Riboflavin biosynthesis protein ribBA

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Q9Z734 (RIBBA_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Riboflavin biosynthesis protein ribBA

Including the following 2 domains:

3,4-dihydroxy-2-butanone 4-phosphate synthase
Short name=DHBP synthase
EC=4.1.99.12
GTP cyclohydrolase-2
EC=3.5.4.25
Alternative name(s):
GTP cyclohydrolase II

Gene names

Name:	ribBA
Synonyms:	ribA/ribB
Ordered Locus Names:	CPn_0872, CP_0997, CpB0901

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_01283 Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity. HAMAP MF_01283
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_01283 GTP + 3 H₂O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate. HAMAP MF_01283
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. Binds 1 zinc ion per subunit By similarity. HAMAP MF_01283
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_01283 Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP MF_01283
Sequence similarities	In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	GTP-binding Magnesium Manganese Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: EC GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase II activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 418

418

Riboflavin biosynthesis protein ribBA HAMAP MF_01283

PRO_0000151724

Regions

Nucleotide binding

262 – 266

GTP By similarity

Nucleotide binding

306 – 308

GTP By similarity

Region

1 – 211

211

DHBP synthase HAMAP MF_01283

Region

37 – 38

D-ribulose 5-phosphate binding By similarity

Region

150 – 154

D-ribulose 5-phosphate binding By similarity

Region

212 – 418

207

GTP cyclohydrolase II HAMAP MF_01283

Sites

Active site

340

Proton acceptor; for GTP cyclohydrolase activity Potential

Active site

342

Nucleophile; for GTP cyclohydrolase activity By similarity

Metal binding

Magnesium or manganese 1 By similarity

Metal binding

Magnesium or manganese 2 By similarity

Metal binding

153

Magnesium or manganese 2 By similarity

Metal binding

267

Zinc; catalytic By similarity

Metal binding

278

Zinc; catalytic By similarity

Metal binding

280

Zinc; catalytic By similarity

Binding site

D-ribulose 5-phosphate By similarity

Binding site

174

D-ribulose 5-phosphate By similarity

Binding site

283

GTP By similarity

Binding site

328

GTP By similarity

Binding site

363

GTP By similarity

Binding site

368

GTP By similarity

Site

136

Essential for DHBP synthase activity By similarity

Site

174

Essential for DHBP synthase activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z734 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 7A5A214BB0EC0E32
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FASTA

418

45,846

        10         20         30         40         50         60 
MIETREEVGS ANFVSLERAI EDLRAGKFVI VVDEASREDE GDLIIAGEKI TVEKMTFLLQ 

        70         80         90        100        110        120 
HTTGVVCAAL SQERLLSLDL PPMVKDNRCR FKTPFTVSVD AAHGVTTGVS AADRTKVVQL 

       130        140        150        160        170        180 
LADPKSKPED FISPGHFFPL ASSPGGVLKR AGHTESTVDL MELAGLQPCG VLAELVNEDY 

       190        200        210        220        230        240 
SMMRLPQILE FARKHNIAVI PVTSIIAHRM LSDRLVSKIS SARLPTIYGD FTIHVYESLL 

       250        260        270        280        290        300 
EGMQHLALVK GNVAGKSNVL VRVHSECVTG DILGSKRCDC GEQLSSAMSY IAEKGTGVLV 

       310        320        330        340        350        360 
YLRGQEGRGI GLGHKVRAYA LQDNGYDTVD ANLAMGFPVD SREYGIGAQI LVDLKLTTIK 

       370        380        390        400        410 
LITHNPQKYF GLQGFGLSIT ERVPLPVRIS EDNEQYLRTK QERMGHWLDL PCCNNRVQ

« Hide

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD19010.1. AE002161 Genomic DNA. Translation: AAF38775.1. BA000008 Genomic DNA. Translation: BAA99080.1. AE009440 Genomic DNA. Translation: AAP98830.1.
PIR	F86599. H72026.
RefSeq	NP_225067.1. NC_000922.1. NP_300929.1. NC_002491.1. NP_445534.1. NC_002179.2. NP_877173.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z734.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1467580. 895694. 919635. 963470.
GenomeReviews	Gene locus CPn_0872 in contig AE001363_GR. Gene locus CP_0997 in contig AE002161_GR. Gene locus CpB0901 in contig AE009440_GR. Gene locus ribBA in contig BA000008_GR.
KEGG	cpa:CP0997. cpn:CPn0872. cpt:CpB0901.
TIGR	CP_0997.
Phylogenomic databases
HOGENOM	HBG735778.
OMA	RCDCRMQ.
ProtClustDB	PRK09311.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0997-MONOMER. CPNE115713:CPN0872-MONOMER. CPNE138677:CPJ0872-MONOMER. CPNE182082:CPB0901-MONOMER.
Family and domain databases
HAMAP	MF_01283. RibBA. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. IPR000926. GTP_CycHdrlaseII_RibA. IPR016299. Riboflavin_synth_RibBA. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
KO	K14652.
Pfam	PF00926. DHBP_synthase. 1 hit. PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
PIRSF	PIRSF001259. RibA. 1 hit.
SUPFAM	SSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMs	TIGR00505. RibA. 1 hit. TIGR00506. RibB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBBA_CHLPN

Accession

Primary (citable) accession number: Q9Z734
Secondary accession number(s): Q9JQ68

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents