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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei594ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CPn_0109, CP_0665, CpB0110
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000583 Componenti: Chromosome
  • UP000000801 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985281 – 1043Isoleucine--tRNA ligaseAdd BLAST1043

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi182082.CpB0110

Structurei

3D structure databases

ProteinModelPortaliQ9Z972
SMRiQ9Z972
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 58"HIGH" regionAdd BLAST11
Motifi591 – 595"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTADEVGKNS FAKKEEQVLK FWKDNQIFEK SLQNRQGKTL YSFYDGPPFA
60 70 80 90 100
TGLPHYGHLL ASTIKDVVGR YATMDGYYVP RRFGWDCHGV PVEYEVEKSL
110 120 130 140 150
SLTAPGAIED FGIASFNEEC RKIVFRYVHE WEYYINRIGR WVDFSSTWKT
160 170 180 190 200
MDASFMESVW WVFQSLYNQG LVYEGTKVVP FSTALGTPLS NFEASQNYKE
210 220 230 240 250
VDDPSLVVRM PLQNDSASLL VWTTTPWTLP SNMAIAVGET LVYVRIQDKK
260 270 280 290 300
SGEQWILSQG CVSRWFSNPE EFVILESFSG KDLVGRTYEP PFTFFQSKRE
310 320 330 340 350
EGAFRVIAAS FVEESEGTGV VHMAPAFGEG DFLVCKENHV PLVCPVDAHG
360 370 380 390 400
SFTEEIPQYQ GQYIKHADKE IIKFLKKEGR IFYHGTVKHR YPFCWRTDTP
410 420 430 440 450
LIYKAVNSWF VAVEKIKDKM LRANSSIHWV PEHIQEGRFG KWLEGARDWA
460 470 480 490 500
ISRNRYWGTP IPIWKSADGE ILVVGSIREL EELTGTQITD IHRHFIDDLN
510 520 530 540 550
IVKDGKPFHR IPYVFDCWFD SGAMPYAQNH YPFENQKETE EAFPADFIAE
560 570 580 590 600
GLDQTRGWFY TLTVISAILF DRPAFRNAIV NGIILAEDGN KMSKRLNNYP
610 620 630 640 650
SPKYVLDTYG ADALRLYLLH SVVVKAEDLR FSDKGIEGVL KQILLPLTNV
660 670 680 690 700
LSFFNTYAEL YGFDPKSQDI EPAYTEIDQW ILSNLYSVVG KVRESMSQYH
710 720 730 740 750
LNFAVEPFVT FIDDLTNWYI RRCRRRFWEA EDTPDRRAAF STLYEVLTVF
760 770 780 790 800
CKVIAPFVPF LAEDIYQKLK LEKEPESVHL CDFPQVEMDK ILPDLEKRMH
810 820 830 840 850
DIREIVGLGH SLRKEHKLKV RQPLANFYVV GSKDRLSLLK TFEGLIAEEL
860 870 880 890 900
NVKNVIFYEE APSFIYTTVK PNFRMLGKKV GSKMKEVQKA LSELPNNAID
910 920 930 940 950
KLIQEETWVL TIDDREIALD GDDVVICRHT DPGYIARSSA LFSVILDCQL
960 970 980 990 1000
REPLIVEGIA RELVNKINTM RRNQQLHVSD RIALRIKTTE AVHRAFLDYE
1010 1020 1030 1040
NYICEETLII AYDFTQDSDF QGENWDINGH ATQIEITVSS IDS
Length:1,043
Mass (Da):120,287
Last modified:May 1, 1999 - v1
Checksum:i08ABB8B9364E640C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti384H → Y in AAF38477 (PubMed:10684935).Curated1
Sequence conflicti798R → H in AAP98043 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA Translation: AAD18262.1
AE002161 Genomic DNA Translation: AAF38477.1
BA000008 Genomic DNA Translation: BAA98320.1
AE009440 Genomic DNA Translation: AAP98043.1
PIRiC81552
E72118
F86504
RefSeqiNP_224317.1, NC_000922.1
WP_010882759.1, NZ_LN847257.1

Genome annotation databases

EnsemblBacteriaiAAD18262; AAD18262; CPn_0109
AAF38477; AAF38477; CP_0665
AAP98043; AAP98043; CpB0110
BAA98320; BAA98320; BAA98320
GeneIDi894633
KEGGicpa:CP_0665
cpj:ileS
cpn:CPn0109
cpt:CpB0110
PATRICifig|115713.3.peg.123

Similar proteinsi

Entry informationi

Entry nameiSYI_CHLPN
AccessioniPrimary (citable) accession number: Q9Z972
Secondary accession number(s): Q9JQK2, Q9K221
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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