Ribonuclease 3 - Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

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Q9ZBQ7 (RNC_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Antibiotic biosynthesis protein B
Short name=AbsB
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Synonyms:	absB
Ordered Locus Names:	SCO5572
ORF Names:	SC7A1.16

Organism

Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]

Taxonomic identifier

100226 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces ›

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. May modulate key aspects of gene expression as its absence has extensive effects on the abundance of about 200 different transcripts. Ref.1 Ref.3 Ref.4
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg²⁺ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Disruption phenotype	Not essential. Deficient in endogenous antibiotic synthesis, able to form a sporulating aerial mycelium. Accumulates a 30S rRNA precursor transcript. Leads to increased expression of pnp due to increased transcript levels. Ref.1 Ref.4
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.
Sequence caution	The sequence CAA22415.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 276

276

Ribonuclease 3 HAMAP-Rule MF_00104

PRO_0000180440

Regions

Domain

31 – 157

127

RNase III

Domain

184 – 252

DRBM

Sites

Active site

Potential

Active site

146

By similarity

Metal binding

Magnesium By similarity

Metal binding

143

Magnesium By similarity

Metal binding

146

Magnesium By similarity

Experimental info

Mutagenesis

172

L → P in strain C120; loss of endogenous antibiotic synthesis, decrease in transcript abundance of genes involved in secondary metabolism. Ref.1 Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ZBQ7 [UniParc].

Last modified April 18, 2012. Version 2.
Checksum: D0C69BEABF62313C
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FASTA

276

29,141

        10         20         30         40         50         60 
MRGTVSVPKK AEDAKADPPA KKKADTQASS HTLLEGRLGY QLESALLVRA LTHRSYAYEN 

        70         80         90        100        110        120 
GGLPTNERLE FLGDSVLGLV VTDTLYRTHP DLPEGQLAKL RAAVVNSRAL AEVGRGLELG 

       130        140        150        160        170        180 
SFIRLGRGEE GTGGRDKASI LADTLEAVIG AVYLDQGLDA ASELVHRLFD PLIEKSSNLG 

       190        200        210        220        230        240 
AGLDWKTSLQ ELTATEGLGV PEYLVTETGP DHEKTFTAAA RVGGVSYGTG TGRSKKEAEQ 

       250        260        270 
QAAESAWRSI RAAADERAKA TADAVDADPD EASASA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Streptomyces coelicolor antibiotic regulatory gene, absB, encodes an RNase III homolog." Price B., Adamidis T., Kong R., Champness W. J. Bacteriol. 181:6142-6151(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PROCESSING OF RRNA, DISRUPTION PHENOTYPE, MUTAGENESIS OF LEU-172. Strain: ATCC BAA-471 / A3(2) / M145.
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. Hopwood D.A. Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[3]	"Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor." Huang J., Shi J., Molle V., Sohlberg B., Weaver D., Bibb M.J., Karoonuthaisiri N., Lih C.J., Kao C.M., Buttner M.J., Cohen S.N. Mol. Microbiol. 58:1276-1287(2005) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN GLOBAL GENE REGULATION, MUTAGENESIS OF LEU-172. Strain: ATCC BAA-471 / A3(2) / M145.
[4]	"RNase III-dependent expression of the rpsO-pnp operon of Streptomyces coelicolor." Gatewood M.L., Bralley P., Jones G.H. J. Bacteriol. 193:4371-4379(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS AN ENDORIBONUCLEASE, FUNCTION IN PROCESSING OF MRNA, DISRUPTION PHENOTYPE. Strain: ATCC BAA-471 / A3(2) / M145.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939124 Genomic DNA. Translation: CAA22415.1. Different initiation.
PIR	T35656.
RefSeq	NP_629707.1. NC_003888.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	100226.SCO5572.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA22415; CAA22415; CAA22415.
GeneID	1101013.
KEGG	sco:SCO5572.
PATRIC	23741020. VBIStrCoe124346_5661.
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246808.
KO	K03685.
ProtClustDB	PRK00102.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_STRCO

Accession

Primary (citable) accession number: Q9ZBQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 18, 2012
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents