Q9Z7T3 (RUVC_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease RuvC
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC

Gene names

Name:	ruvC
Ordered Locus Names:	CPn_0621, CP_0126, CpB0647

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034
Sequence similarities	Belongs to the RuvC family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 168	168	Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034		PRO_0000183088
Sites
	Metal binding	9	1	Magnesium By similarity
	Metal binding	70	1	Magnesium By similarity
	Metal binding	145	1	Magnesium By similarity
	Metal binding	148	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z7T3 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3845FAC6DC0AFD1B	FASTA	168	18,404
	10 20 30 40 50 60 MSELIIGVDP GTIVAGYAII AVEQRYQLRP YSYGAIRLSS DMPLPMRYKT LFEQLSGVLD 70 80 90 100 110 120 DTQPNAMVLE TQFVNKNPQS TMKLAMARGI VLLAAAQRDI LIFEYAPNVA KKAVVGKGHA 130 140 150 160 SKRQVQVMVS KILNVPEVLH PSNEDIADAF ALAICHTHVA RSPLCGVR

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18760.1. AE002161 Genomic DNA. Translation: AAF38009.1. BA000008 Genomic DNA. Translation: BAA98828.1. AE009440 Genomic DNA. Translation: AAP98576.1.
PIR	A72057. B86568.
RefSeq	NP_224817.1. NC_000922.1. NP_300677.1. NC_002491.1. NP_444678.1. NC_002179.2. NP_876919.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z7T3.
ModBase	Search...
Protein-protein interaction databases
STRING	115713.CPn0621.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD18760; AAD18760; CPn_0621. AAF38009; AAF38009; CP_0126. AAP98576; AAP98576; CpB0647. BAA98828; BAA98828; BAA98828.
GeneID	1467326. 895409. 919385. 963017.
KEGG	cpa:CP0126. cpj:CPj0621. cpn:CPn0621. cpt:CpB0647.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0817.
HOGENOM	HOG000012181.
KO	K01159.
OMA	ILHMIKL.
ProtClustDB	PRK00039.
Enzyme and pathway databases
BioCyc	CPNE115711:GI7B-133-MONOMER. CPNE115713:GHEY-646-MONOMER. CPNE138677:GH8N-637-MONOMER. CPNE182082:GH4N-663-MONOMER.
Family and domain databases
HAMAP	MF_00034. RuvC.
InterPro	IPR012337. RNaseH-like_dom. IPR020563. X-over_junc_endoDNase_Mg_BS. IPR002176. X-over_junc_endoDNase_RuvC. [Graphical view]
Pfam	PF02075. RuvC. 1 hit. [Graphical view]
PRINTS	PR00696. RSOLVASERUVC.
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00228. ruvC. 1 hit.
PROSITE	PS01321. RUVC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUVC_CHLPN

Accession

Primary (citable) accession number: Q9Z7T3
Secondary accession number(s): Q9JQH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 29, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program