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Q9ZBQ7

- RNC_STRCO

UniProt

Q9ZBQ7 - RNC_STRCO

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Protein

Ribonuclease 3

Gene

rnc

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. May modulate key aspects of gene expression as its absence has extensive effects on the abundance of about 200 different transcripts. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.3 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701MagnesiumBy similarity
Active sitei74 – 741Sequence Analysis
Metal bindingi143 – 1431MagnesiumBy similarity
Active sitei146 – 1461By similarity
Metal bindingi146 – 1461MagnesiumBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Antibiotic biosynthesis protein B
Short name:
AbsB
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Synonyms:absB
Ordered Locus Names:SCO5572
ORF Names:SC7A1.16
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Not essential. Deficient in endogenous antibiotic synthesis, able to form a sporulating aerial mycelium. Accumulates a 30S rRNA precursor transcript. Leads to increased expression of pnp due to increased transcript levels.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi172 – 1721L → P in strain C120; loss of endogenous antibiotic synthesis, decrease in transcript abundance of genes involved in secondary metabolism. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Ribonuclease 3PRO_0000180440Add
BLAST

Proteomic databases

PRIDEiQ9ZBQ7.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi100226.SCO5572.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 157127RNase IIIAdd
BLAST
Domaini184 – 25269DRBMAdd
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.Curated

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246808.
InParanoidiQ9ZBQ7.
KOiK03685.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZBQ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRGTVSVPKK AEDAKADPPA KKKADTQASS HTLLEGRLGY QLESALLVRA
60 70 80 90 100
LTHRSYAYEN GGLPTNERLE FLGDSVLGLV VTDTLYRTHP DLPEGQLAKL
110 120 130 140 150
RAAVVNSRAL AEVGRGLELG SFIRLGRGEE GTGGRDKASI LADTLEAVIG
160 170 180 190 200
AVYLDQGLDA ASELVHRLFD PLIEKSSNLG AGLDWKTSLQ ELTATEGLGV
210 220 230 240 250
PEYLVTETGP DHEKTFTAAA RVGGVSYGTG TGRSKKEAEQ QAAESAWRSI
260 270
RAAADERAKA TADAVDADPD EASASA
Length:276
Mass (Da):29,141
Last modified:April 18, 2012 - v2
Checksum:iD0C69BEABF62313C
GO

Sequence cautioni

The sequence CAA22415.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939124 Genomic DNA. Translation: CAA22415.1. Different initiation.
PIRiT35656.
RefSeqiNP_629707.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA22415; CAA22415; CAA22415.
GeneIDi1101013.
KEGGisco:SCO5572.
PATRICi23741020. VBIStrCoe124346_5661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939124 Genomic DNA. Translation: CAA22415.1 . Different initiation.
PIRi T35656.
RefSeqi NP_629707.1. NC_003888.3.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO5572.

Proteomic databases

PRIDEi Q9ZBQ7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA22415 ; CAA22415 ; CAA22415 .
GeneIDi 1101013.
KEGGi sco:SCO5572.
PATRICi 23741020. VBIStrCoe124346_5661.

Phylogenomic databases

eggNOGi COG0571.
HOGENOMi HOG000246808.
InParanoidi Q9ZBQ7.
KOi K03685.
OrthoDBi EOG6T1WVS.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Streptomyces coelicolor antibiotic regulatory gene, absB, encodes an RNase III homolog."
    Price B., Adamidis T., Kong R., Champness W.
    J. Bacteriol. 181:6142-6151(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PROCESSING OF RRNA, DISRUPTION PHENOTYPE, MUTAGENESIS OF LEU-172.
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor."
    Huang J., Shi J., Molle V., Sohlberg B., Weaver D., Bibb M.J., Karoonuthaisiri N., Lih C.J., Kao C.M., Buttner M.J., Cohen S.N.
    Mol. Microbiol. 58:1276-1287(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN GLOBAL GENE REGULATION, MUTAGENESIS OF LEU-172.
    Strain: ATCC BAA-471 / A3(2) / M145.
  4. "RNase III-dependent expression of the rpsO-pnp operon of Streptomyces coelicolor."
    Gatewood M.L., Bralley P., Jones G.H.
    J. Bacteriol. 193:4371-4379(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN ENDORIBONUCLEASE, FUNCTION IN PROCESSING OF MRNA, DISRUPTION PHENOTYPE.
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiRNC_STRCO
AccessioniPrimary (citable) accession number: Q9ZBQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 18, 2012
Last modified: November 26, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3