Bifunctional enzyme MurC/Ddl - Chlamydia pneumoniae

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Q9Z701 (MUDD_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Bifunctional enzyme MurC/Ddl

Including the following 2 domains:

UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8
Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase
D-alanine--D-alanine ligase
EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase

Gene names

Name:	murC/ddl
Ordered Locus Names:	CPn_0905, CP_0961, CpB0937

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP-Rule MF_00046 ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00046
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00046
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the MurCDEF family. In the C-terminal section; belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 809

809

Bifunctional enzyme MurC/Ddl HAMAP-Rule MF_00046

PRO_0000177915

Regions

Domain

573 – 784

212

ATP-grasp

Nucleotide binding

111 – 117

ATP Potential

Nucleotide binding

606 – 661

ATP By similarity

Region

1 – 450

450

UDP-N-acetylmuramate--alanine ligase HAMAP-Rule MF_00046

Region

451 – 809

359

D-alanine--D-alanine ligase HAMAP-Rule MF_00046

Sites

Metal binding

738

Magnesium or manganese 1 By similarity

Metal binding

751

Magnesium or manganese 1 By similarity

Metal binding

751

Magnesium or manganese 2 By similarity

Metal binding

753

Magnesium or manganese 2 By similarity

Experimental info

Sequence conflict

560

L → P in AAP98866. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z701 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 1A204C6B20E03B47
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FASTA

809

89,964

        10         20         30         40         50         60 
MKGTPQYHFI GIGGIGMSAL AHILLDRGYE VSGSDLYESY TIESLKAKGA RCFSGHDSSH 

        70         80         90        100        110        120 
VPHDAVVVYS SSIAPDNVEY LTAIQRSSRL LHRAELLSQL MEGYESILVS GSHGKTGTSS 

       130        140        150        160        170        180 
LIRAIFQEAQ KDPSYAIGGL AANCLNGYSG SSKIFVAEAD ESDGSLKHYT PRAVVITNID 

       190        200        210        220        230        240 
NEHLNNYAGN LDNLVQVIQD FSRKVTDLNK VFYNGDCPIL KGNVQGISYG YSPECQLHIV 

       250        260        270        280        290        300 
SYNQKAWQSH FSFTFLGQEY QDIELNLPGQ HNAANAAAAC GVALTFGIDI NIIRKALKKF 

       310        320        330        340        350        360 
SGVHRRLERK NISESFLFLE DYAHHPVEVA HTLRSVRDAV GLRRVIAIFQ PHRFSRLEEC 

       370        380        390        400        410        420 
LQTFPKAFQE ADEVILTDVY SAGESPRESI ILSDLAEQIR KSSYVHCCYV PHGDIVDYLR 

       430        440        450        460        470        480 
NYIRIHDVCV SLGAGNIYTI GEALKDFNPK KLSIGLVCGG KSCEHDISLL SAQHVSKYIS 

       490        500        510        520        530        540 
PEFYDVSYFI INRQGLWRTG KDFPHLIEET QGDSPLSSEI ASALAKVDCL FPVLHGPFGE 

       550        560        570        580        590        600 
DGTIQGFFEI LGKPYAGPSL SLAATAMDKL LTKRIASAVG VPVVPYQPLN LCFWKRNPEL 

       610        620        630        640        650        660 
CIQNLIETFS FPMIVKTAHL GSSIGIFLVR DKEELQEKIS EAFLYDTDVF VEESRLGSRE 

       670        680        690        700        710        720 
IEVSCIGHSS SWYCMAGPNE RCGASGFIDY QEKYGFDGID CAKISFDLQL SQESLDCVRE 

       730        740        750        760        770        780 
LAERVYRAMQ GKGSARIDFF LDEEGNYWLS EVNPIPGMTA ASPFLQAFVH AGWTQEQIVD 

       790        800 
HFIIDALHKF DKQQTIEQAF TKEQDLVKR

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References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD19043.1. AE002161 Genomic DNA. Translation: AAF38741.1. BA000008 Genomic DNA. Translation: BAA99113.1. AE009440 Genomic DNA. Translation: AAP98866.1.
PIR	D72022. G86603.
RefSeq	NP_225100.1. NC_000922.1. NP_300962.1. NC_002491.1. NP_445498.1. NC_002179.2. NP_877209.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z701.
ModBase	Search...
Protein-protein interaction databases
STRING	115713.CPn0905.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD19043; AAD19043; CPn_0905. AAF38741; AAF38741; CP_0961. AAP98866; AAP98866; CpB0937. BAA99113; BAA99113; BAA99113.
GeneID	1467616. 895305. 919670. 963426.
KEGG	cpa:CP0961. cpj:CPj0905. cpn:CPn0905. cpt:CpB0937.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1181.
HOGENOM	HOG000034913.
KO	K01921. K01924.
OMA	REIEVSC.
ProtClustDB	PRK14573.
Enzyme and pathway databases
BioCyc	CPNE115711:GI7B-959-MONOMER. CPNE115713:GHEY-906-MONOMER. CPNE138677:GH8N-895-MONOMER. CPNE182082:GH4N-934-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.1190.10. 1 hit. 3.40.50.20. 1 hit. 3.40.50.720. 1 hit. 3.90.190.20. 1 hit.
HAMAP	MF_00046. MurC. Fused. MF_00047. Dala_Dala_lig. Fused.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR016185. PreATP-grasp_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit. SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit. TIGR01082. murC. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MUDD_CHLPN

Accession

Primary (citable) accession number: Q9Z701
Secondary accession number(s): Q9JQB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents