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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-687-MONOMER.
CPNE115713:GHEY-113-MONOMER.
CPNE138677:GH8N-112-MONOMER.
CPNE182082:GH4N-113-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CPn_0109, CP_0665, CpB0110
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000583 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10431043Isoleucine--tRNA ligasePRO_0000098528Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi115713.CPn0109.

Structurei

3D structure databases

ProteinModelPortaliQ9Z972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTADEVGKNS FAKKEEQVLK FWKDNQIFEK SLQNRQGKTL YSFYDGPPFA
60 70 80 90 100
TGLPHYGHLL ASTIKDVVGR YATMDGYYVP RRFGWDCHGV PVEYEVEKSL
110 120 130 140 150
SLTAPGAIED FGIASFNEEC RKIVFRYVHE WEYYINRIGR WVDFSSTWKT
160 170 180 190 200
MDASFMESVW WVFQSLYNQG LVYEGTKVVP FSTALGTPLS NFEASQNYKE
210 220 230 240 250
VDDPSLVVRM PLQNDSASLL VWTTTPWTLP SNMAIAVGET LVYVRIQDKK
260 270 280 290 300
SGEQWILSQG CVSRWFSNPE EFVILESFSG KDLVGRTYEP PFTFFQSKRE
310 320 330 340 350
EGAFRVIAAS FVEESEGTGV VHMAPAFGEG DFLVCKENHV PLVCPVDAHG
360 370 380 390 400
SFTEEIPQYQ GQYIKHADKE IIKFLKKEGR IFYHGTVKHR YPFCWRTDTP
410 420 430 440 450
LIYKAVNSWF VAVEKIKDKM LRANSSIHWV PEHIQEGRFG KWLEGARDWA
460 470 480 490 500
ISRNRYWGTP IPIWKSADGE ILVVGSIREL EELTGTQITD IHRHFIDDLN
510 520 530 540 550
IVKDGKPFHR IPYVFDCWFD SGAMPYAQNH YPFENQKETE EAFPADFIAE
560 570 580 590 600
GLDQTRGWFY TLTVISAILF DRPAFRNAIV NGIILAEDGN KMSKRLNNYP
610 620 630 640 650
SPKYVLDTYG ADALRLYLLH SVVVKAEDLR FSDKGIEGVL KQILLPLTNV
660 670 680 690 700
LSFFNTYAEL YGFDPKSQDI EPAYTEIDQW ILSNLYSVVG KVRESMSQYH
710 720 730 740 750
LNFAVEPFVT FIDDLTNWYI RRCRRRFWEA EDTPDRRAAF STLYEVLTVF
760 770 780 790 800
CKVIAPFVPF LAEDIYQKLK LEKEPESVHL CDFPQVEMDK ILPDLEKRMH
810 820 830 840 850
DIREIVGLGH SLRKEHKLKV RQPLANFYVV GSKDRLSLLK TFEGLIAEEL
860 870 880 890 900
NVKNVIFYEE APSFIYTTVK PNFRMLGKKV GSKMKEVQKA LSELPNNAID
910 920 930 940 950
KLIQEETWVL TIDDREIALD GDDVVICRHT DPGYIARSSA LFSVILDCQL
960 970 980 990 1000
REPLIVEGIA RELVNKINTM RRNQQLHVSD RIALRIKTTE AVHRAFLDYE
1010 1020 1030 1040
NYICEETLII AYDFTQDSDF QGENWDINGH ATQIEITVSS IDS
Length:1,043
Mass (Da):120,287
Last modified:May 1, 1999 - v1
Checksum:i08ABB8B9364E640C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti384 – 3841H → Y in AAF38477 (PubMed:10684935).Curated
Sequence conflicti798 – 7981R → H in AAP98043 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18262.1.
AE002161 Genomic DNA. Translation: AAF38477.1.
BA000008 Genomic DNA. Translation: BAA98320.1.
AE009440 Genomic DNA. Translation: AAP98043.1.
PIRiC81552.
E72118.
F86504.
RefSeqiNP_224317.1. NC_000922.1.
NP_300169.1. NC_002491.1.
NP_445207.1. NC_002179.2.
NP_876386.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD18262; AAD18262; CPn_0109.
AAF38477; AAF38477; CP_0665.
AAP98043; AAP98043; CpB0110.
BAA98320; BAA98320; BAA98320.
GeneIDi894633.
KEGGicpa:CP0665.
cpj:CPj0109.
cpn:CPn0109.
cpt:CpB0110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18262.1.
AE002161 Genomic DNA. Translation: AAF38477.1.
BA000008 Genomic DNA. Translation: BAA98320.1.
AE009440 Genomic DNA. Translation: AAP98043.1.
PIRiC81552.
E72118.
F86504.
RefSeqiNP_224317.1. NC_000922.1.
NP_300169.1. NC_002491.1.
NP_445207.1. NC_002179.2.
NP_876386.1. NC_005043.1.

3D structure databases

ProteinModelPortaliQ9Z972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi115713.CPn0109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD18262; AAD18262; CPn_0109.
AAF38477; AAF38477; CP_0665.
AAP98043; AAP98043; CpB0110.
BAA98320; BAA98320; BAA98320.
GeneIDi894633.
KEGGicpa:CP0665.
cpj:CPj0109.
cpn:CPn0109.
cpt:CpB0110.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-687-MONOMER.
CPNE115713:GHEY-113-MONOMER.
CPNE138677:GH8N-112-MONOMER.
CPNE182082:GH4N-113-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiSYI_CHLPN
AccessioniPrimary (citable) accession number: Q9Z972
Secondary accession number(s): Q9JQK2, Q9K221
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: June 24, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.