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Protein

ATP-dependent Clp protease proteolytic subunit

Gene

clpP

Organism
Lactococcus lactis subsp. cremoris (strain MG1363)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.UniRule annotation

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991NucleophileUniRule annotation
Active sitei124 – 1241UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • misfolded or incompletely synthesized protein catabolic process Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-669-MONOMER.
BRENDAi3.4.21.92. 2903.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunitUniRule annotation (EC:3.4.21.92UniRule annotation)
Alternative name(s):
Endopeptidase ClpUniRule annotation
Gene namesi
Name:clpPUniRule annotation
Ordered Locus Names:llmg_0638
OrganismiLactococcus lactis subsp. cremoris (strain MG1363)
Taxonomic identifieri416870 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000000364 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199ATP-dependent Clp protease proteolytic subunitPRO_0000179574Add
BLAST

Expressioni

Inductioni

By heat shock.

Interactioni

Subunit structurei

Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.UniRule annotation

Protein-protein interaction databases

STRINGi416870.llmg_0638.

Structurei

3D structure databases

ProteinModelPortaliQ9ZAB0.
SMRiQ9ZAB0. Positions 4-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCQ. Bacteria.
COG0740. LUCA.
HOGENOMiHOG000285833.
KOiK01358.
OMAiARMNELM.
OrthoDBiEOG6Z3KQ0.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZAB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYLVPTVIE QSSRGERAYD IYSRLLKDRI IMLTGPVEDG MANSIIAQLL
60 70 80 90 100
FLDAQDNTKD IYLYVNTPGG SVSAGLAIVD TMNFIKSDVQ TIVMGMAASM
110 120 130 140 150
GTIIASSGTK GKRFMLPNAE YLIHQPMGGA GQGTQQTDMA IVAEQLLKTR
160 170 180 190
KRLEQILADN SNRSLEQIHK DAERDHWMDA KETLEYGFID EIMENNSLK
Length:199
Mass (Da):22,052
Last modified:March 1, 2001 - v2
Checksum:iF2CC13835891CCFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF028804 Genomic DNA. Translation: AAD01866.2.
AM406671 Genomic DNA. Translation: CAL97240.1.
RefSeqiWP_011834647.1. NC_009004.1.

Genome annotation databases

EnsemblBacteriaiCAL97240; CAL97240; llmg_0638.
KEGGillm:llmg_0638.
PATRICi22282376. VBILacLac4574_0653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF028804 Genomic DNA. Translation: AAD01866.2.
AM406671 Genomic DNA. Translation: CAL97240.1.
RefSeqiWP_011834647.1. NC_009004.1.

3D structure databases

ProteinModelPortaliQ9ZAB0.
SMRiQ9ZAB0. Positions 4-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416870.llmg_0638.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL97240; CAL97240; llmg_0638.
KEGGillm:llmg_0638.
PATRICi22282376. VBILacLac4574_0653.

Phylogenomic databases

eggNOGiENOG4105CCQ. Bacteria.
COG0740. LUCA.
HOGENOMiHOG000285833.
KOiK01358.
OMAiARMNELM.
OrthoDBiEOG6Z3KQ0.

Enzyme and pathway databases

BioCyciLLAC416870:GCDT-669-MONOMER.
BRENDAi3.4.21.92. 2903.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ClpP participates in the degradation of misfolded protein in Lactococcus lactis."
    Frees D., Ingmer H.
    Mol. Microbiol. 31:79-87(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363."
    Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.
    J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MG1363.

Entry informationi

Entry nameiCLPP_LACLM
AccessioniPrimary (citable) accession number: Q9ZAB0
Secondary accession number(s): A2RIZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: March 16, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.