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Protein

Ribonuclease 3

Gene

rnc

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. May modulate key aspects of gene expression as its absence has extensive effects on the abundance of about 200 different transcripts. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.3 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70MagnesiumBy similarity1
Active sitei74Sequence analysis1
Metal bindingi143MagnesiumBy similarity1
Active sitei146By similarity1
Metal bindingi146MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding, rRNA-binding
Biological processmRNA processing, rRNA processing, tRNA processing
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.26.3. 5998.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Antibiotic biosynthesis protein B
Short name:
AbsB
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Synonyms:absB
Ordered Locus Names:SCO5572
ORF Names:SC7A1.16
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

Q9ZBQ7:

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Not essential. Deficient in endogenous antibiotic synthesis, able to form a sporulating aerial mycelium. Accumulates a 30S rRNA precursor transcript. Leads to increased expression of pnp due to increased transcript levels.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi172L → P in strain C120; loss of endogenous antibiotic synthesis, decrease in transcript abundance of genes involved in secondary metabolism. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001804401 – 276Ribonuclease 3Add BLAST276

Proteomic databases

PRIDEiQ9ZBQ7.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi100226.SCO5572.

Structurei

3D structure databases

SMRiQ9ZBQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 157RNase IIIAdd BLAST127
Domaini184 – 252DRBMAdd BLAST69

Sequence similaritiesi

Belongs to the ribonuclease III family.Curated

Phylogenomic databases

eggNOGiENOG4108ZBM. Bacteria.
COG0571. LUCA.
HOGENOMiHOG000246808.
InParanoidiQ9ZBQ7.
KOiK03685.
OrthoDBiPOG091H00R1.

Family and domain databases

CDDicd00593. RIBOc. 1 hit.
Gene3Di1.10.1520.10. 1 hit.
HAMAPiMF_00104. RNase_III. 1 hit.
InterProiView protein in InterPro
IPR014720. dsRBD_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
IPR036389. RNase_III_sf.
PfamiView protein in Pfam
PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
SMARTiView protein in SMART
SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiView protein in PROSITE
PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZBQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGTVSVPKK AEDAKADPPA KKKADTQASS HTLLEGRLGY QLESALLVRA
60 70 80 90 100
LTHRSYAYEN GGLPTNERLE FLGDSVLGLV VTDTLYRTHP DLPEGQLAKL
110 120 130 140 150
RAAVVNSRAL AEVGRGLELG SFIRLGRGEE GTGGRDKASI LADTLEAVIG
160 170 180 190 200
AVYLDQGLDA ASELVHRLFD PLIEKSSNLG AGLDWKTSLQ ELTATEGLGV
210 220 230 240 250
PEYLVTETGP DHEKTFTAAA RVGGVSYGTG TGRSKKEAEQ QAAESAWRSI
260 270
RAAADERAKA TADAVDADPD EASASA
Length:276
Mass (Da):29,141
Last modified:April 18, 2012 - v2
Checksum:iD0C69BEABF62313C
GO

Sequence cautioni

Q9ZBQ7: The sequence CAA22415 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939124 Genomic DNA. Translation: CAA22415.1. Different initiation.
PIRiT35656.
RefSeqiNP_629707.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA22415; CAA22415; CAA22415.
GeneIDi1101013.
KEGGisco:SCO5572.
PATRICifig|100226.15.peg.5661.

Similar proteinsi

Entry informationi

Entry nameiRNC_STRCO
AccessioniPrimary (citable) accession number: Q9ZBQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 18, 2012
Last modified: October 25, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families