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Q9Z714

- SYA_CHLPN

UniProt

Q9Z714 - SYA_CHLPN

Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

    Catalytic activityi

    ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi558 – 5581ZincUniRule annotation
    Metal bindingi562 – 5621ZincUniRule annotation
    Metal bindingi660 – 6601ZincUniRule annotation
    Metal bindingi664 – 6641ZincUniRule annotation

    GO - Molecular functioni

    1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-KW
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciCPNE115711:GI7B-1005-MONOMER.
    CPNE115713:GHEY-932-MONOMER.
    CPNE138677:GH8N-924-MONOMER.
    CPNE182082:GH4N-954-MONOMER.
    BRENDAi2.5.1.55. 1311.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
    Alternative name(s):
    Alanyl-tRNA synthetaseUniRule annotation
    Short name:
    AlaRSUniRule annotation
    Gene namesi
    Name:alaSUniRule annotation
    Ordered Locus Names:CPn_0892, CP_0974, CpB0923
    OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
    Taxonomic identifieri83558 [NCBI]
    Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
    ProteomesiUP000000424: Chromosome, UP000000583: Chromosome, UP000000801: Chromosome, UP000000802: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 872872Alanine--tRNA ligasePRO_0000075087Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi115713.CPn0892.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z714.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0013.
    HOGENOMiHOG000156965.
    KOiK01872.
    OMAiWASKGSP.
    OrthoDBiEOG6Q2SQ2.

    Family and domain databases

    HAMAPiMF_00036_B. Ala_tRNA_synth_B.
    InterProiIPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR003156. DHHA1_dom.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view]
    PfamiPF02272. DHHA1. 1 hit.
    PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view]
    PRINTSiPR00980. TRNASYNTHALA.
    SMARTiSM00863. tRNA_SAD. 1 hit.
    [Graphical view]
    SUPFAMiSSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsiTIGR00344. alaS. 1 hit.
    PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z714-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSNTIRSNF LKFYANRHHT ILPSSPVFPH NDPSILFTNA GMNQFKDIFL    50
    NKEKVSYSRA TTSQKCIRAG GKHNDLDNVG HTSRHLTFFE MLGNFSFGDY 100
    FKAEAIAFAW EVSLSVFNFN PEGIYATVHE KDDEAFALWE AYLPTDRIFR 150
    LTDKDNFWSM ANTGPCGYCS ELLFDRGPSF GNASSPLDDT DGERFLEYWN 200
    LVFMEFNRTS EGSLLALPNK HVDTGAGLER LVSLIAGTHT VFEADVLREL 250
    IAKTEQLSGK VYHPDDSGAA FRVIADHVRS LSFAIADGLL PGNTERGYVL 300
    RKILRRSVNY GRRLGFRNPF LAEIVPSLAD AMGEAYPELK NSLSQIQKVL 350
    TLEEESFFKT LDRGGNLLQQ VLKSSSSSSC ISGEDAFKLK DTYGMPIDEI 400
    SLLAKDYDYS VDMDTFHKLE QEAKERSRKN VVQSQGTSES IYNELHLTSE 450
    FIGYDHLSCD TFIEAIISKD HIVSSLQEKQ EGAIVLKVSP FYAEKGGQVG 500
    DSGEIFCSEG TFIVTHTTSP KAGLIVHHGR ISQGSLTVEA AVTAQVNRYR 550
    RKRIANNHTA CHLLHKALEI TLGDHIRQAG SYVDDTKIRL DFTHPQAISP 600
    EDLLCIETLV NESIRENEPV DIREALYSDV MNSSEIKQFF GDKYSDVVRV 650
    VSAGHSHELC GGTHAEATGD IGFFRITKEH AVAMGIRRIE AVTGEKAEAT 700
    VHQQSEVLEE IATLLQVPRD QIVSRLTATL DERKQQDKRL NELENSLIQT 750
    KLDKLIHNCH QRQGITCLVH HLAEHENHRL QQYAQCLHQR IPEKLISLWT 800
    TEKNGKYIVL SRVSDDLITQ GVHAQDLLKA VLTPCGGRWG GKDQSAQGSA 850
    PALPATEVLN ETLWQWISTQ LI 872
    Length:872
    Mass (Da):97,670
    Last modified:May 1, 1999 - v1
    Checksum:iDAD99C43E273CAF8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001363 Genomic DNA. Translation: AAD19030.1.
    AE002161 Genomic DNA. Translation: AAF38754.1.
    BA000008 Genomic DNA. Translation: BAA99100.1.
    AE009440 Genomic DNA. Translation: AAP98852.1.
    PIRiB86602.
    H72023.
    RefSeqiNP_225087.1. NC_000922.1.
    NP_300949.1. NC_002491.1.
    NP_445511.1. NC_002179.2.
    NP_877195.1. NC_005043.1.

    Genome annotation databases

    EnsemblBacteriaiAAD19030; AAD19030; CPn_0892.
    AAF38754; AAF38754; CP_0974.
    AAP98852; AAP98852; CpB0923.
    BAA99100; BAA99100; BAA99100.
    GeneIDi1467602.
    895680.
    919656.
    963442.
    KEGGicpa:CP0974.
    cpj:CPj0892.
    cpn:CPn0892.
    cpt:CpB0923.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001363 Genomic DNA. Translation: AAD19030.1 .
    AE002161 Genomic DNA. Translation: AAF38754.1 .
    BA000008 Genomic DNA. Translation: BAA99100.1 .
    AE009440 Genomic DNA. Translation: AAP98852.1 .
    PIRi B86602.
    H72023.
    RefSeqi NP_225087.1. NC_000922.1.
    NP_300949.1. NC_002491.1.
    NP_445511.1. NC_002179.2.
    NP_877195.1. NC_005043.1.

    3D structure databases

    ProteinModelPortali Q9Z714.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 115713.CPn0892.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD19030 ; AAD19030 ; CPn_0892 .
    AAF38754 ; AAF38754 ; CP_0974 .
    AAP98852 ; AAP98852 ; CpB0923 .
    BAA99100 ; BAA99100 ; BAA99100 .
    GeneIDi 1467602.
    895680.
    919656.
    963442.
    KEGGi cpa:CP0974.
    cpj:CPj0892.
    cpn:CPn0892.
    cpt:CpB0923.

    Phylogenomic databases

    eggNOGi COG0013.
    HOGENOMi HOG000156965.
    KOi K01872.
    OMAi WASKGSP.
    OrthoDBi EOG6Q2SQ2.

    Enzyme and pathway databases

    BioCyci CPNE115711:GI7B-1005-MONOMER.
    CPNE115713:GHEY-932-MONOMER.
    CPNE138677:GH8N-924-MONOMER.
    CPNE182082:GH4N-954-MONOMER.
    BRENDAi 2.5.1.55. 1311.

    Family and domain databases

    HAMAPi MF_00036_B. Ala_tRNA_synth_B.
    InterProi IPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR003156. DHHA1_dom.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view ]
    Pfami PF02272. DHHA1. 1 hit.
    PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view ]
    PRINTSi PR00980. TRNASYNTHALA.
    SMARTi SM00863. tRNA_SAD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsi TIGR00344. alaS. 1 hit.
    PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CWL029.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AR39.
    3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
      Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
      Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: J138.
    4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
      Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TW-183.

    Entry informationi

    Entry nameiSYA_CHLPN
    AccessioniPrimary (citable) accession number: Q9Z714
    Secondary accession number(s): Q9JQE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3