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Q9Z701 (MUDD_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional enzyme MurC/Ddl

Including the following 2 domains:

  1. UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
    Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase
  2. D-alanine--D-alanine ligase
    EC=6.3.2.4
    Alternative name(s):
    D-Ala-D-Ala ligase
    D-alanylalanine synthetase
Gene names
Name:murC/ddl
Ordered Locus Names:CPn_0905, CP_0961, CpB0937
OrganismChlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]
Taxonomic identifier83558 [NCBI]
Taxonomic lineageBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia

Protein attributes

Sequence length809 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00046

Catalytic activity

ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP-Rule MF_00046

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00046

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00046

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00046.

Sequence similarities

In the N-terminal section; belongs to the MurCDEF family.

In the C-terminal section; belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 809809Bifunctional enzyme MurC/Ddl HAMAP-Rule MF_00046
PRO_0000177915

Regions

Domain573 – 784212ATP-grasp
Nucleotide binding111 – 1177ATP Potential
Nucleotide binding606 – 66156ATP By similarity
Region1 – 450450UDP-N-acetylmuramate--alanine ligase HAMAP-Rule MF_00046
Region451 – 809359D-alanine--D-alanine ligase HAMAP-Rule MF_00046

Sites

Metal binding7381Magnesium or manganese 1 By similarity
Metal binding7511Magnesium or manganese 1 By similarity
Metal binding7511Magnesium or manganese 2 By similarity
Metal binding7531Magnesium or manganese 2 By similarity

Experimental info

Sequence conflict5601L → P in AAP98866. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9Z701 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 1A204C6B20E03B47

FASTA80989,964
        10         20         30         40         50         60 
MKGTPQYHFI GIGGIGMSAL AHILLDRGYE VSGSDLYESY TIESLKAKGA RCFSGHDSSH 

        70         80         90        100        110        120 
VPHDAVVVYS SSIAPDNVEY LTAIQRSSRL LHRAELLSQL MEGYESILVS GSHGKTGTSS 

       130        140        150        160        170        180 
LIRAIFQEAQ KDPSYAIGGL AANCLNGYSG SSKIFVAEAD ESDGSLKHYT PRAVVITNID 

       190        200        210        220        230        240 
NEHLNNYAGN LDNLVQVIQD FSRKVTDLNK VFYNGDCPIL KGNVQGISYG YSPECQLHIV 

       250        260        270        280        290        300 
SYNQKAWQSH FSFTFLGQEY QDIELNLPGQ HNAANAAAAC GVALTFGIDI NIIRKALKKF 

       310        320        330        340        350        360 
SGVHRRLERK NISESFLFLE DYAHHPVEVA HTLRSVRDAV GLRRVIAIFQ PHRFSRLEEC 

       370        380        390        400        410        420 
LQTFPKAFQE ADEVILTDVY SAGESPRESI ILSDLAEQIR KSSYVHCCYV PHGDIVDYLR 

       430        440        450        460        470        480 
NYIRIHDVCV SLGAGNIYTI GEALKDFNPK KLSIGLVCGG KSCEHDISLL SAQHVSKYIS 

       490        500        510        520        530        540 
PEFYDVSYFI INRQGLWRTG KDFPHLIEET QGDSPLSSEI ASALAKVDCL FPVLHGPFGE 

       550        560        570        580        590        600 
DGTIQGFFEI LGKPYAGPSL SLAATAMDKL LTKRIASAVG VPVVPYQPLN LCFWKRNPEL 

       610        620        630        640        650        660 
CIQNLIETFS FPMIVKTAHL GSSIGIFLVR DKEELQEKIS EAFLYDTDVF VEESRLGSRE 

       670        680        690        700        710        720 
IEVSCIGHSS SWYCMAGPNE RCGASGFIDY QEKYGFDGID CAKISFDLQL SQESLDCVRE 

       730        740        750        760        770        780 
LAERVYRAMQ GKGSARIDFF LDEEGNYWLS EVNPIPGMTA ASPFLQAFVH AGWTQEQIVD 

       790        800 
HFIIDALHKF DKQQTIEQAF TKEQDLVKR 

« Hide

References

[1]"Comparative genomes of Chlamydia pneumoniae and C. trachomatis."
Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S.
Nat. Genet. 21:385-389(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CWL029.
[2]"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39."
Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. expand/collapse author list , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M.
Nucleic Acids Res. 28:1397-1406(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AR39.
[3]"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J138.
[4]"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TW-183.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001363 Genomic DNA. Translation: AAD19043.1.
AE002161 Genomic DNA. Translation: AAF38741.1.
BA000008 Genomic DNA. Translation: BAA99113.1.
AE009440 Genomic DNA. Translation: AAP98866.1.
PIRD72022.
G86603.
RefSeqNP_225100.1. NC_000922.1.
NP_300962.1. NC_002491.1.
NP_445498.1. NC_002179.2.
NP_877209.1. NC_005043.1.

3D structure databases

ProteinModelPortalQ9Z701.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING115713.CPn0905.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD19043; AAD19043; CPn_0905.
AAF38741; AAF38741; CP_0961.
AAP98866; AAP98866; CpB0937.
BAA99113; BAA99113; BAA99113.
GeneID1467616.
895305.
919670.
963426.
KEGGcpa:CP0961.
cpj:CPj0905.
cpn:CPn0905.
cpt:CpB0937.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000034913.
KOK01921.
K01924.
OMARIDFFLD.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycCPNE115711:GI7B-991-MONOMER.
CPNE115713:GHEY-946-MONOMER.
CPNE138677:GH8N-938-MONOMER.
CPNE182082:GH4N-969-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.1190.10. 1 hit.
3.40.50.20. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPMF_00046. MurC.
MF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR016185. PreATP-grasp_dom.
IPR005758. UDP-N-AcMur_Ala_ligase.
[Graphical view]
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
SSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
TIGR01082. murC. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMUDD_CHLPN
AccessionPrimary (citable) accession number: Q9Z701
Secondary accession number(s): Q9JQB5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways