Q9Z9F4 (LON_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease
EC=3.4.21.53

Alternative name(s):
ATP-dependent protease La

Gene names

Name:	lon
Ordered Locus Names:	CPn_0027, CP_0749, CpB0031

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	819 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	By heat shock By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 819	819	Lon protease		PRO_0000076131
Regions
	Domain	42 – 238	197	Lon
	Nucleotide binding	393 – 400	8	ATP By similarity
Sites
	Active site	723	1	By similarity
	Active site	766	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z9F4 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 788BCB2A0D294E6A	FASTA	819	92,276
	10 20 30 40 50 60 MDSTTNSDSP ILDPNPEDVE KLLDESEEES EDQSTERLLP SELFILPLNK RPFFPGMAAP 70 80 90 100 110 120 ILIESGPYYE VLKVLAKSSQ KYIGLVLTKK ENADILKVSF NQLHKTGVAA RILRIMPIEG 130 140 150 160 170 180 GSAQVLLSIE ERIRIIEPIK DKYLKARVSY HADNKELTEE LKAYSISIVS VIKDLLKLNP 190 200 210 220 230 240 LFKEELQIFL GHSDFTEPGK LADFSVALTT ATREELQEVL ETTNMHDRID KALILLKKEL 250 260 270 280 290 300 DLSRLQSSIN QKIEATITKS QKEFFLKEQL KTIKKELGLE KEDRAIDIEK FSERLRKRHV 310 320 330 340 350 360 PDYAMEVIQD EIEKLQTLET SSAEYTVCRN YLDWLTIIPW GIQSKEYHDL KKAEIVLNKD 370 380 390 400 410 420 HYGLDEIKQR ILELISVGKL SKGLKGSIIC LVGPPGVGKT SIGRSIAKVL HRKFFRFSVG 430 440 450 460 470 480 GMRDEAEIKG HRRTYIGAMP GKMVQALKQS QAMNPVIMID EVDKIGASYH GDPASALLEV 490 500 510 520 530 540 LDPEQNKDFL DHYLDVRVDL SNVLFILTAN VLDTIPDPLL DRMEILRLSG YILEEKLQIA 550 560 570 580 590 600 KKYLVPKARK EIGLTASEVN FQPEALKYMI NNYAREAGVR TLNGNIKKVL RKVALKIVQN 610 620 630 640 650 660 QEKPKSKKIT FKISSKNLQT YLGKPIFSSD RFYESTPVGV ATGLAWTSLG GATLYIESVQ 670 680 690 700 710 720 VSSLKTDMHL TGQAGEVMKE SSQIAWTYLH SALHRYAPGY TFFPKSQVHI HIPEGATPKD 730 740 750 760 770 780 GPSAGITMVT SLLSLLLETP VVNNLGMTGE ITLTGRVLGV GGIREKLIAA RRSRLNILIF 790 800 810 PEDNRRDYEE LPAYLKTGLK IHFVSHYDDV LKVAFPKLK

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18180.1. AE002161 Genomic DNA. Translation: AAF38554.1. BA000008 Genomic DNA. Translation: BAA98239.1. AE009440 Genomic DNA. Translation: AAP97964.1.
PIR	B72128. E86494.
RefSeq	NP_224235.1. NC_000922.1. NP_300088.1. NC_002491.1. NP_445291.1. NC_002179.2. NP_876307.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z9F4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1466714. 894928. 918819. 963156.
GenomeReviews	Gene locus CPn_0027 in contig AE001363_GR. Gene locus CP_0749 in contig AE002161_GR. Gene locus CpB0031 in contig AE009440_GR. Gene locus lon in contig BA000008_GR.
KEGG	cpa:CP0749. cpn:CPn0027. cpt:CpB0031.
TIGR	CP_0749.
Phylogenomic databases
HOGENOM	HBG566281.
OMA	NIKNGIN.
ProtClustDB	CLSK2459232.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0749-MONOMER. CPNE115713:CPN0027-MONOMER. CPNE138677:CPJ0027-MONOMER. CPNE182082:CPB0031-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
KO	K01338.
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. Lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_CHLPN

Accession

Primary (citable) accession number: Q9Z9F4
Secondary accession number(s): Q9JQ69

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program