Q9Z774 (LIPA_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase
EC=2.8.1.8

Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA

Gene names

Name:	lipA
Ordered Locus Names:	CPn_0832, CP_1038, CpB0861

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Lipoyl synthase HAMAP-Rule MF_00206		PRO_0000102304
Sites
	Metal binding	52	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	57	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	63	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	78	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	82	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	85	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z774 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: F893D50F7BC90062	FASTA	307	33,993
	10 20 30 40 50 60 MKCRPTLNTD QPRVRKKLPE RFPKWLQRPL PQGSAFHATD ATIKRSGMPT VCEEALCPNR 70 80 90 100 110 120 AECWSRKTAT YLALGDVCTR SCGFCNIGHS KTPPALDPTE PERIALSAKE LGLKHVVITM 130 140 150 160 170 180 VARDDLEDGG AQGLVDIIQK LREELPQATT EVLASDFQGN VSALHTLLDS GITIYNHNVE 190 200 210 220 230 240 TVARLSPLVR HKATYARSMF MLEQAANYLP DLKIKSGIMV GLGEMEGEVK QTLQDLASIG 250 260 270 280 290 300 VRIVTIGQYL RPSRKHLQVK SYVTPETFDY YRRVGEAMGL FVYAGPFVRS SFNADMILAS VQDKASA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"Genomic sequence comparison of two unrelated isolates of Chlamydia pneumoniae from Japan and U.S." Hirakawa H., Shirai M., Kuhara S. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: J138.
[5]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD18969.1. AE002161 Genomic DNA. Translation: AAF38813.1. BA000008 Genomic DNA. Translation: BAA99040.1. AB035942 Genomic DNA. Translation: BAA88650.1. AE009440 Genomic DNA. Translation: AAP98790.1.
PIR	B72031. F86594.
RefSeq	NP_225027.1. NC_000922.1. NP_300889.1. NC_002491.1. NP_445575.1. NC_002179.2. NP_877133.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z774.
ModBase	Search...
Protein-protein interaction databases
STRING	115713.CPn0832.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD18969; AAD18969; CPn_0832. AAF38813; AAF38813; CP_1038. AAP98790; AAP98790; CpB0861. BAA99040; BAA99040; BAA99040.
GeneID	1467540. 894731. 919598. 963516.
KEGG	cpa:CP1038. cpj:CPj0832. cpn:CPn0832. cpt:CpB0861.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0320.
HOGENOM	HOG000235997.
KO	K03644.
OMA	DPHEPQR.
ProtClustDB	PRK05481.
Enzyme and pathway databases
BioCyc	CPNE115711:GI7B-1036-MONOMER. CPNE115713:GHEY-835-MONOMER. CPNE138677:GH8N-822-MONOMER. CPNE182082:GH4N-858-MONOMER.
UniPathway	UPA00538; UER00593.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIPA_CHLPN

Accession

Primary (citable) accession number: Q9Z774
Secondary accession number(s): Q9JQ18

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program