Bifunctional enzyme murC/ddl - Chlamydia pneumoniae

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Reviewed, UniProtKB/Swiss-Prot Q9Z701 (MUDD_CHLPN)

Last modified February 9, 2010. Version 90. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Bifunctional enzyme murC/ddl
Including the following 2 domains:
    1- Recommended name:
            UDP-N-acetylmuramate--L-alanine ligase
              EC=6.3.2.8
        Alternative name(s):
            UDP-N-acetylmuramoyl-L-alanine synthetase
    2- Recommended name:
            D-alanine--D-alanine ligase
              EC=6.3.2.4
        Alternative name(s):
            D-alanylalanine synthetase
            D-Ala-D-Ala ligase

Gene names

Name:	murC/ddl
Ordered Locus Names:	CPn_0905, CP_0961, CpB0937

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

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Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Cell wall formation. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046 ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00046
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	In the N-terminal section; belongs to the murCDEF family. In the C-terminal section; belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

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Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 809

809

Bifunctional enzyme murC/ddl HAMAP MF_00046

PRO_0000177915

Regions

Domain

573 – 784

212

ATP-grasp

Nucleotide binding

111 – 117

ATP Potential

Nucleotide binding

606 – 661

ATP By similarity

Region

1 – 450

450

UDP-N-acetylmuramate--alanine ligase HAMAP MF_00046

Region

451 – 809

359

D-alanine--D-alanine ligase HAMAP MF_00046

Sites

Metal binding

738

Magnesium or manganese 1 By similarity

Metal binding

751

Magnesium or manganese 1 By similarity

Metal binding

751

Magnesium or manganese 2 By similarity

Metal binding

753

Magnesium or manganese 2 By similarity

Experimental info

Sequence conflict

560

L → P in AAP98866. Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z701-1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 1A204C6B20E03B47
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FASTA

809

89,964

        10         20         30         40         50         60 
MKGTPQYHFI GIGGIGMSAL AHILLDRGYE VSGSDLYESY TIESLKAKGA RCFSGHDSSH 

        70         80         90        100        110        120 
VPHDAVVVYS SSIAPDNVEY LTAIQRSSRL LHRAELLSQL MEGYESILVS GSHGKTGTSS 

       130        140        150        160        170        180 
LIRAIFQEAQ KDPSYAIGGL AANCLNGYSG SSKIFVAEAD ESDGSLKHYT PRAVVITNID 

       190        200        210        220        230        240 
NEHLNNYAGN LDNLVQVIQD FSRKVTDLNK VFYNGDCPIL KGNVQGISYG YSPECQLHIV 

       250        260        270        280        290        300 
SYNQKAWQSH FSFTFLGQEY QDIELNLPGQ HNAANAAAAC GVALTFGIDI NIIRKALKKF 

       310        320        330        340        350        360 
SGVHRRLERK NISESFLFLE DYAHHPVEVA HTLRSVRDAV GLRRVIAIFQ PHRFSRLEEC 

       370        380        390        400        410        420 
LQTFPKAFQE ADEVILTDVY SAGESPRESI ILSDLAEQIR KSSYVHCCYV PHGDIVDYLR 

       430        440        450        460        470        480 
NYIRIHDVCV SLGAGNIYTI GEALKDFNPK KLSIGLVCGG KSCEHDISLL SAQHVSKYIS 

       490        500        510        520        530        540 
PEFYDVSYFI INRQGLWRTG KDFPHLIEET QGDSPLSSEI ASALAKVDCL FPVLHGPFGE 

       550        560        570        580        590        600 
DGTIQGFFEI LGKPYAGPSL SLAATAMDKL LTKRIASAVG VPVVPYQPLN LCFWKRNPEL 

       610        620        630        640        650        660 
CIQNLIETFS FPMIVKTAHL GSSIGIFLVR DKEELQEKIS EAFLYDTDVF VEESRLGSRE 

       670        680        690        700        710        720 
IEVSCIGHSS SWYCMAGPNE RCGASGFIDY QEKYGFDGID CAKISFDLQL SQESLDCVRE 

       730        740        750        760        770        780 
LAERVYRAMQ GKGSARIDFF LDEEGNYWLS EVNPIPGMTA ASPFLQAFVH AGWTQEQIVD 

       790        800 
HFIIDALHKF DKQQTIEQAF TKEQDLVKR

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References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD19043.1. AE002161 Genomic DNA. Translation: AAF38741.1. BA000008 Genomic DNA. Translation: BAA99113.1. AE009440 Genomic DNA. Translation: AAP98866.1.
PIR	D72022. G86603.
RefSeq	NP_225100.1. NP_300962.1. NP_445498.1. NP_877209.1.
3D structure databases
SMR	Q9Z701. Positions 6-446, 451-796.
ModBase	Search...
2-D gel databases
PHCI-2DPAGE	Q9Z701.
Genome annotation databases
GeneID	1467616. 895305. 919670. 963426.
GenomeReviews	Gene locus CPn_0905 in contig AE001363_GR. Gene locus CP_0961 in contig AE002161_GR. Gene locus CpB0937 in contig AE009440_GR. Gene locus murC/ddl in contig BA000008_GR.
KEGG	cpa:CP0961. cpn:CPn0905. cpt:CpB0937.
TIGR	CP_0961.
Phylogenomic databases
HOGENOM	HBG413852.
OMA	RRMERKN.
PhylomeDB	Q9Z701.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0961-MONOMER. CPNE115713:CPN0905-MONOMER. CPNE138677:CPJ0905-MONOMER. CPNE182082:CPB0937-MONOMER.
BRENDA	6.3.2.4. 264832. 6.3.2.8. 264832.
Family and domain databases
HAMAP	MF_00046. MurC. Fused. [Tree] MF_00047. Dala_Dala_lig. Fused. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit. TIGR01082. murC. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

MUDD_CHLPN

Accession

Primary (citable) accession number: Q9Z701
Secondary accession number(s): Q9JQB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

2-D gel databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents