Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Oxygen-dependent coproporphyrinogen-III oxidase

Gene

hemF

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.UniRule annotation

Catalytic activityi

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Oxygen-dependent coproporphyrinogen-III oxidase (hemF)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102SubstrateUniRule annotation1
Metal bindingi106Divalent metal cationUniRule annotation1
Active sitei116Proton donorUniRule annotation1
Metal bindingi116Divalent metal cationUniRule annotation1
Metal bindingi149Divalent metal cationUniRule annotation1
Metal bindingi179Divalent metal cationUniRule annotation1
Sitei179Important for dimerizationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandMetal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00322.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)
Short name:
CPOUniRule annotation
Short name:
Coprogen oxidaseUniRule annotation
Short name:
CoproporphyrinogenaseUniRule annotation
Gene namesi
Name:hemFUniRule annotation
Ordered Locus Names:RP882
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000002480 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001099161 – 279Oxygen-dependent coproporphyrinogen-III oxidaseAdd BLAST279

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi272947.RP882.

Structurei

3D structure databases

ProteinModelPortaliQ9ZC86.
SMRiQ9ZC86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 120Substrate bindingUniRule annotation3
Regioni244 – 279Important for dimerizationUniRule annotationAdd BLAST36

Sequence similaritiesi

Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DBS. Bacteria.
COG0408. LUCA.
HOGENOMiHOG000262768.
KOiK00228.
OMAiMDLTPYY.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas. 1 hit.
InterProiView protein in InterPro
IPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiView protein in Pfam
PF01218. Coprogen_oxidas. 1 hit.
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiView protein in PROSITE
PS01021. COPROGEN_OXIDASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZC86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTENKEITS NWFTNLRDLL CKEFEKIEEK YAQIKGLKPA KFVRTSWKRN
60 70 80 90 100
GGGCGIMSLM KGEVFEKVGV NISTVFGEFS QEFRSEILGA ELDGKFFATG
110 120 130 140 150
ISVVAHLKSP LIPAMHFNTR YIETSKNWFG GGGDLTPFYP EENETAKFHT
160 170 180 190 200
AFKEACDKYD SSYYPKFKKQ CDEYFYLRHR KEPRGVGGIF YDYLNSGNFE
210 220 230 240 250
QDFAFTKDIG KALLSVYPEI VRSKLFLPWT AEQKEYQLIR RGRYVEFNLL
260 270
YDRGTKFGLM TDGNVEAILM SLPPVVKFN
Length:279
Mass (Da):32,197
Last modified:May 1, 1999 - v1
Checksum:i334D2FFDA55220AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235273 Genomic DNA. Translation: CAA15304.1.
PIRiH71650.
RefSeqiNP_221228.1. NC_000963.1.
WP_004596721.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15304; CAA15304; CAA15304.
GeneIDi883468.
KEGGirpr:RP882.
PATRICifig|272947.5.peg.922.

Similar proteinsi

Entry informationi

Entry nameiHEM6_RICPR
AccessioniPrimary (citable) accession number: Q9ZC86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 1999
Last modified: September 27, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families