Q9ZCB1 (SYGB_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	RP849

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	665 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence CAA15273.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 665	665	Glycine--tRNA ligase beta subunit HAMAP-Rule MF_00255		PRO_0000072924

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCB1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: A193D931CE7463F5	FASTA	665	76,216
	10 20 30 40 50 60 MVSELLLELF SEEIPAFMQK NAEEGYLKIF TKIFEENEIF AKVQVFAGPR RITLHATHVP 70 80 90 100 110 120 KVILPKEEEI KGPSIEAPEI AINGFCKAHN VSKLDLSTKL RNNKLYYFFV KKTKERETKE 130 140 150 160 170 180 ILPKIIIDAI NKYSWTKSMF WGCYKIKWIR PLRNILCIFD GEILPLRFGH LSTNNITYGH 190 200 210 220 230 240 RLTNNKKLEI IDFENYRNKL LENNVILERL KREEIIKVGL LELANAQNLN IKQDVCLIEE 250 260 270 280 290 300 VTGLSEFPIV LLGKIPQKFL ELPEEVIVSV MRTHQKYFCL FDKNGSFAPY FLFVINGRFV 310 320 330 340 350 360 NTELIIQGNE KVLSARLADA LYFYKNDIAK TLESRLDKLK SVIFHTKLGS LKEKVDRITD 370 380 390 400 410 420 ICRYIAPDNI DLIMAAKLCK SDLVSDMVGE FPDLQGIMGY YYAKHEELNE EVAKAIRDHY 430 440 450 460 470 480 KPQGLNDNVP SGNATLLALA DKLDSLVGLI IVGETPNGSG DPYALRRQAL GIIRIIIENK 490 500 510 520 530 540 LEINFINLIS FSVSLYKVSS NSHLDSVISF FEERAKFYFK NDYDITLINA VLDLNLVDTK 550 560 570 580 590 600 FKLDTLKEFL VQDVGKQLLN AYKRVSNIMG NQKITGLVDA SLFSTQYEKE LFEVIQKISQ 610 620 630 640 650 660 QIIVIIANKD YQKALNLLSS LLKPITSFFD NVLVNDSDPK IAQNRLSLLQ NTCEVFDKVA KFCRL

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235273 Genomic DNA. Translation: CAA15273.1. Different initiation.
PIR	A71647.
RefSeq	NP_221197.2. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZCB1.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP849.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA15273; CAA15273; CAA15273.
GeneID	883343.
KEGG	rpr:RP849.
PATRIC	17902574. VBIRicPro72556_0887.
Phylogenomic databases
eggNOG	COG0751.
HOGENOM	HOG000264304.
KO	K01879.
ProtClustDB	PRK01233.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta.
InterPro	IPR008909. DALR_anticod-bd. IPR015944. Gly-tRNA-synth_bsu. IPR006194. Gly-tRNA-synth_heterodimer. [Graphical view]
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00211. glyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_RICPR

Accession

Primary (citable) accession number: Q9ZCB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program