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Q9Z972

- SYI_CHLPN

UniProt

Q9Z972 - SYI_CHLPN

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-687-MONOMER.
CPNE115713:GHEY-113-MONOMER.
CPNE138677:GH8N-112-MONOMER.
CPNE182082:GH4N-113-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CPn_0109, CP_0665, CpB0110
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000424: Chromosome, UP000000583: Chromosome, UP000000801: Chromosome, UP000000802: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10431043Isoleucine--tRNA ligasePRO_0000098528Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi115713.CPn0109.

Structurei

3D structure databases

ProteinModelPortaliQ9Z972.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z972-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTADEVGKNS FAKKEEQVLK FWKDNQIFEK SLQNRQGKTL YSFYDGPPFA
60 70 80 90 100
TGLPHYGHLL ASTIKDVVGR YATMDGYYVP RRFGWDCHGV PVEYEVEKSL
110 120 130 140 150
SLTAPGAIED FGIASFNEEC RKIVFRYVHE WEYYINRIGR WVDFSSTWKT
160 170 180 190 200
MDASFMESVW WVFQSLYNQG LVYEGTKVVP FSTALGTPLS NFEASQNYKE
210 220 230 240 250
VDDPSLVVRM PLQNDSASLL VWTTTPWTLP SNMAIAVGET LVYVRIQDKK
260 270 280 290 300
SGEQWILSQG CVSRWFSNPE EFVILESFSG KDLVGRTYEP PFTFFQSKRE
310 320 330 340 350
EGAFRVIAAS FVEESEGTGV VHMAPAFGEG DFLVCKENHV PLVCPVDAHG
360 370 380 390 400
SFTEEIPQYQ GQYIKHADKE IIKFLKKEGR IFYHGTVKHR YPFCWRTDTP
410 420 430 440 450
LIYKAVNSWF VAVEKIKDKM LRANSSIHWV PEHIQEGRFG KWLEGARDWA
460 470 480 490 500
ISRNRYWGTP IPIWKSADGE ILVVGSIREL EELTGTQITD IHRHFIDDLN
510 520 530 540 550
IVKDGKPFHR IPYVFDCWFD SGAMPYAQNH YPFENQKETE EAFPADFIAE
560 570 580 590 600
GLDQTRGWFY TLTVISAILF DRPAFRNAIV NGIILAEDGN KMSKRLNNYP
610 620 630 640 650
SPKYVLDTYG ADALRLYLLH SVVVKAEDLR FSDKGIEGVL KQILLPLTNV
660 670 680 690 700
LSFFNTYAEL YGFDPKSQDI EPAYTEIDQW ILSNLYSVVG KVRESMSQYH
710 720 730 740 750
LNFAVEPFVT FIDDLTNWYI RRCRRRFWEA EDTPDRRAAF STLYEVLTVF
760 770 780 790 800
CKVIAPFVPF LAEDIYQKLK LEKEPESVHL CDFPQVEMDK ILPDLEKRMH
810 820 830 840 850
DIREIVGLGH SLRKEHKLKV RQPLANFYVV GSKDRLSLLK TFEGLIAEEL
860 870 880 890 900
NVKNVIFYEE APSFIYTTVK PNFRMLGKKV GSKMKEVQKA LSELPNNAID
910 920 930 940 950
KLIQEETWVL TIDDREIALD GDDVVICRHT DPGYIARSSA LFSVILDCQL
960 970 980 990 1000
REPLIVEGIA RELVNKINTM RRNQQLHVSD RIALRIKTTE AVHRAFLDYE
1010 1020 1030 1040
NYICEETLII AYDFTQDSDF QGENWDINGH ATQIEITVSS IDS
Length:1,043
Mass (Da):120,287
Last modified:May 1, 1999 - v1
Checksum:i08ABB8B9364E640C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti384 – 3841H → Y in AAF38477. (PubMed:10684935)Curated
Sequence conflicti798 – 7981R → H in AAP98043. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18262.1.
AE002161 Genomic DNA. Translation: AAF38477.1.
BA000008 Genomic DNA. Translation: BAA98320.1.
AE009440 Genomic DNA. Translation: AAP98043.1.
PIRiC81552.
E72118.
F86504.
RefSeqiNP_224317.1. NC_000922.1.
NP_300169.1. NC_002491.1.
NP_445207.1. NC_002179.2.
NP_876386.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD18262; AAD18262; CPn_0109.
AAF38477; AAF38477; CP_0665.
AAP98043; AAP98043; CpB0110.
BAA98320; BAA98320; BAA98320.
GeneIDi1466793.
894633.
918769.
963852.
KEGGicpa:CP0665.
cpj:CPj0109.
cpn:CPn0109.
cpt:CpB0110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18262.1 .
AE002161 Genomic DNA. Translation: AAF38477.1 .
BA000008 Genomic DNA. Translation: BAA98320.1 .
AE009440 Genomic DNA. Translation: AAP98043.1 .
PIRi C81552.
E72118.
F86504.
RefSeqi NP_224317.1. NC_000922.1.
NP_300169.1. NC_002491.1.
NP_445207.1. NC_002179.2.
NP_876386.1. NC_005043.1.

3D structure databases

ProteinModelPortali Q9Z972.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 115713.CPn0109.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD18262 ; AAD18262 ; CPn_0109 .
AAF38477 ; AAF38477 ; CP_0665 .
AAP98043 ; AAP98043 ; CpB0110 .
BAA98320 ; BAA98320 ; BAA98320 .
GeneIDi 1466793.
894633.
918769.
963852.
KEGGi cpa:CP0665.
cpj:CPj0109.
cpn:CPn0109.
cpt:CpB0110.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CPNE115711:GI7B-687-MONOMER.
CPNE115713:GHEY-113-MONOMER.
CPNE138677:GH8N-112-MONOMER.
CPNE182082:GH4N-113-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiSYI_CHLPN
AccessioniPrimary (citable) accession number: Q9Z972
Secondary accession number(s): Q9JQK2, Q9K221
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3