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Q9Z714

- SYA_CHLPN

UniProt

Q9Z714 - SYA_CHLPN

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Protein
Alanine--tRNA ligase
Gene
alaS, CPn_0892, CP_0974, CpB0923
Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi558 – 5581Zinc Reviewed prediction
Metal bindingi562 – 5621Zinc Reviewed prediction
Metal bindingi660 – 6601Zinc Reviewed prediction
Metal bindingi664 – 6641Zinc Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-1005-MONOMER.
CPNE115713:GHEY-932-MONOMER.
CPNE138677:GH8N-924-MONOMER.
CPNE182082:GH4N-954-MONOMER.
BRENDAi2.5.1.55. 1311.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligase (EC:6.1.1.7)
Alternative name(s):
Alanyl-tRNA synthetase
Short name:
AlaRS
Gene namesi
Name:alaS
Ordered Locus Names:CPn_0892, CP_0974, CpB0923
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000424: Chromosome, UP000000583: Chromosome, UP000000801: Chromosome, UP000000802: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 872872Alanine--tRNA ligaseUniRule annotation
PRO_0000075087Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi115713.CPn0892.

Structurei

3D structure databases

ProteinModelPortaliQ9Z714.

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156965.
KOiK01872.
OMAiWASKGSP.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z714-1 [UniParc]FASTAAdd to Basket

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MLSNTIRSNF LKFYANRHHT ILPSSPVFPH NDPSILFTNA GMNQFKDIFL    50
NKEKVSYSRA TTSQKCIRAG GKHNDLDNVG HTSRHLTFFE MLGNFSFGDY 100
FKAEAIAFAW EVSLSVFNFN PEGIYATVHE KDDEAFALWE AYLPTDRIFR 150
LTDKDNFWSM ANTGPCGYCS ELLFDRGPSF GNASSPLDDT DGERFLEYWN 200
LVFMEFNRTS EGSLLALPNK HVDTGAGLER LVSLIAGTHT VFEADVLREL 250
IAKTEQLSGK VYHPDDSGAA FRVIADHVRS LSFAIADGLL PGNTERGYVL 300
RKILRRSVNY GRRLGFRNPF LAEIVPSLAD AMGEAYPELK NSLSQIQKVL 350
TLEEESFFKT LDRGGNLLQQ VLKSSSSSSC ISGEDAFKLK DTYGMPIDEI 400
SLLAKDYDYS VDMDTFHKLE QEAKERSRKN VVQSQGTSES IYNELHLTSE 450
FIGYDHLSCD TFIEAIISKD HIVSSLQEKQ EGAIVLKVSP FYAEKGGQVG 500
DSGEIFCSEG TFIVTHTTSP KAGLIVHHGR ISQGSLTVEA AVTAQVNRYR 550
RKRIANNHTA CHLLHKALEI TLGDHIRQAG SYVDDTKIRL DFTHPQAISP 600
EDLLCIETLV NESIRENEPV DIREALYSDV MNSSEIKQFF GDKYSDVVRV 650
VSAGHSHELC GGTHAEATGD IGFFRITKEH AVAMGIRRIE AVTGEKAEAT 700
VHQQSEVLEE IATLLQVPRD QIVSRLTATL DERKQQDKRL NELENSLIQT 750
KLDKLIHNCH QRQGITCLVH HLAEHENHRL QQYAQCLHQR IPEKLISLWT 800
TEKNGKYIVL SRVSDDLITQ GVHAQDLLKA VLTPCGGRWG GKDQSAQGSA 850
PALPATEVLN ETLWQWISTQ LI 872
Length:872
Mass (Da):97,670
Last modified:May 1, 1999 - v1
Checksum:iDAD99C43E273CAF8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001363 Genomic DNA. Translation: AAD19030.1.
AE002161 Genomic DNA. Translation: AAF38754.1.
BA000008 Genomic DNA. Translation: BAA99100.1.
AE009440 Genomic DNA. Translation: AAP98852.1.
PIRiB86602.
H72023.
RefSeqiNP_225087.1. NC_000922.1.
NP_300949.1. NC_002491.1.
NP_445511.1. NC_002179.2.
NP_877195.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD19030; AAD19030; CPn_0892.
AAF38754; AAF38754; CP_0974.
AAP98852; AAP98852; CpB0923.
BAA99100; BAA99100; BAA99100.
GeneIDi1467602.
895680.
919656.
963442.
KEGGicpa:CP0974.
cpj:CPj0892.
cpn:CPn0892.
cpt:CpB0923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001363 Genomic DNA. Translation: AAD19030.1 .
AE002161 Genomic DNA. Translation: AAF38754.1 .
BA000008 Genomic DNA. Translation: BAA99100.1 .
AE009440 Genomic DNA. Translation: AAP98852.1 .
PIRi B86602.
H72023.
RefSeqi NP_225087.1. NC_000922.1.
NP_300949.1. NC_002491.1.
NP_445511.1. NC_002179.2.
NP_877195.1. NC_005043.1.

3D structure databases

ProteinModelPortali Q9Z714.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 115713.CPn0892.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD19030 ; AAD19030 ; CPn_0892 .
AAF38754 ; AAF38754 ; CP_0974 .
AAP98852 ; AAP98852 ; CpB0923 .
BAA99100 ; BAA99100 ; BAA99100 .
GeneIDi 1467602.
895680.
919656.
963442.
KEGGi cpa:CP0974.
cpj:CPj0892.
cpn:CPn0892.
cpt:CpB0923.

Phylogenomic databases

eggNOGi COG0013.
HOGENOMi HOG000156965.
KOi K01872.
OMAi WASKGSP.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci CPNE115711:GI7B-1005-MONOMER.
CPNE115713:GHEY-932-MONOMER.
CPNE138677:GH8N-924-MONOMER.
CPNE182082:GH4N-954-MONOMER.
BRENDAi 2.5.1.55. 1311.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiSYA_CHLPN
AccessioniPrimary (citable) accession number: Q9Z714
Secondary accession number(s): Q9JQE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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