Reviewed, UniProtKB/Swiss-Prot Q9Z876 (GLGB_CHLPN)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	CPn_0475, CP_0279, CpB0494

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188693
Sites
	Active site	295	1	By similarity
	Active site	330	1	By similarity
	Active site	335	1	By similarity
	Active site	398	1	By similarity
	Active site	400	1	By similarity
	Active site	453	1	By similarity
	Active site	520	1	By similarity
	Active site	521	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z876-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: CCF5FD309971C87B	FASTA	720	82,839
	10 20 30 40 50 60 MVDKLIHPWD LDLLVSGRQK DPHKLLGILA SEDSSDHIVI FRPGAHTVAI ELLGELHHAV 70 80 90 100 110 120 AYRSGLFFLS VPKGIGHGDY RVYHQNGLLA HDPYAFPPLW GEIDSFLFHR GTHYRIYERM 130 140 150 160 170 180 GAIPMEVQGI SGVLFVLWAP HAQRVSVVGD FNFWHGLVNP LRKISDQGIW ELFVPGLGEG 190 200 210 220 230 240 IRYKWEIVTQ SGNVIVKTDP YGKSFDPPPQ GTARVADSES YSWSDHRWME RRSKQSEGPV 250 260 270 280 290 300 TIYEVHLGSW QWQEGRPLSY SEMAHRLASY CKEMHYTHVE LLPITEHPLN ESWGYQVTGY 310 320 330 340 350 360 YAPTSRYGTL QEFQYFVDYL HKENIGIILD WVPGHFPVDA FALASFDGEP LYEYTGHSQA 370 380 390 400 410 420 LHPHWNTFTF DYSRHEVTNF LLGSALFWLD KMHIDGLRVD AVASMLYRDY GREDGEWTPN 430 440 450 460 470 480 IYGGKENLES IEFLKHLNSV IHKEFSGVLT FAEESTAFPG VTKDVDQGGL GFDYKWNLGW 490 500 510 520 530 540 MHDTFHYFMK DPMYRKYHQK DLTFSLWYAF QESFILPLSH DEVVHGKGSL VNKLPGDTWT 550 560 570 580 590 600 RFAQMRVLLS YQICLPGKKL LFMGGEFGQY GEWSPDRPLD WELLNHHYHK TLRNCVSALN 610 620 630 640 650 660 ALYIHQPYLW MQESSQECFH WVDFHDIENN VIAYYRFAGS NRSSALLCVH HFSASTFPSY 670 680 690 700 710 720 VLRCEGVKHC ELLLNTDDES FGGSGKGNRA PVVCQDQGVA WGLDIELPPL ATVIYLVTFF

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001363 Genomic DNA. Translation: AAD18615.1. AE002161 Genomic DNA. Translation: AAF38137.1. BA000008 Genomic DNA. Translation: BAA98681.1. AE009440 Genomic DNA. Translation: AAP98425.1.
PIR	E72074. G86549.
RefSeq	NP_224671.1. NP_300530.1. NP_444829.1. NP_876768.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	1467175. 895645. 919217. 963361.
GenomeReviews	Gene locus CPn_0475 in contig AE001363_GR. Gene locus CP_0279 in contig AE002161_GR. Gene locus CpB0494 in contig AE009440_GR. Gene locus glgB in contig BA000008_GR.
KEGG	cpa:CP0279. cpn:CPn0475. cpt:CpB0494.
TIGR	CP_0279.
Phylogenomic databases
HOGENOM	Q9Z876.
OMA	PKTASIV.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0279-MON. CPNE115713:CPN0475-MON. CPNE138677:CPJ0475-MON. CPNE182082:CPB0494-MON.
BRENDA	2.4.1.18. 264832.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_CHLPN

Accession

Primary (citable) accession number: Q9Z876

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)