Q9Z9M2 (RPOB_BACHD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	BH0126

Organism

Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) [Complete proteome] [HAMAP]

Taxonomic identifier

272558 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	1180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1180	1180	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047859

Sequences

	Sequence		Length	Mass (Da)
	Q9Z9M2 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 00E1517AE4D07970	FASTA	1,180	132,638
	10 20 30 40 50 60 MTGQLIQYGR HRQRRSYARI NEVLELPNLI EIQTASYQWF LDEGLREMFQ DISPIQDFTG 70 80 90 100 110 120 NLVLEFIDYS LGEPKYPVDE SKERDVTYAA PLRVKVRLIN KETGEVKEQE VFMGDFPLMT 130 140 150 160 170 180 DTGTFIINGA ERVIVSQLVR SPSVYYSEKI DKNGKKGYTA TVIPNRGAWL ELETDAKDIV 190 200 210 220 230 240 YVRIDRTRKI PVTVLLRALG FGSDQEIIDL LGEDEYLRNT LEKDNTDSAE KALLEIYERL 250 260 270 280 290 300 RPGEPPTVEN AKSLLDSRFF DPKRYDLANV GRYKVNKKLH IKNRLFNQRL AETLIDPETG 310 320 330 340 350 360 EVIAEEGSII DRRTLDRILP YLENNVGFRT VRMSGGVVEE DEVRLQSVKI YAPDDQDGEH 370 380 390 400 410 420 VIRVIGNGLV EKEIKHITPA DIIASINYFF NLLHSVGGTD DIDHLGNRRL RSVGELLQNQ 430 440 450 460 470 480 FRIGLSRMER VVRERMSIQD PNSITPQALI NIRPVIASIK EFFGSSQLSQ FMDQTNPLAE 490 500 510 520 530 540 LTHKRRLSAL GPGGLTRERA GFEVRDVHYS HYGRMCPIET PEGPNIGLIN SLSTYAKVNE 550 560 570 580 590 600 FGFMETPYRR VDPETGKVTA QIDYLTADEE DNYVVAQANM KLAEDGSFID ENIIARFRGE 610 620 630 640 650 660 NIVVSRDRVD YMDVSPKQVV SAATSCIPFL ENDDSNRALM GANMQRQAVP LLVPEAPIVG 670 680 690 700 710 720 TGMEHVSAKD SGAAIVSKHR GIVERVTAKE IWVRRLEEVD GKEIKGDLDK YRLQKFIRSN 730 740 750 760 770 780 QGTSYNQRPI VKEGDVVEKR EILADGPSME KGEMALGRNV LVGFMTWEGY NYEDAIILSE 790 800 810 820 830 840 RLVKDDVYTS IHIEEYESEA RDTKLGPEEI TRDIPNVGED ALRNLDERGI IRVGAEVKDG 850 860 870 880 890 900 DILVGKVTPK GVTELTAEER LLHAIFGEKA REVRDTSLRA PHGGDGIVLD VKIFNREDGD 910 920 930 940 950 960 ELPPGVNQLV RVYIVQKRKI HEGDKMAGRH GNKGVISRIL PEEDMPYLPD GTPIDIMLNP 970 980 990 1000 1010 1020 LGVPSRMNIG QVLELHLGMA ARRLGLHVAS PVFDGASEED VWATLEEAGM ARDGKTILYD 1030 1040 1050 1060 1070 1080 GRTGEPFDNR VSVGIMYMIK LAHMVDDKLH ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME 1090 1100 1110 1120 1130 1140 VWALEAYGAA YTLQEILTVK SDDVVGRVKT YEAIVKGENV PEPGVPESFK VLIKELQSLG 1150 1160 1170 1180 MDVKMLSSTE EEIEMKELDD EDEQASDKLN LNIDSTESNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 32-kb region including the major ribosomal protein gene clusters from alkaliphilic Bacillus sp. strain C-125." Takami H., Takaki Y., Nakasone K., Hirama C., Inoue A., Horikoshi K. Biosci. Biotechnol. Biochem. 63:452-455(1999) [PubMed: 10192928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
[2]	"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017508 Genomic DNA. Translation: BAA75263.1. BA000004 Genomic DNA. Translation: BAB03845.1.
PIR	T44375.
RefSeq	NP_240992.1. NC_002570.2.
3D structure databases
ProteinModelPortal	Q9Z9M2.
ModBase	Search...
Proteomic databases
PRIDE	Q9Z9M2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000051801; EBBACP00000050440; EBBACG00000051792.
GeneID	892766.
GenomeReviews	Gene locus BH0126 in contig BA000004_GR.
KEGG	bha:BH0126.
NMPDR	fig|272558.1.peg.126.
PATRIC	18937124. VBIBacHal18977_0134.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001755.
HOGENOM	HBG285547.
OMA	VDARDTK.
PhylomeDB	Q9Z9M2.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0126-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 3 hits. G3DSA:2.40.50.150. Ribosomal_L2. 1 hit.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_BACHD

Accession

Primary (citable) accession number: Q9Z9M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program