Q9Z9A0 (RPOB_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	CPn_0081, CP_0694, CpB0081

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	1252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.
Sequence caution	The sequence AAF38502.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1252	1252	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047880
Experimental info
	Sequence conflict	246	1	S → C in BAA98291. Ref.3
	Sequence conflict	259	1	T → I in BAA98291. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9Z9A0 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 83939598C1F1823A	FASTA	1,252	140,110
	10 20 30 40 50 60 MLKCPERVSV KKKEDIPDLP NLIEIQIKSY KQFLQIGKLA EERENIGLEE VFREIFPIKS 70 80 90 100 110 120 YNEATVLEYL SYNLGVPKYS PEECIRRGIT YSVTLKVRFR LTDETGIKEE EVYMGTIPLM 130 140 150 160 170 180 TDKGTFIING AERVVVSQVH RSPGINFEQE KHSKGNILFS FRIIPYRGSW LEAIFDINDL 190 200 210 220 230 240 IYIHIDRKKR RRKILAITFI RALGYSSDAD IIEEFFTIGE SSLRSEKDFA LLVGRILADN 250 260 270 280 290 300 IIDEASSLVY GKAGEKLSTA MLKRMLDAGI ASVKIAVDAD ENHPIIKMLA KDPTDSYEAA 310 320 330 340 350 360 LKDFYRRLRP GEPATLANAR STIMRLFFDP KRYNLGRVGR YKLNRKLGFS IDDEALSQVT 370 380 390 400 410 420 LRKEDVIGAL KYLIRLKMGD EKACVDDIDH LANRRVRSVG ELIQNQCRSG LARMEKIVRE 430 440 450 460 470 480 RMNLFDFSSD TLTPGKVVSA KGLASVLKDF FGRSQLSQFM DQTNPVAELT HKRRLSALGP 490 500 510 520 530 540 GGLNRERAGF EVRDVHASHY GRICPIETPE GPNIGLITSL SSFAKINEFG FIETPYRIVR 550 560 570 580 590 600 DGIVTDEIEY MTADVEEECV IAQASASLDE YNMFTEPVCW VRYAGEAFEA DTSTVTHMDV 610 620 630 640 650 660 SPKQLVSIVT GLIPFLEHDD ANRALMGSNM QRQAVPLLKT EAPVVGTGLE CRAAKDSGAI 670 680 690 700 710 720 VVAEEDGVVD FVDGYKVVVA AKHNPTIKRT YHLKKFLRSN SGTCINQQPL CAVGDVITKG 730 740 750 760 770 780 DVIADGPATD RGELALGKNV LVAFMPWYGY NFEDAIIISE KLIREDAYTS IYIEEFELTA 790 800 810 820 830 840 RDTKLGKEEI TRDIPNVSDE VLANLGEDGI IRIGAEVKPG DILVGKITPK SETELAPEER 850 860 870 880 890 900 LLRAIFGEKA ADVKDASLTV PPGTEGVVMD VKVFSRKDRL SKSDDELVEE AVHLKDLQKG 910 920 930 940 950 960 YKNQVATLKT EYREKLGALL LNEKAPAAII HRRTAEIVVH EGLLFDQETI ERIEQEDLVD 970 980 990 1000 1010 1020 LLMPNCEMYE VLKGLLSDYE TALQRLEINY KTEVEHIREG DADLDHGVIR QVKVYVASKR 1030 1040 1050 1060 1070 1080 KLQVGDKMAG RHGNKGVVSK IVPEADMPYL SNGETVQMIL NPLGVPSRMN LGQVLETHLG 1090 1100 1110 1120 1130 1140 YAAKTAGIYV KTPVFEGFPE QRIWDMMIEQ GLPEDGKSFL YDGKTGERFD NKVVIGYIYM 1150 1160 1170 1180 1190 1200 LKLSHLIADK IHARSIGPYS LVTQQPLGGK AQMGGQRFGE MEVWALEAYG VAHMLQEILT 1210 1220 1230 1240 1250 VKSDDVSGRT RIYESIVKGE NLLRSGTPES FNVLIKEMQG LGLDVRPMVV DA

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18234.1. AE002161 Genomic DNA. Translation: AAF38502.1. Different initiation. BA000008 Genomic DNA. Translation: BAA98291.1. AE009440 Genomic DNA. Translation: AAP98014.1.
PIR	A86501. D72122. F81548.
RefSeq	NP_224289.1. NC_000922.1. NP_300140.1. NC_002491.1. NP_445236.1. NC_002179.2. NP_876357.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z9A0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1466764. 894794. 918873. 963393.
GenomeReviews	Gene locus CPn_0081 in contig AE001363_GR. Gene locus CP_0694 in contig AE002161_GR. Gene locus CpB0081 in contig AE009440_GR. Gene locus rpoB in contig BA000008_GR.
KEGG	cpa:CP0694. cpn:CPn0081. cpt:CpB0081.
TIGR	CP_0694.
Phylogenomic databases
HOGENOM	HBG285547.
OMA	IGRNKTY.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0694-MONOMER. CPNE115713:CPN0081-MONOMER. CPNE138677:CPJ0081-MONOMER. CPNE182082:CPB0081-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_CHLPN

Accession

Primary (citable) accession number: Q9Z9A0
Secondary accession number(s): Q9JSJ8, Q9K210

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program