Q9Z7A4 (SYW_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	CPn_0802, CP_1069, CpB0831

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140_B
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Tryptophan--tRNA ligase HAMAP-Rule MF_00140_B		PRO_0000136619
Regions
	Motif	13 – 21	9	"HIGH" region HAMAP-Rule MF_00140_B
	Motif	202 – 206	5	"KMSKS" region HAMAP-Rule MF_00140_B
Sites
	Binding site	205	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z7A4 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6204B07F34385169	FASTA	344	39,283
	10 20 30 40 50 60 MNKKKRVLTG DRPTGKLHLG HWVGSIKNRL ELQNSPEYDC FFIIADLHTL TTKIRKEEVL 70 80 90 100 110 120 DVDNHIYEVL ADWLSVGIDP TKSIIYLQSA IPEIYELHLL FSMLISINRV MGIPSLKDMA 130 140 150 160 170 180 RNASIEEGSL SYGLIGYPIL QSADILLAKA QFVPVGKDNE AHVELTRDIA RNFNRLYGQV 190 200 210 220 230 240 FPEPEVLQGE LTSLVGIDGQ GKMSKSANNA IYLSDSDATI TEKVRKMYTD PNRIRATTPG 250 260 270 280 290 300 RVEGNPLFIY HDIFNPHKDE VEEFKARYRQ GCIKDIEVKA RLAEELIHFL KPIKERRSEF 310 320 330 340 LSKPLALQNV LEDGTHKMRE VAKVTMEEVH DKFGFSHKWR SLLK

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18940.1. AE002161 Genomic DNA. Translation: AAF38841.1. BA000008 Genomic DNA. Translation: BAA99010.1. AE009440 Genomic DNA. Translation: AAP98760.1.
PIR	C72034. H86590.
RefSeq	NP_224997.1. NC_000922.1. NP_300859.1. NC_002491.1. NP_445606.1. NC_002179.2. NP_877103.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z7A4.
ModBase	Search...
Protein-protein interaction databases
STRING	115713.CPn0802.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD18940; AAD18940; CPn_0802. AAF38841; AAF38841; CP_1069. AAP98760; AAP98760; CpB0831. BAA99010; BAA99010; BAA99010.
GeneID	1467510. 895541. 919559. 963553.
KEGG	cpa:CP1069. cpj:CPj0802. cpn:CPn0802. cpt:CpB0831.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000059941.
KO	K01867.
OMA	RDNESHI.
ProtClustDB	PRK00927.
Enzyme and pathway databases
BioCyc	CPNE115711:GI7B-1067-MONOMER. CPNE115713:GHEY-805-MONOMER. CPNE138677:GH8N-792-MONOMER. CPNE182082:GH4N-828-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_B. Trp_tRNA_synth_B.
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_CHLPN

Accession

Primary (citable) accession number: Q9Z7A4
Secondary accession number(s): Q9JQ80

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program