Q9Z972 (SYI_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	CPn_0109, CP_0665, CpB0110

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	1043 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003
Cofactor	Zinc By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1043	1043	Isoleucine--tRNA ligase HAMAP-Rule MF_02003		PRO_0000098528
Regions
	Motif	48 – 58	11	"HIGH" region HAMAP-Rule MF_02003
	Motif	591 – 595	5	"KMSKS" region HAMAP-Rule MF_02003
Sites
	Binding site	594	1	ATP By similarity
Experimental info
	Sequence conflict	384	1	H → Y in AAF38477. Ref.2
	Sequence conflict	798	1	R → H in AAP98043. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9Z972 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 08ABB8B9364E640C	FASTA	1,043	120,287
	10 20 30 40 50 60 MTADEVGKNS FAKKEEQVLK FWKDNQIFEK SLQNRQGKTL YSFYDGPPFA TGLPHYGHLL 70 80 90 100 110 120 ASTIKDVVGR YATMDGYYVP RRFGWDCHGV PVEYEVEKSL SLTAPGAIED FGIASFNEEC 130 140 150 160 170 180 RKIVFRYVHE WEYYINRIGR WVDFSSTWKT MDASFMESVW WVFQSLYNQG LVYEGTKVVP 190 200 210 220 230 240 FSTALGTPLS NFEASQNYKE VDDPSLVVRM PLQNDSASLL VWTTTPWTLP SNMAIAVGET 250 260 270 280 290 300 LVYVRIQDKK SGEQWILSQG CVSRWFSNPE EFVILESFSG KDLVGRTYEP PFTFFQSKRE 310 320 330 340 350 360 EGAFRVIAAS FVEESEGTGV VHMAPAFGEG DFLVCKENHV PLVCPVDAHG SFTEEIPQYQ 370 380 390 400 410 420 GQYIKHADKE IIKFLKKEGR IFYHGTVKHR YPFCWRTDTP LIYKAVNSWF VAVEKIKDKM 430 440 450 460 470 480 LRANSSIHWV PEHIQEGRFG KWLEGARDWA ISRNRYWGTP IPIWKSADGE ILVVGSIREL 490 500 510 520 530 540 EELTGTQITD IHRHFIDDLN IVKDGKPFHR IPYVFDCWFD SGAMPYAQNH YPFENQKETE 550 560 570 580 590 600 EAFPADFIAE GLDQTRGWFY TLTVISAILF DRPAFRNAIV NGIILAEDGN KMSKRLNNYP 610 620 630 640 650 660 SPKYVLDTYG ADALRLYLLH SVVVKAEDLR FSDKGIEGVL KQILLPLTNV LSFFNTYAEL 670 680 690 700 710 720 YGFDPKSQDI EPAYTEIDQW ILSNLYSVVG KVRESMSQYH LNFAVEPFVT FIDDLTNWYI 730 740 750 760 770 780 RRCRRRFWEA EDTPDRRAAF STLYEVLTVF CKVIAPFVPF LAEDIYQKLK LEKEPESVHL 790 800 810 820 830 840 CDFPQVEMDK ILPDLEKRMH DIREIVGLGH SLRKEHKLKV RQPLANFYVV GSKDRLSLLK 850 860 870 880 890 900 TFEGLIAEEL NVKNVIFYEE APSFIYTTVK PNFRMLGKKV GSKMKEVQKA LSELPNNAID 910 920 930 940 950 960 KLIQEETWVL TIDDREIALD GDDVVICRHT DPGYIARSSA LFSVILDCQL REPLIVEGIA 970 980 990 1000 1010 1020 RELVNKINTM RRNQQLHVSD RIALRIKTTE AVHRAFLDYE NYICEETLII AYDFTQDSDF 1030 1040 QGENWDINGH ATQIEITVSS IDS

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18262.1. AE002161 Genomic DNA. Translation: AAF38477.1. BA000008 Genomic DNA. Translation: BAA98320.1. AE009440 Genomic DNA. Translation: AAP98043.1.
PIR	C81552. E72118. F86504.
RefSeq	NP_224317.1. NC_000922.1. NP_300169.1. NC_002491.1. NP_445207.1. NC_002179.2. NP_876386.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z972.
ModBase	Search...
Protein-protein interaction databases
STRING	115713.CPn0109.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD18262; AAD18262; CPn_0109. AAF38477; AAF38477; CP_0665. AAP98043; AAP98043; CpB0110. BAA98320; BAA98320; BAA98320.
GeneID	1466793. 894633. 918769. 963852.
KEGG	cpa:CP0665. cpj:CPj0109. cpn:CPn0109. cpt:CpB0110.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246403.
KO	K01870.
OMA	SRSRYWG.
ProtClustDB	PRK06039.
Enzyme and pathway databases
BioCyc	CPNE115711:GI7B-663-MONOMER. CPNE115713:GHEY-112-MONOMER. CPNE138677:GH8N-106-MONOMER. CPNE182082:GH4N-111-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02003. Ile_tRNA_synth_type2.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_CHLPN

Accession

Primary (citable) accession number: Q9Z972
Secondary accession number(s): Q9JQK2, Q9K221

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program