Q9Z7I4 (KDSA_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	CPn_0721, CP_0025, CpB0749

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.
Sequence caution	The sequence AAF37920.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000187117
Experimental info
	Sequence conflict	125	1	N → H in BAA98928. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9Z7I4 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: E184BF3D8BB36C46	FASTA	269	28,994
	10 20 30 40 50 60 MFNNKMILIA GPCVIEGEDI TLEIAGKLQS ILAPYSDRIQ WFFKSSYDKA NRSSLNSFRG 70 80 90 100 110 120 PGLTEGLRIL AKVKETFGVG ILTDVHTPQD AYAAAEVCNI LQVPAFLCRQ TDLLVATAET 130 140 150 160 170 180 GAIVNLKKGQ FLSPWDMEGP INKVLSTGNN KILLTERGCS FGYNNLVSDM RSIPVLSRSG 190 200 210 220 230 240 FPVIFDATHS VQLPGALSTE SGGLTEFVPT LSRAALAAGA HGLFIETHTN PKIAKSDAAS 250 260 MLSLEEFAAL LPTWDQLFTC VSSFDMVSA

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18860.1. AE002161 Genomic DNA. Translation: AAF37920.1. Different initiation. BA000008 Genomic DNA. Translation: BAA98928.1. AE009440 Genomic DNA. Translation: AAP98678.1.
PIR	A72044. E81621. F86580.
RefSeq	NP_224917.1. NC_000922.1. NP_300777.1. NC_002491.1. NP_444577.1. NC_002179.2. NP_877021.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z7I4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1467428. 895556. 919487. 963646.
GenomeReviews	Gene locus CPn_0721 in contig AE001363_GR. Gene locus CP_0025 in contig AE002161_GR. Gene locus CpB0749 in contig AE009440_GR. Gene locus kdsA in contig BA000008_GR.
KEGG	cpa:CP0025. cpn:CPn0721. cpt:CpB0749.
TIGR	CP_0025.
Phylogenomic databases
HOGENOM	HBG575990.
OMA	ESRDHAF.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0025-MONOMER. CPNE115713:CPN0721-MONOMER. CPNE138677:CPJ0721-MONOMER. CPNE182082:CPB0749-MONOMER.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_CHLPN

Accession

Primary (citable) accession number: Q9Z7I4
Secondary accession number(s): Q9JSB6, Q9K2E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program