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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF), Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20SubstrateUniRule annotation1
Binding sitei63SubstrateUniRule annotation1
Active sitei72Proton donorUniRule annotation1
Sitei147Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei179SubstrateUniRule annotation1
Sitei197Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei207Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:CPn_0519, CP_0234, CpB0540
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000583 Componenti: Chromosome
  • UP000000801 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001498311 – 257Diaminopimelate epimeraseAdd BLAST257

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi182082.CpB0540

Structurei

3D structure databases

ProteinModelPortaliQ9Z833
SMRiQ9Z833
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 74Substrate bindingUniRule annotation2
Regioni197 – 198Substrate bindingUniRule annotation2
Regioni208 – 209Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E4Z Bacteria
COG0253 LUCA
HOGENOMiHOG000220466
KOiK01778
OMAiSMCGNGG
OrthoDBiPOG091H01QC

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z833-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFYSPSTIS KYFIYSGAGN RFLLGETLPE VEDVRFLCQE TRVDGFLYLK
60 70 80 90 100
PSSCADAQLI IFNSDGSRPT MCGNGLRCAI AHLASQKGKS DISVSTDSGL
110 120 130 140 150
YSGYFYSWDR VLVDMTLADW RASVHRLESR PDPLPKEVVC IHTGVPHAVV
160 170 180 190 200
ILPEISTLDL SILGPFLRYH QTFSPDGVNV NFVQILGHCQ LRVRTYERGV
210 220 230 240 250
EGETAACGTG ALASALVVSN SYGWKESIQI HTWGGELMTV SQNRGRVYLQ

GSVTRDL
Length:257
Mass (Da):28,323
Last modified:May 1, 1999 - v1
Checksum:iD0679450E24FADA0
GO

Sequence cautioni

The sequence AAP98469 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA Translation: AAD18659.1
AE002161 Genomic DNA Translation: AAF73646.1
BA000008 Genomic DNA Translation: BAA98725.1
AE009440 Genomic DNA Translation: AAP98469.1 Different initiation.
PIRiC86555
F72067
RefSeqiNP_224715.1, NC_000922.1
WP_010883157.1, NZ_LN847257.1

Genome annotation databases

EnsemblBacteriaiAAD18659; AAD18659; CPn_0519
AAF73646; AAF73646; CP_0234
AAP98469; AAP98469; CpB0540
BAA98725; BAA98725; BAA98725
GeneIDi895298
KEGGicpa:CP_0234
cpj:dapF
cpn:CPn0519
cpt:CpB0540
PATRICifig|115713.3.peg.578

Similar proteinsi

Entry informationi

Entry nameiDAPF_CHLPN
AccessioniPrimary (citable) accession number: Q9Z833
Secondary accession number(s): Q9JQ32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health