Reviewed, UniProtKB/Swiss-Prot Q9Z9U1 (DHSO_BACHD)

Names and origin

Protein names

Recommended name:
    Sorbitol dehydrogenase
    EC=1.1.1.14
Alternative name(s):
    L-iditol 2-dehydrogenase
    Glucitol dehydrogenase

Gene names

Name:	gutB
Ordered Locus Names:	BH0189

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces glucitol to fructose By similarity.
Catalytic activity	L-iditol + NAD(+) = L-sorbose + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-iditol 2-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Sorbitol dehydrogenase		PRO_0000160824
Sites
	Metal binding	39	1	Zinc 1; catalytic By similarity
	Metal binding	60	1	Zinc 1; catalytic By similarity
	Metal binding	91	1	Zinc 2 By similarity
	Metal binding	94	1	Zinc 2 By similarity
	Metal binding	97	1	Zinc 2 By similarity
	Metal binding	105	1	Zinc 2 By similarity
	Metal binding	146	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z9U1-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 20961F7C34676DFA	FASTA	343	36,889
	10 20 30 40 50 60 MKALVKTQHG TGHFAVQEKP EPTPGKHQVK IKVKYTGVCG SDIHTYEGHY PVAAPVTLGH 70 80 90 100 110 120 EFSGEIVELG EGVTGFNVGD RVTSETTYSI CGKCSYCTSG DYNLCSHRKG LGNQQDGSFA 130 140 150 160 170 180 KYVIARQESL HHLPAGVDDR SAAMTEPLAC THHAIAKTSI NKGDLVVVTG PGPIGLLAAQ 190 200 210 220 230 240 VAKSHGGTVI ITGLSNDQVR LKKAKEVGID YAIDTQEVDI KELVSELTDG YGADVVLECS 250 260 270 280 290 300 GAVPAAKQGI DLLRKKGQYA QVGLFAQPEI QFNFEKIIQK EISVVGSRSQ KPADWEPALS 310 320 330 340 LLNEKKVNAK TLVTHEYTIS EWDKAYHAIK SGEAIKVLLT PID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of three lambda clones from the genome of alkaliphilic Bacillus sp. strain C-125." Takami H., Nakasone K., Ogasawara N., Hirama C., Nakamura Y., Masui N., Fuji F., Takaki Y., Inoue A., Horikoshi K. Extremophiles 3:29-34(1999) [PubMed: 10086842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.
[2]	"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB011837 Genomic DNA. Translation: BAA75341.1. BA000004 Genomic DNA. Translation: BAB03908.1.
PIR	E83673.
RefSeq	NP_241055.1.
3D structure databases
HSSP	HSSP built from PDB template 1JVB based on UniProtKB P39462.
ModBase	Search...
Genome annotation databases
GeneID	891766.
GenomeReviews	Gene locus BH0189 in contig BA000004_GR.
KEGG	bha:BH0189.
NMPDR	fig|272558.1.peg.189.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Z9U1.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0189-MON.
Family and domain databases
InterPro	IPR013154. AlcDHase_GroES-like. IPR002085. AlcDHase_SF_Zn. IPR013149. AlcDHase_Zn-bd. IPR002328. AlcDHase_Zn_CS. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProDom	PD040557. GroES_related. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

DHSO_BACHD

Accession

Primary (citable) accession number: Q9Z9U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 1, 1999
Last modified:	November 4, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)