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Protein

Methionine aminopeptidase

Gene

map

Organism
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Metal bindingi94Divalent metal cation 1UniRule annotation1
Metal bindingi105Divalent metal cation 1UniRule annotation1
Metal bindingi105Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi168Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei175SubstrateUniRule annotation1
Metal bindingi201Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi232Divalent metal cation 1UniRule annotation1
Metal bindingi232Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:BH0156
OrganismiBacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Taxonomic identifieri272558 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489251 – 248Methionine aminopeptidaseAdd BLAST248

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272558.BH0156

Structurei

3D structure databases

ProteinModelPortaliQ9Z9J4
SMRiQ9Z9J4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA1 Bacteria
COG0024 LUCA
HOGENOMiHOG000030426
KOiK01265
OMAiSYFHGPP
OrthoDBiPOG091H01DX

Family and domain databases

CDDicd01086 MetAP1, 1 hit
HAMAPiMF_01974 MetAP_1, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002467 Pept_M24A_MAP1
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00500 met_pdase_I, 1 hit
PROSITEiView protein in PROSITE
PS00680 MAP_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z9J4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIICKTEREL EIMREAGRIV ALTHQEIKLH IQPGITTKKL DEIAETFIRS
60 70 80 90 100
HGATPSFKGY NGFTGSICAS VNEELVHGIP GNRVLKEGDI ISVDIGAKYN
110 120 130 140 150
GYHGDSAWTY AVGNISDEDQ DLLDVTETSL YKGLEQAKAG ARLSDISHAI
160 170 180 190 200
QSYAEPRGYA IVREYVGHGV GQNLHEDPQI PHYGPPGKGP RLKPGMVLAI
210 220 230 240
EPMVNAGSRY VRTLSDNWTV VTVDGKKCAH FEHTIAITET GYEILTKA
Length:248
Mass (Da):27,155
Last modified:May 1, 1999 - v1
Checksum:iC2295A5147447327
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017508 Genomic DNA Translation: BAA75293.1
BA000004 Genomic DNA Translation: BAB03875.1
PIRiT44405
RefSeqiWP_010896339.1, NC_002570.2

Genome annotation databases

EnsemblBacteriaiBAB03875; BAB03875; BAB03875
KEGGibha:BH0156

Similar proteinsi

Entry informationi

Entry nameiMAP1_BACHD
AccessioniPrimary (citable) accession number: Q9Z9J4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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