Reviewed, UniProtKB/Swiss-Prot Q9Z9J4 (AMPM_BACHD)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	BH0156

Organism

Bacillus halodurans [Complete proteome] [HAMAP]

Taxonomic identifier

86665 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Methionine aminopeptidase		PRO_0000148925
Sites
	Metal binding	94	1	Cobalt 1 By similarity
	Metal binding	105	1	Cobalt 1 By similarity
	Metal binding	105	1	Cobalt 2 By similarity
	Metal binding	168	1	Cobalt 2 By similarity
	Metal binding	201	1	Cobalt 2 By similarity
	Metal binding	232	1	Cobalt 1 By similarity
	Metal binding	232	1	Cobalt 2 By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z9J4-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: C2295A5147447327	FASTA	248	27,155
	10 20 30 40 50 60 MIICKTEREL EIMREAGRIV ALTHQEIKLH IQPGITTKKL DEIAETFIRS HGATPSFKGY 70 80 90 100 110 120 NGFTGSICAS VNEELVHGIP GNRVLKEGDI ISVDIGAKYN GYHGDSAWTY AVGNISDEDQ 130 140 150 160 170 180 DLLDVTETSL YKGLEQAKAG ARLSDISHAI QSYAEPRGYA IVREYVGHGV GQNLHEDPQI 190 200 210 220 230 240 PHYGPPGKGP RLKPGMVLAI EPMVNAGSRY VRTLSDNWTV VTVDGKKCAH FEHTIAITET GYEILTKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 32-kb region including the major ribosomal protein gene clusters from alkaliphilic Bacillus sp. strain C-125." Takami H., Takaki Y., Nakasone K., Hirama C., Inoue A., Horikoshi K. Biosci. Biotechnol. Biochem. 63:452-455(1999) [PubMed: 10192928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.
[2]	"Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis." Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K. Nucleic Acids Res. 28:4317-4331(2000) [PubMed: 11058132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-125 / C-125 / DSM 18197 / FERM 7344 / JCM 9153.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB017508 Genomic DNA. Translation: BAA75293.1. BA000004 Genomic DNA. Translation: BAB03875.1.
PIR	T44405.
RefSeq	NP_241022.1.
3D structure databases
HSSP	HSSP built from PDB template 1O0X based on UniProtKB Q9X1I7.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Genome annotation databases
GeneID	893603.
GenomeReviews	Gene locus BH0156 in contig BA000004_GR.
KEGG	bha:BH0156.
NMPDR	fig|272558.1.peg.156.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Z9J4.
OMA	TATGPRI.
Enzyme and pathway databases
BioCyc	BHAL272558:BH0156-MON.
BRENDA	3.4.11.18. 191865.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_BACHD

Accession

Primary (citable) accession number: Q9Z9J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)