Q9Z9W9 (GATA_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	BH0666

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00120
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP MF_00120		PRO_0000105136
Sites
	Active site	79	1	Charge relay system By similarity
	Active site	154	1	Charge relay system By similarity
	Active site	178	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z9W9 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 914C7A27D0FB9167	FASTA	485	52,888
	10 20 30 40 50 60 MSLFDLKLKD VHTKLHEKEI SVSDLVDEAY KRIEQVDGQV EAFLALNEEK ARAYAKELDA 70 80 90 100 110 120 ALDRSEARGL LFGIPIGVKD NIVTKNLRTT CSSRILGNFD PIYDATVVHK LREAQAVTIG 130 140 150 160 170 180 KLNMDEFAMG SSTENSAFQK TKNPWNLEYV PGGSSGGSAA AVAAGEVPFT LGSDTGGSIR 190 200 210 220 230 240 QPAAYCGVVG LKPTYGRVSR YGLVAFASSL DQIGPITRNV EDNAYLLQAI SGHDPMDSTS 250 260 270 280 290 300 ANLDVPDYLS ALTGDIKGLK IAVPKEYLGE GVKEEVKQSV LDALKVLEGL GATWEEVSLP 310 320 330 340 350 360 HSKYALATYY LLASSEASAN LARFDGVRYG FRSDNADNLL DMYKQTRAEG FGDEVKRRIM 370 380 390 400 410 420 LGTFALSSGY YDAYYKKAQQ VRTLIKQDFE KVFEQYDVII GPTTPTPAFK IGEKTDDPLT 430 440 450 460 470 480 MYANDILTIP VNLAGVPAIS VPCGFDNGLP LGLQIIGKHF DEGSVYRVAH AFEQATDYHT KRPTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of three lambda clones from the genome of alkaliphilic Bacillus sp. strain C-125." Takami H., Nakasone K., Ogasawara N., Hirama C., Nakamura Y., Masui N., Fuji F., Takaki Y., Inoue A., Horikoshi K. Extremophiles 3:29-34(1999) [PubMed: 10086842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
[2]	"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011836 Genomic DNA. Translation: BAA75313.1. BA000004 Genomic DNA. Translation: BAB04385.1.
PIR	T44294.
RefSeq	NP_241532.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9Z9W9.
SMR	Q9Z9W9. Positions 1-479.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000053212; EBBACP00000051851; EBBACG00000053203.
GeneID	893055.
GenomeReviews	Gene locus BH0666 in contig BA000004_GR.
KEGG	bha:BH0666.
NMPDR	fig|272558.1.peg.666.
PATRIC	18938320. VBIBacHal18977_0698.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002886.
HOGENOM	HBG481888.
OMA	TESSCYG.
PhylomeDB	Q9Z9W9.
ProtClustDB	PRK00012.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0666-MONOMER.
Family and domain databases
HAMAP	MF_00120. GatA. [Tree]
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
Gene3D	G3DSA:3.90.1300.10. Amidase_sig_enz. 1 hit.
KO	K02433.
PANTHER	PTHR11895. Amidase. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. GatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_BACHD

Accession

Primary (citable) accession number: Q9Z9W9
Secondary accession number(s): Q9JPV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program