Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9ZCA3 (TRUA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase A

EC=5.4.99.12
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene names
Name:truA
Ordered Locus Names:RP857
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171

Catalytic activity

tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00171

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Sequence caution

The sequence CAA15281.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245tRNA pseudouridine synthase A HAMAP-Rule MF_00171
PRO_0000057441

Sites

Active site521Nucleophile By similarity
Binding site1111Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZCA3 [UniParc].

Last modified October 19, 2002. Version 2.
Checksum: 9062DA2C8539BF46

FASTA24528,170
        10         20         30         40         50         60 
MYRYKITIEY LGTHFAGWQR QAGVLSVQQI LEEAIYKFSS EQVTLFGSGR TDAGVHAIGQ 

        70         80         90        100        110        120 
VAHFDLSKYL EPYKIIKAIN YFVRPYDVGV WNCELVSNNF HARFSAISRH YIYRIINRTY 

       130        140        150        160        170        180 
PSVIDFNRAW WISSPLDILA MQKAAAYLLG KHDFTSFRSS SCQSKSPIKT LTEINIIKEY 

       190        200        210        220        230        240 
EEIKLYISAP SFLHYMVRNI VGSLVLVGKN IWQAEQIKNV LDARDRKIAG PTAPAFGLYF 


IKAEY 

« Hide

References

[1]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ235273 Genomic DNA. Translation: CAA15281.1. Different initiation.
PIRA71648.
RefSeqNP_221205.1. NC_000963.1.

3D structure databases

ProteinModelPortalQ9ZCA3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272947.RP857.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA15281; CAA15281; CAA15281.
GeneID883320.
KEGGrpr:RP857.
PATRIC17902592. VBIRicPro72556_0896.

Phylogenomic databases

eggNOGCOG0101.
HOGENOMHOG000248672.
KOK06173.
OrthoDBEOG6Z9B4R.
ProtClustDBPRK00021.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPMF_00171. TruA.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUA_RICPR
AccessionPrimary (citable) accession number: Q9ZCA3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 19, 2002
Last modified: February 19, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names