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Protein

Folate synthesis bifunctional protein

Gene

folKP

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate.
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate.By similarity

Pathway:itetrahydrofolate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Probable dihydroneopterin aldolase (folB)
  4. Folate synthesis bifunctional protein (folKP)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Pathway:itetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Folate synthesis bifunctional protein (folKP)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi188 – 1881MagnesiumBy similarity
Binding sitei196 – 1961SubstrateBy similarity
Binding sitei268 – 2681SubstrateBy similarity
Binding sitei288 – 2881SubstrateBy similarity
Binding sitei358 – 3581SubstrateBy similarity
Binding sitei394 – 3941SubstrateBy similarity
Binding sitei428 – 4281SubstrateBy similarity
Binding sitei430 – 4301SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Folate biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-1151-MONOMER.
CPNE115713:GHEY-792-MONOMER.
CPNE138677:GH8N-784-MONOMER.
CPNE182082:GH4N-811-MONOMER.
UniPathwayiUPA00077; UER00155.
UPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Folate synthesis bifunctional protein
Including the following 2 domains:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.3)
Alternative name(s):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Short name:
PPPK
7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Short name:
HPPK
Dihydropteroate synthase (EC:2.5.1.15)
Short name:
DHPS
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene namesi
Name:folKP
Synonyms:folP
Ordered Locus Names:CPn_0758, CP_1114, CpB0786
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000583 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450Folate synthesis bifunctional proteinPRO_0000168240Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi115713.CPn0758.

Structurei

3D structure databases

ProteinModelPortaliQ9Z7E8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini181 – 440260Pterin-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 166166HPPKAdd
BLAST
Regioni228 – 2292Substrate bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the HPPK family.Curated
Contains 1 pterin-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0294.
HOGENOMiHOG000063856.
KOiK13941.
OMAiKTHVMAI.
OrthoDBiEOG67T5P5.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
3.30.70.560. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000550. Hppk.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF01288. HPPK. 1 hit.
PF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
SSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z7E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPRFVCLS LGSNLGNRFK NLQIARTLLG EQAVLGLRSS VILETEALLL
60 70 80 90 100
PGSPPEWDLP YFNSVLVGET TLSLRELLVT IKQIEKVVGR AEESPPWSPR
110 120 130 140 150
TIDVDILLYG DESFCCDHTE ITIPLSNLLS RPFLIALIAS LCPYRRFCTQ
160 170 180 190 200
GSPYHNFTFG ELAHHLPSPP GMIRRSLSPD TMLMGVVNVT NDSMSDGGMF
210 220 230 240 250
LDPEKAVAQA EKLFTEGAAV IDFGAQATNP KVKQFLSVDQ EWERLEPVLR
260 270 280 290 300
LLKETWSNRK QYPIISLDTF YPEIILRAMD IYPIQWINDV SGGSQSMAEV
310 320 330 340 350
ARDCELSLVM NHSSSLPVDP KNILSFSVPI GEQLLSWGEK QLKMFSDVGL
360 370 380 390 400
NANQVIFDPG IGFGKGAAQS LATLYEIAKF KRLGCPILIG HSRKSFLSLF
410 420 430 440 450
GNHDPKDRDW ETVGLSILLQ QQGVDYLRVH NVAAHQKALS VAACEACAPI
Length:450
Mass (Da):49,815
Last modified:May 1, 1999 - v1
Checksum:iD644AB80EF4C9A8C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18896.1.
AE002161 Genomic DNA. Translation: AAF38881.1.
BA000008 Genomic DNA. Translation: BAA98966.1.
AE009440 Genomic DNA. Translation: AAP98715.1.
PIRiD86585.
G72039.
RefSeqiNP_224953.1. NC_000922.1.

Genome annotation databases

EnsemblBacteriaiAAD18896; AAD18896; CPn_0758.
AAF38881; AAF38881; CP_1114.
AAP98715; AAP98715; CpB0786.
BAA98966; BAA98966; BAA98966.
GeneIDi894959.
KEGGicpa:CP1114.
cpj:CPj0758.
cpn:CPn0758.
cpt:CpB0786.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18896.1.
AE002161 Genomic DNA. Translation: AAF38881.1.
BA000008 Genomic DNA. Translation: BAA98966.1.
AE009440 Genomic DNA. Translation: AAP98715.1.
PIRiD86585.
G72039.
RefSeqiNP_224953.1. NC_000922.1.

3D structure databases

ProteinModelPortaliQ9Z7E8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi115713.CPn0758.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD18896; AAD18896; CPn_0758.
AAF38881; AAF38881; CP_1114.
AAP98715; AAP98715; CpB0786.
BAA98966; BAA98966; BAA98966.
GeneIDi894959.
KEGGicpa:CP1114.
cpj:CPj0758.
cpn:CPn0758.
cpt:CpB0786.

Phylogenomic databases

eggNOGiCOG0294.
HOGENOMiHOG000063856.
KOiK13941.
OMAiKTHVMAI.
OrthoDBiEOG67T5P5.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00155.
UPA00077; UER00156.
BioCyciCPNE115711:GI7B-1151-MONOMER.
CPNE115713:GHEY-792-MONOMER.
CPNE138677:GH8N-784-MONOMER.
CPNE182082:GH4N-811-MONOMER.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
3.30.70.560. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000550. Hppk.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF01288. HPPK. 1 hit.
PF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
SSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiFOLKP_CHLPN
AccessioniPrimary (citable) accession number: Q9Z7E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.