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Q9ZBQ7

- RNC_STRCO

UniProt

Q9ZBQ7 - RNC_STRCO

Protein

Ribonuclease 3

Gene

rnc

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (18 Apr 2012)
      Previous versions | rss
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    Functioni

    Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. May modulate key aspects of gene expression as its absence has extensive effects on the abundance of about 200 different transcripts. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.3 Publications

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.

    Cofactori

    Mg2+.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701MagnesiumBy similarity
    Active sitei74 – 741Sequence Analysis
    Metal bindingi143 – 1431MagnesiumBy similarity
    Active sitei146 – 1461By similarity
    Metal bindingi146 – 1461MagnesiumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonuclease III activity Source: UniProtKB-HAMAP
    3. rRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-HAMAP
    2. rRNA catabolic process Source: InterPro
    3. rRNA processing Source: UniProtKB-HAMAP
    4. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    mRNA processing, rRNA processing, tRNA processing

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding, rRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 3 (EC:3.1.26.3)
    Alternative name(s):
    Antibiotic biosynthesis protein B
    Short name:
    AbsB
    Ribonuclease III
    Short name:
    RNase III
    Gene namesi
    Name:rnc
    Synonyms:absB
    Ordered Locus Names:SCO5572
    ORF Names:SC7A1.16
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Not essential. Deficient in endogenous antibiotic synthesis, able to form a sporulating aerial mycelium. Accumulates a 30S rRNA precursor transcript. Leads to increased expression of pnp due to increased transcript levels.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi172 – 1721L → P in strain C120; loss of endogenous antibiotic synthesis, decrease in transcript abundance of genes involved in secondary metabolism. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 276276Ribonuclease 3PRO_0000180440Add
    BLAST

    Proteomic databases

    PRIDEiQ9ZBQ7.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi100226.SCO5572.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 157127RNase IIIAdd
    BLAST
    Domaini184 – 25269DRBMAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RNase III domain.Curated

    Phylogenomic databases

    eggNOGiCOG0571.
    HOGENOMiHOG000246808.
    KOiK03685.
    OrthoDBiEOG6T1WVS.

    Family and domain databases

    Gene3Di1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPiMF_00104. RNase_III.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PANTHERiPTHR11207. PTHR11207. 1 hit.
    PfamiPF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69065. SSF69065. 1 hit.
    TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZBQ7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRGTVSVPKK AEDAKADPPA KKKADTQASS HTLLEGRLGY QLESALLVRA    50
    LTHRSYAYEN GGLPTNERLE FLGDSVLGLV VTDTLYRTHP DLPEGQLAKL 100
    RAAVVNSRAL AEVGRGLELG SFIRLGRGEE GTGGRDKASI LADTLEAVIG 150
    AVYLDQGLDA ASELVHRLFD PLIEKSSNLG AGLDWKTSLQ ELTATEGLGV 200
    PEYLVTETGP DHEKTFTAAA RVGGVSYGTG TGRSKKEAEQ QAAESAWRSI 250
    RAAADERAKA TADAVDADPD EASASA 276
    Length:276
    Mass (Da):29,141
    Last modified:April 18, 2012 - v2
    Checksum:iD0C69BEABF62313C
    GO

    Sequence cautioni

    The sequence CAA22415.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939124 Genomic DNA. Translation: CAA22415.1. Different initiation.
    PIRiT35656.
    RefSeqiNP_629707.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAA22415; CAA22415; CAA22415.
    GeneIDi1101013.
    KEGGisco:SCO5572.
    PATRICi23741020. VBIStrCoe124346_5661.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939124 Genomic DNA. Translation: CAA22415.1 . Different initiation.
    PIRi T35656.
    RefSeqi NP_629707.1. NC_003888.3.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO5572.

    Proteomic databases

    PRIDEi Q9ZBQ7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA22415 ; CAA22415 ; CAA22415 .
    GeneIDi 1101013.
    KEGGi sco:SCO5572.
    PATRICi 23741020. VBIStrCoe124346_5661.

    Phylogenomic databases

    eggNOGi COG0571.
    HOGENOMi HOG000246808.
    KOi K03685.
    OrthoDBi EOG6T1WVS.

    Family and domain databases

    Gene3Di 1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPi MF_00104. RNase_III.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    PANTHERi PTHR11207. PTHR11207. 1 hit.
    Pfami PF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69065. SSF69065. 1 hit.
    TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Streptomyces coelicolor antibiotic regulatory gene, absB, encodes an RNase III homolog."
      Price B., Adamidis T., Kong R., Champness W.
      J. Bacteriol. 181:6142-6151(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PROCESSING OF RRNA, DISRUPTION PHENOTYPE, MUTAGENESIS OF LEU-172.
      Strain: ATCC BAA-471 / A3(2) / M145.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    3. "Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor."
      Huang J., Shi J., Molle V., Sohlberg B., Weaver D., Bibb M.J., Karoonuthaisiri N., Lih C.J., Kao C.M., Buttner M.J., Cohen S.N.
      Mol. Microbiol. 58:1276-1287(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN GLOBAL GENE REGULATION, MUTAGENESIS OF LEU-172.
      Strain: ATCC BAA-471 / A3(2) / M145.
    4. "RNase III-dependent expression of the rpsO-pnp operon of Streptomyces coelicolor."
      Gatewood M.L., Bralley P., Jones G.H.
      J. Bacteriol. 193:4371-4379(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN ENDORIBONUCLEASE, FUNCTION IN PROCESSING OF MRNA, DISRUPTION PHENOTYPE.
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiRNC_STRCO
    AccessioniPrimary (citable) accession number: Q9ZBQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: April 18, 2012
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3