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Q9Z833 (DAPF_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diaminopimelate epimerase

Short name=DAP epimerase
EC=5.1.1.7
Gene names
Name:dapF
Ordered Locus Names:CPn_0519, CP_0234, CpB0540
OrganismChlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]
Taxonomic identifier83558 [NCBI]
Taxonomic lineageBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity. HAMAP-Rule MF_00197

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00197.

Sequence similarities

Belongs to the diaminopimelate epimerase family.

Sequence caution

The sequence AAP98469.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysine biosynthetic process via diaminopimelate

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondiaminopimelate epimerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Diaminopimelate epimerase HAMAP-Rule MF_00197
PRO_0000149831

Regions

Region72 – 743Substrate binding By similarity
Region197 – 1982Substrate binding By similarity
Region208 – 2092Substrate binding By similarity

Sites

Active site721Proton donor/acceptor By similarity
Active site2071Proton donor/acceptor By similarity
Binding site201Substrate By similarity
Binding site451Substrate By similarity
Binding site631Substrate By similarity
Binding site1791Substrate By similarity
Site1471Important for catalytic activity By similarity
Site1971Important for catalytic activity By similarity

Amino acid modifications

Disulfide bond72 ↔ 207 HAMAP-Rule MF_00197

Sequences

Sequence LengthMass (Da)Tools
Q9Z833 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: D0679450E24FADA0

FASTA25728,323
        10         20         30         40         50         60 
MAFYSPSTIS KYFIYSGAGN RFLLGETLPE VEDVRFLCQE TRVDGFLYLK PSSCADAQLI 

        70         80         90        100        110        120 
IFNSDGSRPT MCGNGLRCAI AHLASQKGKS DISVSTDSGL YSGYFYSWDR VLVDMTLADW 

       130        140        150        160        170        180 
RASVHRLESR PDPLPKEVVC IHTGVPHAVV ILPEISTLDL SILGPFLRYH QTFSPDGVNV 

       190        200        210        220        230        240 
NFVQILGHCQ LRVRTYERGV EGETAACGTG ALASALVVSN SYGWKESIQI HTWGGELMTV 

       250 
SQNRGRVYLQ GSVTRDL 

« Hide

References

[1]"Comparative genomes of Chlamydia pneumoniae and C. trachomatis."
Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S.
Nat. Genet. 21:385-389(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CWL029.
[2]"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39."
Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. expand/collapse author list , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M.
Nucleic Acids Res. 28:1397-1406(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AR39.
[3]"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J138.
[4]"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TW-183.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001363 Genomic DNA. Translation: AAD18659.1.
AE002161 Genomic DNA. Translation: AAF73646.1.
BA000008 Genomic DNA. Translation: BAA98725.1.
AE009440 Genomic DNA. Translation: AAP98469.1. Different initiation.
PIRC86555.
F72067.
RefSeqNP_224715.1. NC_000922.1.
NP_300574.1. NC_002491.1.
NP_444785.1. NC_002179.2.
NP_876812.1. NC_005043.1.

3D structure databases

ProteinModelPortalQ9Z833.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING115713.CPn0519.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD18659; AAD18659; CPn_0519.
AAF73646; AAF73646; CP_0234.
AAP98469; AAP98469; CpB0540.
BAA98725; BAA98725; BAA98725.
GeneID1467219.
895298.
919276.
963818.
KEGGcpa:CP0234.
cpj:CPj0519.
cpn:CPn0519.
cpt:CpB0540.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0253.
HOGENOMHOG000220466.
KOK01778.
OMANGLRCVI.
OrthoDBEOG6ND0M5.
ProtClustDBPRK00450.

Enzyme and pathway databases

BioCycCPNE115711:GI7B-247-MONOMER.
CPNE115713:GHEY-535-MONOMER.
CPNE138677:GH8N-528-MONOMER.
CPNE182082:GH4N-550-MONOMER.
UniPathwayUPA00034; UER00025.

Family and domain databases

HAMAPMF_00197. DAP_epimerase.
InterProIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERPTHR31689:SF0. PTHR31689:SF0. 1 hit.
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
PROSITEPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPF_CHLPN
AccessionPrimary (citable) accession number: Q9Z833
Secondary accession number(s): Q9JQ32
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: February 19, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways