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Protein

Lipoyl synthase

Gene

lipA

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi78 – 781Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi82 – 821Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi85 – 851Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-1074-MONOMER.
CPNE115713:GHEY-867-MONOMER.
CPNE138677:GH8N-859-MONOMER.
CPNE182082:GH4N-887-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:CPn_0832, CP_1038, CpB0861
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000802 Componenti: Chromosome UP000000583 Componenti: Chromosome UP000000801 Componenti: Chromosome UP000000424 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Lipoyl synthasePRO_0000102304Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi115713.CPn0832.

Structurei

3D structure databases

ProteinModelPortaliQ9Z774.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPRLYHIR.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCRPTLNTD QPRVRKKLPE RFPKWLQRPL PQGSAFHATD ATIKRSGMPT
60 70 80 90 100
VCEEALCPNR AECWSRKTAT YLALGDVCTR SCGFCNIGHS KTPPALDPTE
110 120 130 140 150
PERIALSAKE LGLKHVVITM VARDDLEDGG AQGLVDIIQK LREELPQATT
160 170 180 190 200
EVLASDFQGN VSALHTLLDS GITIYNHNVE TVARLSPLVR HKATYARSMF
210 220 230 240 250
MLEQAANYLP DLKIKSGIMV GLGEMEGEVK QTLQDLASIG VRIVTIGQYL
260 270 280 290 300
RPSRKHLQVK SYVTPETFDY YRRVGEAMGL FVYAGPFVRS SFNADMILAS

VQDKASA
Length:307
Mass (Da):33,993
Last modified:May 1, 1999 - v1
Checksum:iF893D50F7BC90062
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18969.1.
AE002161 Genomic DNA. Translation: AAF38813.1.
BA000008 Genomic DNA. Translation: BAA99040.1.
AB035942 Genomic DNA. Translation: BAA88650.1.
AE009440 Genomic DNA. Translation: AAP98790.1.
PIRiB72031.
F86594.
RefSeqiNP_225027.1. NC_000922.1.
NP_300889.1. NC_002491.1.
NP_445575.1. NC_002179.2.
NP_877133.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD18969; AAD18969; CPn_0832.
AAF38813; AAF38813; CP_1038.
AAP98790; AAP98790; CpB0861.
BAA99040; BAA99040; BAA99040.
GeneIDi894731.
963516.
KEGGicpa:CP1038.
cpj:CPj0832.
cpn:CPn0832.
cpt:CpB0861.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD18969.1.
AE002161 Genomic DNA. Translation: AAF38813.1.
BA000008 Genomic DNA. Translation: BAA99040.1.
AB035942 Genomic DNA. Translation: BAA88650.1.
AE009440 Genomic DNA. Translation: AAP98790.1.
PIRiB72031.
F86594.
RefSeqiNP_225027.1. NC_000922.1.
NP_300889.1. NC_002491.1.
NP_445575.1. NC_002179.2.
NP_877133.1. NC_005043.1.

3D structure databases

ProteinModelPortaliQ9Z774.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi115713.CPn0832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD18969; AAD18969; CPn_0832.
AAF38813; AAF38813; CP_1038.
AAP98790; AAP98790; CpB0861.
BAA99040; BAA99040; BAA99040.
GeneIDi894731.
963516.
KEGGicpa:CP1038.
cpj:CPj0832.
cpn:CPn0832.
cpt:CpB0861.

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPRLYHIR.
OrthoDBiEOG6038ZS.

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.
BioCyciCPNE115711:GI7B-1074-MONOMER.
CPNE115713:GHEY-867-MONOMER.
CPNE138677:GH8N-859-MONOMER.
CPNE182082:GH4N-887-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "Genomic sequence comparison of two unrelated isolates of Chlamydia pneumoniae from Japan and U.S."
    Hirakawa H., Shirai M., Kuhara S.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: J138.
  5. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiLIPA_CHLPN
AccessioniPrimary (citable) accession number: Q9Z774
Secondary accession number(s): Q9JQ18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: April 1, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.