Q9Z820 (RISA_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase alpha chain
EC=2.5.1.9

Gene names

Name:	ribE
Synonyms:	ribC
Ordered Locus Names:	CPn_0532, CP_0220, CpB0553

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Oligomer that consist of 3 alpha subunits and 60 beta subunits By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Riboflavin synthase alpha chain		PRO_0000068164
Regions
	Repeat	1 – 97	97	Lumazine-binding 1
	Repeat	98 – 190	93	Lumazine-binding 2

Sequences

	Sequence		Length	Mass (Da)
	Q9Z820 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: CB92A79CA7FB9202	FASTA	200	21,701
	10 20 30 40 50 60 MFSGIIQELG EVCFFEAQGN GLSLGIKSTP LFVTPLVTGD SVAVDGVCLT LTSCNESKIF 70 80 90 100 110 120 FDVIPETLAC TTLGEKRCSD QVNLEAALKM GDSIGGHLLS GHVFGTAEIF LIKENRYYFR 130 140 150 160 170 180 GSKELSQYLF EKGFIAIDGI SLTLVSVDSD TFSVGLIPET LQRTTLGKKR EGERVNIEID 190 200 MSTKIQVDTV KRILASSGKD

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18672.1. AE002161 Genomic DNA. Translation: AAF38089.1. BA000008 Genomic DNA. Translation: BAA98738.1. AE009440 Genomic DNA. Translation: AAP98482.1.
PIR	E72066. H86556.
RefSeq	NP_224728.1. NC_000922.1. NP_300587.1. NC_002491.1. NP_444771.1. NC_002179.2. NP_876825.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z820.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1467232. 895387. 919295. 963827.
GenomeReviews	Gene locus CPn_0532 in contig AE001363_GR. Gene locus CP_0220 in contig AE002161_GR. Gene locus CpB0553 in contig AE009440_GR. Gene locus ribE in contig BA000008_GR.
KEGG	cpa:CP0220. cpn:CPn0532. cpt:CpB0553.
TIGR	CP_0220.
Phylogenomic databases
HOGENOM	HBG289809.
OMA	HILSGHV.
ProtClustDB	PRK09289.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0220-MONOMER. CPNE115713:CPN0532-MONOMER. CPNE138677:CPJ0532-MONOMER. CPNE182082:CPB0553-MONOMER.
Family and domain databases
InterPro	IPR023366. ATPase_F1/A1-cplx_a_su_N. IPR001783. Lumazine-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Gene3D	G3DSA:2.40.30.20. G3DSA:2.40.30.20. 2 hits.
KO	K00793.
PANTHER	PTHR21098. Lumazine_bd. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 2 hits.
TIGRFAMs	TIGR00187. RibE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_CHLPN

Accession

Primary (citable) accession number: Q9Z820
Secondary accession number(s): Q9JQ33

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program