Q9Z8U9 (KDSB_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferase
EC=2.7.7.38

Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthase
Short name=CKS
Short name=CMP-KDO synthase

Gene names

Name:	kdsB
Ordered Locus Names:	CPn_0235, CP_0527, CpB0241

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity. HAMAP MF_00057
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP MF_00057 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00057
Subcellular location	Cytoplasm Potential HAMAP MF_00057.
Sequence similarities	Belongs to the KdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	3-deoxy-manno-octulosonate cytidylyltransferase HAMAP MF_00057		PRO_0000188499

Sequences

	Sequence		Length	Mass (Da)
	Q9Z8U9 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 8A4B2B573EDF405B	FASTA	254	28,414
	10 20 30 40 50 60 MKPEESECLC IGVLPARWNS SRYPGKPLAK IHGKSLIQRT YENASQSSLL DKIVVATDDQ 70 80 90 100 110 120 HIIDHVTDFG GYAVMTSPTC SNGTERTGEV ARKYFPKAEI IVNIQGDEPC LNSEVVDALV 130 140 150 160 170 180 QKLRSSPEAE LVTPVALTTD REEILTEKKV KCVFDSEGRA LYFSRSPIPF ILKKATPVYL 190 200 210 220 230 240 HIGVYAFKRE ALFRYLQHSS TPLSDAEDLE QLRFLEHGGK IHVCIVDAKS PSVDYPEDIA 250 KVEQYITCLS NAYF

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18388.1. AE002161 Genomic DNA. Translation: AAF38351.1. BA000008 Genomic DNA. Translation: BAA98445.1. AE009440 Genomic DNA. Translation: AAP98174.1.
PIR	C86520. H72102.
RefSeq	NP_224444.1. NC_000922.1. NP_300294.1. NC_002491.1. NP_445071.1. NC_002179.2. NP_876517.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z8U9.
ModBase	Search...
2D gel databases
PHCI-2DPAGE	Q9Z8U9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1466924. 895121. 919018. 963770.
GenomeReviews	Gene locus CPn_0235 in contig AE001363_GR. Gene locus CP_0527 in contig AE002161_GR. Gene locus CpB0241 in contig AE009440_GR. Gene locus kdsB in contig BA000008_GR.
KEGG	cpa:CP0527. cpn:CPn0235. cpt:CpB0241.
TIGR	CP_0527.
Phylogenomic databases
HOGENOM	HBG637773.
OMA	INVQADE.
ProtClustDB	PRK05450.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0527-MONOMER. CPNE115713:CPN0235-MONOMER. CPNE138677:CPJ0235-MONOMER. CPNE182082:CPB0241-MONOMER.
Family and domain databases
HAMAP	MF_00057. CMP-KDO_synth. [Tree]
InterPro	IPR003329. Cytidylyl_trans. IPR004528. KdsB. [Graphical view]
KO	K00979.
PANTHER	PTHR21485:SF4. PTHR21485:SF4. 1 hit.
Pfam	PF02348. CTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00466. KdsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSB_CHLPN

Accession

Primary (citable) accession number: Q9Z8U9
Secondary accession number(s): Q9JQ61

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program