Q9Z8R4 (GYRA_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	CPn_0274, CP_0485, CpB0281

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145229
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	232	1	T → A in AAD18423. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Z8R4 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: A2C43C31A6C7B19C	FASTA	834	93,839
	10 20 30 40 50 60 MFNKDEIIVP KNLEEEMKES YLRYSMSVII SRALPDIRDG LKPSQRRVLY AMKQLSLSPG 70 80 90 100 110 120 AKHRKCAKIC GDTSGDYHPH GESVIYPTLV RMAQNWAMRY PLVDGQGNFG SIDGDPPAAM 130 140 150 160 170 180 RYTEARLTHS AMYLMEDLDK DTVDIVPNYD ETKHEPVVFP SKFPNLLCNG SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLGEL IEATLLLLAN PQASVDEILQ VMPGPDFPTG GIICGSEGIR STYTTGRGKI 250 260 270 280 290 300 KVRARLHVEE NEDKHRESII ITEMPYNVNK SRLIEQIANL VNEKTLAGIS DVRDESDKDG 310 320 330 340 350 360 IRVVLEIKKG ESSEIIINRL YKFTDVQVTF GANMLALDKN LPRTMSIHRM ISAWIRHRKE 370 380 390 400 410 420 VIRRRTRYEL NKAETRAHVL EGYLKALSCL DALVKTIRES GNKEHAKERI IESFGFTEPQ 430 440 450 460 470 480 ALAILELRLY QLTGLEAEKI QKEYEELLNK IAYYKQVLSD EGLVKDIIRN ELQDLLKHHK 490 500 510 520 530 540 VARRTTIEFD ADDIRDIEDI ITNESVIITI SGDDYVKRMP VKVFKEQRRG GHGVTGFDMK 550 560 570 580 590 600 KGAGFLKAVY SAFTKDYLLI FTNFGQCYWL KVWQLPEGER RAKGKPIINF LEGIRPGEEL 610 620 630 640 650 660 AAILNIKNFD NAGFLFLATK RGVVKKVSLD AFSNPRKKGI RALEIDEGDE LIAACHIVSD 670 680 690 700 710 720 EEKVMLFTHL GMAVRFPHEK VRPMGRTARG VRGVSLKNEE DKVVSCQIVT ENQSVLIVCD 730 740 750 760 770 780 QGFGKRSLVE DFRETNRGGV GVRSILINER NGNVLGAIPV TDHDSILLMS SQGQAIRINM 790 800 810 820 830 QDVRVMGRST QGVRLVHLKE GDALVSMEKL SSNENDDEVL SGSEEECSDT VSLR

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18423.1. AE002161 Genomic DNA. Translation: AAF38315.1. BA000008 Genomic DNA. Translation: BAA98484.1. AE009440 Genomic DNA. Translation: AAP98214.1.
PIR	B86525. F81571. G72098.
RefSeq	NP_224479.1. NC_000922.1. NP_300333.1. NC_002491.1. NP_445032.1. NC_002179.2. NP_876557.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z8R4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1466964. 894973. 919058. 963490.
GenomeReviews	Gene locus CPn_0274 in contig AE001363_GR. Gene locus CP_0485 in contig AE002161_GR. Gene locus CpB0281 in contig AE009440_GR. Gene locus gyrA in contig BA000008_GR.
KEGG	cpa:CP0485. cpn:CPn0274. cpt:CpB0281.
TIGR	CP_0485.
Phylogenomic databases
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0485-MONOMER. CPNE115713:CPN0274-MONOMER. CPNE138677:CPJ0274-MONOMER. CPNE182082:CPB0281-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_CHLPN

Accession

Primary (citable) accession number: Q9Z8R4
Secondary accession number(s): Q9JRY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 11, 2001
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program