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Protein

Na(+)-translocating NADH-quinone reductase subunit F

Gene

nqrF

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway.UniRule annotation

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • FADUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi76 – 761Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi85 – 851Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi117 – 1171Iron-sulfur (2Fe-2S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD, Sodium, Ubiquinone

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-1014-MONOMER.
CPNE115713:GHEY-923-MONOMER.
CPNE138677:GH8N-915-MONOMER.
CPNE182082:GH4N-943-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit FUniRule annotation (EC:1.6.5.8UniRule annotation)
Short name:
Na(+)-NQR subunit FUniRule annotation
Short name:
Na(+)-translocating NQR subunit FUniRule annotation
Alternative name(s):
NQR complex subunit FUniRule annotation
NQR-1 subunit FUniRule annotation
Gene namesi
Name:nqrFUniRule annotation
Synonyms:nqr6
Ordered Locus Names:CPn_0883, CP_0983, CpB0912
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000583 Componenti: Chromosome
  • UP000000801 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431Na(+)-translocating NADH-quinone reductase subunit FPRO_0000074491Add
BLAST

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation

Protein-protein interaction databases

STRINGi115713.CPn0883.

Structurei

3D structure databases

ProteinModelPortaliQ9Z723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 133952Fe-2S ferredoxin-typeUniRule annotationAdd
BLAST
Domaini136 – 286151FAD-binding FR-typeUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni289 – 413125CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the NqrF family.UniRule annotation
Contains 1 2Fe-2S ferredoxin-type domain.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107RKJ. Bacteria.
COG2871. LUCA.
HOGENOMiHOG000263661.
KOiK00351.
OMAiEEHGIIM.
OrthoDBiPOG091H05VQ.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 2 hits.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z723-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTWLSGLYFI CIASLIFCAI GVILAGVILL SRKLFIKVHP CKLKINDNEE
60 70 80 90 100
LTKTVESGQT LLVSLLSSGI PIPSPCGGKA TCKQCKVRVV KNADEPLETD
110 120 130 140 150
RSTFSKRQLE EGWRLSCQCK VQHDMSLEIE ERYLNASSWE GTVISNDNVA
160 170 180 190 200
TFIKELVVAV DPNKPIPFKP GGYLQITVPS YKTNSSDWKQ TMAPEYYSDW
210 220 230 240 250
EHFHLFDQVI DNSQLPADSA NKAYSLASYP AELPTIKFNI RIATPPFING
260 270 280 290 300
KPNSEIPWGV CSSYVFSLKP GDKITVSGPY GESFMKDDDR PLIFLIGGAG
310 320 330 340 350
SSFGRSHILD LLLNKHSKRE IDLWYGARSL KENIYQEEYE NLERQFPNFH
360 370 380 390 400
YHLVLSEPLP EDIAAGWDKD DPTKTNFLFR AFNLGQLSRL DNPEDYLYYV
410 420 430
CGPPLHNSSI LKLLGDYGVE RSSIILDDFG S
Length:431
Mass (Da):48,500
Last modified:May 1, 1999 - v1
Checksum:iD6D9A7A474C62708
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41L → P in BAA99091 (PubMed:10871362).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19021.1.
AE002161 Genomic DNA. Translation: AAF38762.1.
BA000008 Genomic DNA. Translation: BAA99091.1.
AE009440 Genomic DNA. Translation: AAP98841.1.
PIRiA86601.
G72022.
RefSeqiNP_225078.1. NC_000922.1.
WP_010883518.1. NZ_LN847257.1.

Genome annotation databases

EnsemblBacteriaiAAD19021; AAD19021; CPn_0883.
AAF38762; AAF38762; CP_0983.
AAP98841; AAP98841; CpB0912.
BAA99091; BAA99091; BAA99091.
GeneIDi895388.
KEGGicpa:CP_0983.
cpj:nqr6.
cpn:CPn0883.
cpt:CpB0912.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19021.1.
AE002161 Genomic DNA. Translation: AAF38762.1.
BA000008 Genomic DNA. Translation: BAA99091.1.
AE009440 Genomic DNA. Translation: AAP98841.1.
PIRiA86601.
G72022.
RefSeqiNP_225078.1. NC_000922.1.
WP_010883518.1. NZ_LN847257.1.

3D structure databases

ProteinModelPortaliQ9Z723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi115713.CPn0883.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD19021; AAD19021; CPn_0883.
AAF38762; AAF38762; CP_0983.
AAP98841; AAP98841; CpB0912.
BAA99091; BAA99091; BAA99091.
GeneIDi895388.
KEGGicpa:CP_0983.
cpj:nqr6.
cpn:CPn0883.
cpt:CpB0912.

Phylogenomic databases

eggNOGiENOG4107RKJ. Bacteria.
COG2871. LUCA.
HOGENOMiHOG000263661.
KOiK00351.
OMAiEEHGIIM.
OrthoDBiPOG091H05VQ.

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-1014-MONOMER.
CPNE115713:GHEY-923-MONOMER.
CPNE138677:GH8N-915-MONOMER.
CPNE182082:GH4N-943-MONOMER.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 2 hits.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQRF_CHLPN
AccessioniPrimary (citable) accession number: Q9Z723
Secondary accession number(s): Q9JS95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.