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Protein

Pyruvate kinase

Gene

pyk

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathway:iglycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331SubstrateBy similarity
Metal bindingi35 – 351PotassiumBy similarity
Metal bindingi37 – 371PotassiumBy similarity
Metal bindingi67 – 671PotassiumBy similarity
Metal bindingi68 – 681Potassium; via carbonyl oxygenBy similarity
Sitei219 – 2191Transition state stabilizerBy similarity
Metal bindingi221 – 2211MagnesiumBy similarity
Binding sitei244 – 2441Substrate; via amide nitrogenBy similarity
Metal bindingi245 – 2451MagnesiumBy similarity
Binding sitei245 – 2451Substrate; via amide nitrogenBy similarity
Binding sitei277 – 2771SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-699-MONOMER.
CPNE115713:GHEY-100-MONOMER.
CPNE138677:GH8N-100-MONOMER.
CPNE182082:GH4N-100-MONOMER.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:CPn_0097, CP_0677, CpB0097
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000583 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Pyruvate kinasePRO_0000112062Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi115713.CPn0097.

Structurei

3D structure databases

ProteinModelPortaliQ9Z984.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
KOiK00873.
OMAiVLCGMMT.
OrthoDBiEOG6GBMB0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z984-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITRTKIICT IGPATNSPEM LAKLLDAGMN VARLNFSHGS HETHGQAIGF
60 70 80 90 100
LKELREQKRV PLAIMLDTKG PEIRLGNIPQ PISVSQGQKL RLVSSDIDGS
110 120 130 140 150
AEGGVSLYPK GIFPFVPEGA DVLIDDGYIH AVVVSSEADS LELEFMNSGL
160 170 180 190 200
LKSHKSLSIR GVDVALPFMT EKDIADLKFG VEQNMDVVAA SFVRYGEDIE
210 220 230 240 250
TMRKCLADLG NPKMPIIAKI ENRLGVENFS KIAKLADGIM IARGDLGIEL
260 270 280 290 300
SVVEVPNLQK MMAKVSRETG HFCVTATQML ESMIRNVLPT RAEVSDIANA
310 320 330 340 350
IYDGSSAVML SGETASGAHP VAAVKIMRSV ILETEKNLSH DSFLKLDDSN
360 370 380 390 400
SALQVSPYLS AIGLAGIQIA ERADAKALIV YTESGSSPMF LSKYRPKFPI
410 420 430 440 450
IAVTPSTSVY YRLALEWGVY PMLTQESDRA VWRHQACIYG IEQGILSNYD
460 470 480
RILVLSRGAC MEETNNLTLT IVNDILTGSE FPET
Length:484
Mass (Da):52,666
Last modified:May 1, 1999 - v1
Checksum:i101E49E4F7CDD7B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881V → N in BAA98307 (PubMed:10871362).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18250.1.
AE002161 Genomic DNA. Translation: AAF38488.1.
BA000008 Genomic DNA. Translation: BAA98307.1.
AE009440 Genomic DNA. Translation: AAP98030.1.
PIRiA72119.
A86503.
RefSeqiNP_224305.1. NC_000922.1.

Genome annotation databases

EnsemblBacteriaiAAD18250; AAD18250; CPn_0097.
AAF38488; AAF38488; CP_0677.
AAP98030; AAP98030; CpB0097.
BAA98307; BAA98307; BAA98307.
GeneIDi895703.
KEGGicpa:CP0677.
cpj:CPj0097.
cpn:CPn0097.
cpt:CpB0097.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18250.1.
AE002161 Genomic DNA. Translation: AAF38488.1.
BA000008 Genomic DNA. Translation: BAA98307.1.
AE009440 Genomic DNA. Translation: AAP98030.1.
PIRiA72119.
A86503.
RefSeqiNP_224305.1. NC_000922.1.

3D structure databases

ProteinModelPortaliQ9Z984.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi115713.CPn0097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD18250; AAD18250; CPn_0097.
AAF38488; AAF38488; CP_0677.
AAP98030; AAP98030; CpB0097.
BAA98307; BAA98307; BAA98307.
GeneIDi895703.
KEGGicpa:CP0677.
cpj:CPj0097.
cpn:CPn0097.
cpt:CpB0097.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
KOiK00873.
OMAiVLCGMMT.
OrthoDBiEOG6GBMB0.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.
BioCyciCPNE115711:GI7B-699-MONOMER.
CPNE115713:GHEY-100-MONOMER.
CPNE138677:GH8N-100-MONOMER.
CPNE182082:GH4N-100-MONOMER.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiKPYK_CHLPN
AccessioniPrimary (citable) accession number: Q9Z984
Secondary accession number(s): Q9JSJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.