Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei256 – 2561ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-201-MONOMER.
CPNE115713:GHEY-579-MONOMER.
CPNE138677:GH8N-571-MONOMER.
CPNE182082:GH4N-594-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CPn_0560, CP_0190, CpB0582
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000802 Componenti: Chromosome UP000000583 Componenti: Chromosome UP000000801 Componenti: Chromosome UP000000424 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Glutamate--tRNA ligasePRO_0000119539Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi115713.CPn0560.

Structurei

3D structure databases

ProteinModelPortaliQ9Z7Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionAdd
BLAST
Motifi253 – 2575"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiITESCRY.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z7Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNWENVRVRV APSPTGDPHV GTAYMALFNE IFAKRFKGKM ILRIEDTDRT
60 70 80 90 100
RSRQDYEENI FSALRWCGIQ WDEGPDVGGP YGPYRQSERT KIYQGYVETL
110 120 130 140 150
LKTDCAYKCF ATPQELAEMR AVASTLGYRG GYDRRYRYLS PEEVASREAA
160 170 180 190 200
GQPYTIRLKV PLSGECVFED YSKGRVVFPW ADVDDQVLVK SDGFPTYHFA
210 220 230 240 250
NVIDDHLMGI THVLRGEEWL SSTPKHLLLY EAFGWEPPVF LHMPLLLNPD
260 270 280 290 300
GTKLSKRKNP TSIFYYRDSG YVKEAFVNFL TLMGYSMEGD EEVYSLERII
310 320 330 340 350
ETFNPRRIGK SGAVFDIQKL DWMNKHYLNH EGSPECLLKE LQGWLLNDEF
360 370 380 390 400
FLKILPLCQS RITTLAEFIN LTSFFFSGLL EYRVEELLPQ ALSPEKAAIL
410 420 430 440 450
LYSYVKYLEK TDQWTKETCY LGSKWLAQAF NVHHKKAIIP LLYVAITGKK
460 470 480 490 500
QGLPLFDSIE ILGKPRARAR LVYAEKLLGG VPKKLAATVD KFMQREDFEE

ATFDL
Length:505
Mass (Da):58,206
Last modified:April 30, 1999 - v1
Checksum:i91B580D7C31E6695
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18700.1.
AE002161 Genomic DNA. Translation: AAF38063.1.
BA000008 Genomic DNA. Translation: BAA98766.1.
AE009440 Genomic DNA. Translation: AAP98511.1.
PIRiC72064.
D86560.
RefSeqiNP_224756.1. NC_000922.1.
NP_300615.1. NC_002491.1.
NP_444741.1. NC_002179.2.
NP_876854.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD18700; AAD18700; CPn_0560.
AAF38063; AAF38063; CP_0190.
AAP98511; AAP98511; CpB0582.
BAA98766; BAA98766; BAA98766.
GeneIDi895358.
962974.
KEGGicpa:CP0190.
cpj:CPj0560.
cpn:CPn0560.
cpt:CpB0582.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18700.1.
AE002161 Genomic DNA. Translation: AAF38063.1.
BA000008 Genomic DNA. Translation: BAA98766.1.
AE009440 Genomic DNA. Translation: AAP98511.1.
PIRiC72064.
D86560.
RefSeqiNP_224756.1. NC_000922.1.
NP_300615.1. NC_002491.1.
NP_444741.1. NC_002179.2.
NP_876854.1. NC_005043.1.

3D structure databases

ProteinModelPortaliQ9Z7Z3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi115713.CPn0560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD18700; AAD18700; CPn_0560.
AAF38063; AAF38063; CP_0190.
AAP98511; AAP98511; CpB0582.
BAA98766; BAA98766; BAA98766.
GeneIDi895358.
962974.
KEGGicpa:CP0190.
cpj:CPj0560.
cpn:CPn0560.
cpt:CpB0582.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiITESCRY.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-201-MONOMER.
CPNE115713:GHEY-579-MONOMER.
CPNE138677:GH8N-571-MONOMER.
CPNE182082:GH4N-594-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiSYE_CHLPN
AccessioniPrimary (citable) accession number: Q9Z7Z3
Secondary accession number(s): Q9JQI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2000
Last sequence update: April 30, 1999
Last modified: March 31, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.