Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9ZAE1

- PNP_THETH

UniProt

Q9ZAE1 - PNP_THETH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Thermus thermophilus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi498 – 4981MagnesiumUniRule annotation
Metal bindingi504 – 5041MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
OrganismiThermus thermophilus
Taxonomic identifieri274 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 713713Polyribonucleotide nucleotidyltransferasePRO_0000329915Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9ZAE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini565 – 63167KHUniRule annotationAdd
BLAST
Domaini633 – 70169S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZAE1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGTPNVPQA HRYELTLAGR PLVLETGKYA KQASGSVLVR YADTVVLATA
60 70 80 90 100
QASETPVEAD FLPLTVEFEE RHYAVGKIPG SFMRREGRPG EKAILSARMT
110 120 130 140 150
DRPIRPLFPK GFRHEVQIIV TVLSADQKNP PDILGPIAAS AALMLSDIPW
160 170 180 190 200
EGPIAAVRVG LIGGSFVLNP TLQELEESQL DLVVAGSKEA ILMVEAEAGE
210 220 230 240 250
VDEETLVQAL EFAHKEMQPI LELQEAMARE LAKPKMAWTP PESLPEEEKE
260 270 280 290 300
ALYRLALERG LSQVLQTASK GERSRALAEF AERLIAEALP KGEDGTPDEG
310 320 330 340 350
KKPLYESAFD EVVRRELRRL VLEEGKRADG RGPKDLRPIW IEVDVLPRAH
360 370 380 390 400
GSAVFTRGET QVLGTVTLGT GRDEQILDDL GIDETEKFLV HYNFPPFSTG
410 420 430 440 450
EVRRLRGVSR REVGHGNLAK RALKAVMPKE EDFPYTIRVV GDVLESNGSS
460 470 480 490 500
SMATVCAGCL ALMDAGVPIR APVAGVAMGL VWEENRAVIL TDILGLEDAL
510 520 530 540 550
GDMDFKVAGT RKGVTALQMD NKVGGLPREV LKEALLQARE ARLKILDLME
560 570 580 590 600
TVLPAPRPEL KPFAPRILSL KVPVEKIGLV IGPGGKNVRA LEELGVEVDI
610 620 630 640 650
EEDGTVRIYS SDLNAALEAK KRIEDLTREA KVGEIYEGTV TRITPFGAFI
660 670 680 690 700
SLFPGTEGLL HISQIAPGRV ERVEDHLKVG DVIKVKVHRI DERGKIDLIR
710
PELEGKIPPR RRK
Length:713
Mass (Da):78,193
Last modified:May 1, 1999 - v1
Checksum:i2979859D9AC5EA82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z84207 Genomic DNA. Translation: CAB06341.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z84207 Genomic DNA. Translation: CAB06341.1 .

3D structure databases

ProteinModelPortali Q9ZAE1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Polynucleotide phosphorylase from Thermus thermophilus: cloning, sequencing and expression of the gene and biochemical properties of the enzyme."
    Serganov A.A., Garber M.B., Portier C.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: VK1.

Entry informationi

Entry nameiPNP_THETH
AccessioniPrimary (citable) accession number: Q9ZAE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3