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Q9ZCB3

- RFTRM_RICPR

UniProt

Q9ZCB3 - RFTRM_RICPR

Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei163 – 1631S-adenosyl-L-methionineBy similarity
    Binding sitei192 – 1921S-adenosyl-L-methionineBy similarity
    Binding sitei207 – 2071S-adenosyl-L-methionineBy similarity
    Binding sitei347 – 3471S-adenosyl-L-methionineBy similarity
    Binding sitei372 – 3721S-adenosyl-L-methionineBy similarity
    Binding sitei399 – 3991S-adenosyl-L-methionineBy similarity
    Active sitei421 – 4211By similarity
    Binding sitei421 – 4211S-adenosyl-L-methionineBy similarity
    Binding sitei425 – 4251SubstrateBy similarity
    Binding sitei457 – 4571SubstrateBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. protein methyltransferase activity Source: InterPro
    3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional methyltransferase
    Including the following 2 domains:
    Release factor glutamine methyltransferase (EC:2.1.1.297)
    Short name:
    RF MTase
    Alternative name(s):
    M.RprHemKP
    N5-glutamine methyltransferase PrmC
    Protein-(glutamine-N5) MTase PrmC
    Protein-glutamine N-methyltransferase PrmC
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:prmC/trmB
    Synonyms:hemK
    Ordered Locus Names:RP847
    OrganismiRickettsia prowazekii (strain Madrid E)
    Taxonomic identifieri272947 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000002480: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 518518Bifunctional methyltransferasePRO_0000157174Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272947.RP847.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZCB3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 302302RF MTaseAdd
    BLAST
    Regioni1 – 300300HemKAdd
    BLAST
    Regioni140 – 1445S-adenosyl-L-methionine bindingBy similarity
    Regioni207 – 2104Substrate bindingBy similarity
    Regioni301 – 518218tRNA (guanine-N(7)-)-methyltransferaseAdd
    BLAST
    Regioni305 – 518214tRNA MTaseAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
    In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000010896.
    KOiK02493.
    OMAiQQNLLDN.
    OrthoDBiEOG68Q0SZ.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZCB3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQYSIKQILS NANDKLNKIG INLPGLEARI LLQHVTNKPI EHLLIKLNEQ    50
    LSEAEIEAFE KLLERRLAHE PIAYIIGVKE FYSREFIVNK HVLIPRIDTE 100
    VLVDVVIGLV VSRNNLHMFS KLKSLDSVLT TQSYNILELG TGSGCIAISL 150
    LCELPNTNII ATDISVDAIK VAKSNSIKYN VTDRIQIIHS NWFEKLDKQK 200
    FDFIVSNPPY ISHTEKLKMA IETINYEPSI ALFAEEDGLE AYSIIAKNAK 250
    QFLKPNGKII LEIGFSQAAK VSKIFLNYGY NIDYIYRDLQ SHNRVIEISP 300
    INLNRSYARR IGKSLSKMQQ KLLDNELPKY LFSKEKFKSE KRKVFLEIGF 350
    GMGEHLINQA KINPDTLFIG VEVYLNGVAN VLKHSAQHNI TNFLLFPNNL 400
    DLILNDLPNN SLDGIYILFP DPWIKNKKKK KRIFNKERLK ILQNKLKNNG 450
    NLVFASDIEN YFYEAMALIR QNGNFEIIHN DDYLQPHDNY IITKYHQKAI 500
    NANRTAKFMI LQHALTDH 518
    Length:518
    Mass (Da):59,564
    Last modified:May 1, 1999 - v1
    Checksum:iC66BB8DB273704A1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ235273 Genomic DNA. Translation: CAA15271.1.
    PIRiG71646.
    RefSeqiNP_221195.1. NC_000963.1.

    Genome annotation databases

    EnsemblBacteriaiCAA15271; CAA15271; CAA15271.
    GeneIDi883464.
    KEGGirpr:RP847.
    PATRICi17902570. VBIRicPro72556_0885.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ235273 Genomic DNA. Translation: CAA15271.1 .
    PIRi G71646.
    RefSeqi NP_221195.1. NC_000963.1.

    3D structure databases

    ProteinModelPortali Q9ZCB3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272947.RP847.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA15271 ; CAA15271 ; CAA15271 .
    GeneIDi 883464.
    KEGGi rpr:RP847.
    PATRICi 17902570. VBIRicPro72556_0885.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000010896.
    KOi K02493.
    OMAi QQNLLDN.
    OrthoDBi EOG68Q0SZ.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Madrid E.

    Entry informationi

    Entry nameiRFTRM_RICPR
    AccessioniPrimary (citable) accession number: Q9ZCB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Rickettsia prowazekii
      Rickettsia prowazekii (strain Madrid E): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3