896.
KOiK02493.
OMAiQQNLLDN.
OrthoDBiEOG68Q0SZ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.

Entry informationi

Entry nameiRFTRM_RICPR
AccessioniPrimary (citable) accession number: Q9ZCB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: January 7, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.