Q9Z9X0 (GATB_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	BH0667

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00121
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP MF_00121		PRO_0000148760

Sequences

	Sequence		Length	Mass (Da)
	Q9Z9X0 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: C212B522413C579B	FASTA	476	53,415
	10 20 30 40 50 60 MNFETVIGLE VHVELKTESK IFSASPNHFG AEPNANTSVI DLGYPGVLPV LNKAAVEFAM 70 80 90 100 110 120 KAAMALNCEV ATDTKFDRKN YFYPDNPKAY QISQFDKPIG ENGWIEIEVD GTKKKIGITR 130 140 150 160 170 180 LHLEEDAGKL THSGNGYSLV DFNRQGTPLI EIVSEPDIRT PQEAYAYLEK LKSIIQYTGV 190 200 210 220 230 240 SDCKMEEGSL RCDANISLRP VGQEEFGTKT ELKNLNSFNF VRKGLEYEEK RQAQVLLSGG 250 260 270 280 290 300 EILQETRRYD EAANKTVLMR VKEGSDDYRY FPEPDLVALH IDDEWKARIR SEIPELPDAR 310 320 330 340 350 360 KKRYVEELGL PAYDAMVLTL TKEMSDFFEE TIAKGADPKL ASNWLMGEVS GYLNAEQKEL 370 380 390 400 410 420 DEVALTPDGL AKMIQLIEKG TISSKIAKKV FKDLIEKGGD PEEIVKAKGL VQISDEGELR 430 440 450 460 470 KYVVEVLDNN QQSIDDFKNG KDRAIGFLVG QIMKATKGKA NPPMVNKLLL EEINKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of three lambda clones from the genome of alkaliphilic Bacillus sp. strain C-125." Takami H., Nakasone K., Ogasawara N., Hirama C., Nakamura Y., Masui N., Fuji F., Takaki Y., Inoue A., Horikoshi K. Extremophiles 3:29-34(1999) [PubMed: 10086842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
[2]	"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011836 Genomic DNA. Translation: BAA75312.1. BA000004 Genomic DNA. Translation: BAB04386.1.
PIR	T44293.
RefSeq	NP_241533.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9Z9X0.
SMR	Q9Z9X0. Positions 2-412.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000052034; EBBACP00000050673; EBBACG00000052025.
GeneID	893061.
GenomeReviews	Gene locus BH0667 in contig BA000004_GR.
KEGG	bha:BH0667.
NMPDR	fig|272558.1.peg.667.
PATRIC	18938322. VBIBacHal18977_0699.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002603.
HOGENOM	HBG395951.
OMA	KNYFYAD.
PhylomeDB	Q9Z9X0.
ProtClustDB	PRK05477.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0667-MONOMER.
Family and domain databases
HAMAP	MF_00121. GatB. [Tree]
InterPro	IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR004413. Gln-tRNA_amidoTrfase_bsu. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
KO	K02434.
PANTHER	PTHR11659. GatB. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. GatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_BACHD

Accession

Primary (citable) accession number: Q9Z9X0
Secondary accession number(s): Q9JPV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program