Reviewed, UniProtKB/Swiss-Prot Q9ZBU1 (XYOA_STRCO)

Names and origin

Protein names

Recommended name:
    Probable xylitol oxidase
    EC=1.1.3.41

Gene names

Name:	xyoA
Ordered Locus Names:	SCO6147
ORF Names:	SC1A9.11c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of xylitol to xylose, also acts on D-sorbitol By similarity.
Catalytic activity	Xylitol + O(2) = xylose + H(2)O(2).
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the oxygen-dependent FAD-linked oxidoreductase family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	xylitol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 418

418

Probable xylitol oxidase

PRO_0000128174

Regions

Domain

13 – 179

167

FAD-binding PCMH-type

Amino acid modifications

Modified residue

46

1

Tele-8alpha-FAD histidine By similarity

Secondary structure

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418

Helix Strand Turn

Details...

Beta strand

9 – 12

4

Helix

26 – 35

10

Beta strand

37 – 41

5

Beta strand

59 – 61

3

Beta strand

70 – 73

4

Turn

74 – 77

4

Beta strand

78 – 82

5

Helix

87 – 96

10

Beta strand

106 – 108

3

Helix

112 – 117

6

Helix

130 – 133

4

Beta strand

134 – 140

7

Beta strand

146 – 150

5

Helix

156 – 158

3

Turn

163 – 166

4

Beta strand

168 – 175

8

Beta strand

181 – 190

10

Helix

197 – 204

8

Beta strand

206 – 214

9

Beta strand

216 – 229

14

Beta strand

240 – 242

3

Helix

255 – 257

3

Helix

268 – 270

3

Beta strand

272 – 275

4

Beta strand

287 – 294

8

Helix

295 – 297

3

Helix

298 – 307

10

Helix

309 – 312

4

Helix

313 – 315

3

Beta strand

316 – 324

9

Beta strand

339 – 346

8

Helix

350 – 364

15

Helix

365 – 367

3

Helix

381 – 385

5

Helix

391 – 401

11

Helix

410 – 416

7

Sequences

	Sequence		Length	Mass (Da)
	Q9ZBU1-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2A80DF7735A87DCD	FASTA	418	44,347
	10 20 30 40 50 60 MSDITVTNWA GNITYTAKEL LRPHSLDALR ALVADSARVR VLGSGHSFNE IAEPGDGGVL 70 80 90 100 110 120 LSLAGLPSVV DVDTAARTVR VGGGVRYAEL ARVVHARGLA LPNMASLPHI SVAGSVATGT 130 140 150 160 170 180 HGSGVGNGSL ASVVREVELV TADGSTVVIA RGDERFGGAV TSLGALGVVT SLTLDLEPAY 190 200 210 220 230 240 EMEQHVFTEL PLAGLDPATF ETVMAAAYSV SLFTDWRAPG FRQVWLKRRT DRPLDGFPYA 250 260 270 280 290 300 APAAEKMHPV PGMPAVNCTE QFGVPGPWHE RLPHFRAEFT PSSGAELQSE YLMPREHALA 310 320 330 340 350 360 ALHAMDAIRE TLAPVLQTCE IRTVAADAQW LSPAYGRDTV AAHFTWVEDT AAVLPVVRRL 370 380 390 400 410 EEALVPFAAR PHWGKVFTVP AGELRALYPR LADFGALAGA LDPAGKFTNA FVRGVLAG

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AL939126 Genomic DNA. Translation: CAA22381.1.

PIR

T34660.

RefSeq

NP_630252.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VFR	X-ray	1.10	A	1-418	[»]
2VFS	X-ray	1.60	A	1-418	[»]
2VFT	X-ray	1.60	A	1-418	[»]
2VFU	X-ray	1.90	A	1-418	[»]
2VFV	X-ray	1.72	A	1-418	[»]

ModBase

Search...

Genome annotation databases

GeneID

1101588.

GenomeReviews

Gene locus SCO6147 in contig AL645882_GR.

KEGG

sco:SCO6147.

NMPDR

fig|100226.1.peg.6092.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q9ZBU1.

Enzyme and pathway databases

BioCyc

SCOE100226:SCO6147-MON.

Family and domain databases

InterPro

IPR016168. FAD-linked_Oxase_FAD-bd_sub2.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
[Graphical view]

Gene3D

G3DSA:3.30.465.20. FAD-linked_oxidase_FAD-bd_sub2. 1 hit.

Pfam

PF01565. FAD_binding_4. 1 hit.
[Graphical view]

PROSITE

PS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. False negative.
[Graphical view]

BLOCKS

Search...

ProtoNet

Search...

Entry information

Entry name

XYOA_STRCO

Accession

Primary (citable) accession number: Q9ZBU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	May 1, 1999
Last modified:	November 4, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)