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Q9Z714

- SYA_CHLPN

UniProt

Q9Z714 - SYA_CHLPN

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Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi558 – 5581ZincUniRule annotation
Metal bindingi562 – 5621ZincUniRule annotation
Metal bindingi660 – 6601ZincUniRule annotation
Metal bindingi664 – 6641ZincUniRule annotation

GO - Molecular functioni

  1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-1005-MONOMER.
CPNE115713:GHEY-932-MONOMER.
CPNE138677:GH8N-924-MONOMER.
CPNE182082:GH4N-954-MONOMER.
BRENDAi2.5.1.55. 1311.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
Alternative name(s):
Alanyl-tRNA synthetaseUniRule annotation
Short name:
AlaRSUniRule annotation
Gene namesi
Name:alaSUniRule annotation
Ordered Locus Names:CPn_0892, CP_0974, CpB0923
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000424: Chromosome, UP000000583: Chromosome, UP000000801: Chromosome, UP000000802: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 872872Alanine--tRNA ligasePRO_0000075087Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi115713.CPn0892.

Structurei

3D structure databases

ProteinModelPortaliQ9Z714.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156965.
KOiK01872.
OMAiWASKGSP.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z714-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSNTIRSNF LKFYANRHHT ILPSSPVFPH NDPSILFTNA GMNQFKDIFL
60 70 80 90 100
NKEKVSYSRA TTSQKCIRAG GKHNDLDNVG HTSRHLTFFE MLGNFSFGDY
110 120 130 140 150
FKAEAIAFAW EVSLSVFNFN PEGIYATVHE KDDEAFALWE AYLPTDRIFR
160 170 180 190 200
LTDKDNFWSM ANTGPCGYCS ELLFDRGPSF GNASSPLDDT DGERFLEYWN
210 220 230 240 250
LVFMEFNRTS EGSLLALPNK HVDTGAGLER LVSLIAGTHT VFEADVLREL
260 270 280 290 300
IAKTEQLSGK VYHPDDSGAA FRVIADHVRS LSFAIADGLL PGNTERGYVL
310 320 330 340 350
RKILRRSVNY GRRLGFRNPF LAEIVPSLAD AMGEAYPELK NSLSQIQKVL
360 370 380 390 400
TLEEESFFKT LDRGGNLLQQ VLKSSSSSSC ISGEDAFKLK DTYGMPIDEI
410 420 430 440 450
SLLAKDYDYS VDMDTFHKLE QEAKERSRKN VVQSQGTSES IYNELHLTSE
460 470 480 490 500
FIGYDHLSCD TFIEAIISKD HIVSSLQEKQ EGAIVLKVSP FYAEKGGQVG
510 520 530 540 550
DSGEIFCSEG TFIVTHTTSP KAGLIVHHGR ISQGSLTVEA AVTAQVNRYR
560 570 580 590 600
RKRIANNHTA CHLLHKALEI TLGDHIRQAG SYVDDTKIRL DFTHPQAISP
610 620 630 640 650
EDLLCIETLV NESIRENEPV DIREALYSDV MNSSEIKQFF GDKYSDVVRV
660 670 680 690 700
VSAGHSHELC GGTHAEATGD IGFFRITKEH AVAMGIRRIE AVTGEKAEAT
710 720 730 740 750
VHQQSEVLEE IATLLQVPRD QIVSRLTATL DERKQQDKRL NELENSLIQT
760 770 780 790 800
KLDKLIHNCH QRQGITCLVH HLAEHENHRL QQYAQCLHQR IPEKLISLWT
810 820 830 840 850
TEKNGKYIVL SRVSDDLITQ GVHAQDLLKA VLTPCGGRWG GKDQSAQGSA
860 870
PALPATEVLN ETLWQWISTQ LI
Length:872
Mass (Da):97,670
Last modified:May 1, 1999 - v1
Checksum:iDAD99C43E273CAF8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001363 Genomic DNA. Translation: AAD19030.1.
AE002161 Genomic DNA. Translation: AAF38754.1.
BA000008 Genomic DNA. Translation: BAA99100.1.
AE009440 Genomic DNA. Translation: AAP98852.1.
PIRiB86602.
H72023.
RefSeqiNP_225087.1. NC_000922.1.
NP_300949.1. NC_002491.1.
NP_445511.1. NC_002179.2.
NP_877195.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD19030; AAD19030; CPn_0892.
AAF38754; AAF38754; CP_0974.
AAP98852; AAP98852; CpB0923.
BAA99100; BAA99100; BAA99100.
GeneIDi1467602.
895680.
919656.
963442.
KEGGicpa:CP0974.
cpj:CPj0892.
cpn:CPn0892.
cpt:CpB0923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001363 Genomic DNA. Translation: AAD19030.1 .
AE002161 Genomic DNA. Translation: AAF38754.1 .
BA000008 Genomic DNA. Translation: BAA99100.1 .
AE009440 Genomic DNA. Translation: AAP98852.1 .
PIRi B86602.
H72023.
RefSeqi NP_225087.1. NC_000922.1.
NP_300949.1. NC_002491.1.
NP_445511.1. NC_002179.2.
NP_877195.1. NC_005043.1.

3D structure databases

ProteinModelPortali Q9Z714.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 115713.CPn0892.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD19030 ; AAD19030 ; CPn_0892 .
AAF38754 ; AAF38754 ; CP_0974 .
AAP98852 ; AAP98852 ; CpB0923 .
BAA99100 ; BAA99100 ; BAA99100 .
GeneIDi 1467602.
895680.
919656.
963442.
KEGGi cpa:CP0974.
cpj:CPj0892.
cpn:CPn0892.
cpt:CpB0923.

Phylogenomic databases

eggNOGi COG0013.
HOGENOMi HOG000156965.
KOi K01872.
OMAi WASKGSP.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci CPNE115711:GI7B-1005-MONOMER.
CPNE115713:GHEY-932-MONOMER.
CPNE138677:GH8N-924-MONOMER.
CPNE182082:GH4N-954-MONOMER.
BRENDAi 2.5.1.55. 1311.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiSYA_CHLPN
AccessioniPrimary (citable) accession number: Q9Z714
Secondary accession number(s): Q9JQE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3