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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei178UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei186UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei380Meso-diaminopimelateUniRule annotation1
Binding sitei453Meso-diaminopimelate; via carbonyl oxygenUniRule annotation1
Binding sitei457Meso-diaminopimelateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi109 – 115ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotation
Ordered Locus Names:CPn_0418, CP_0336, CpB0434
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000583 Componenti: Chromosome
  • UP000000801 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001018811 – 483UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseAdd BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi182082.CpB0434

Structurei

3D structure databases

ProteinModelPortaliQ9Z8C5
SMRiQ9Z8C5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 152UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation2
Regioni403 – 406Meso-diaminopimelate bindingUniRule annotation4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi403 – 406Meso-diaminopimelate recognition motif4

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EEN Bacteria
COG0769 LUCA
HOGENOMiHOG000268118
KOiK01928
OMAiCFMEVSS
OrthoDBiPOG091H0082

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00208 MurE, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR000713 Mur_ligase_N
IPR035911 MurE/MurF_N
IPR005761 UDP-N-AcMur-Glu-dNH2Pim_ligase
PfamiView protein in Pfam
PF01225 Mur_ligase, 1 hit
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
SSF63418 SSF63418, 1 hit
TIGRFAMsiTIGR01085 murE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z8C5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLKELLHGV QAKIYGKVRP LEVRNLTRDS RCVSVGDIFI AHKGQRYDGN
60 70 80 90 100
DFAVDALANG AIAIASSLYN PFLSVVQIIT PNLEELEAEL SAKYYEYPSS
110 120 130 140 150
KLHTIGVTGT NGKTTVTCLI KALLDSYQKP SGLLGTIEHI LGEGVIKDGF
160 170 180 190 200
TTPTPALLQK YLATMVRQNR DAVVMEVSSI GLASGRVAYT NFDTAVLTNI
210 220 230 240 250
TLDHLDFHGT FETYVAAKAK LFSLVPPSGM VVINTDSPYA SQCIESAKAP
260 270 280 290 300
VITYGIESAA DYRATDIQLS SSGTKYTLVY GDQKIACSSS FIGKYNVYNL
310 320 330 340 350
LAAISTVHAS LRCDLEDLLE KIGLCQPPPG RLDPVLMGPC PVYIDYAHTP
360 370 380 390 400
DALDNVLTGL HELLPEGGRL IVVFGCGGDR DRSKRKLMAQ VVERYGFAVV
410 420 430 440 450
TSDNPRSEPP EDIVNEICDG FYSKNYFIEI DRKQAITYAL SIASDRDIVL
460 470 480
IAGKGHEAYQ IFKHQTVAFD DKQTVCEVLA SYV
Length:483
Mass (Da):52,720
Last modified:May 1, 1999 - v1
Checksum:i3A0FEEA93EDD76ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA Translation: AAD18562.1
AE002161 Genomic DNA Translation: AAF38190.1
BA000008 Genomic DNA Translation: BAA98626.1
AE009440 Genomic DNA Translation: AAP98365.1
PIRiD72080
H86542
RefSeqiNP_224618.1, NC_000922.1
WP_010883061.1, NZ_LN847257.1

Genome annotation databases

EnsemblBacteriaiAAD18562; AAD18562; CPn_0418
AAF38190; AAF38190; CP_0336
AAP98365; AAP98365; CpB0434
BAA98626; BAA98626; BAA98626
GeneIDi894904
KEGGicpa:CP_0336
cpj:murE
cpn:CPn0418
cpt:CpB0434
PATRICifig|115713.3.peg.462

Similar proteinsi

Entry informationi

Entry nameiMURE_CHLPN
AccessioniPrimary (citable) accession number: Q9Z8C5
Secondary accession number(s): Q9JQA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health