Reviewed, UniProtKB/Swiss-Prot Q9Z6Y8 (IPYR_CHLPN)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	CPn_0918, CP_0948, CpB0950

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137491
Sites
	Metal binding	92	1	Magnesium 1 By similarity
	Metal binding	97	1	Magnesium 1 By similarity
	Metal binding	97	1	Magnesium 2 By similarity
	Metal binding	130	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z6Y8-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 4ADD075D2680F361	FASTA	215	23,981
	10 20 30 40 50 60 MSKKPLYVAH PWHSPTLTQD NYESLCCYIE ITPYDSVKFE LDKATGLLKV DRPQKFSNFC 70 80 90 100 110 120 PCLYGLLPQT YCGTASGNYS GEQTRREGIQ GDKDPLDVCV LTEKNIHHGN ILLQARPIGG 130 140 150 160 170 180 LRIIDSGEAD DKIIAVLEDD LVFAEIEDIS DCPGTVLDMI QHYFLTYKAT PNHLIKGSPA 190 200 210 KIEIVGIYGK KEAQKVIQLA HEDYLSYIGD TAEVN

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001363 Genomic DNA. Translation: AAD19056.1. AE002161 Genomic DNA. Translation: AAF38730.1. BA000008 Genomic DNA. Translation: BAA99126.1. AE009440 Genomic DNA. Translation: AAP98879.1.
PIR	D86605. E72020.
RefSeq	NP_225113.1. NP_300975.1. NP_445485.1. NP_877222.1.
3D structure databases
HSSP	HSSP built from PDB template 1QEZ based on UniProtKB P50308.
ModBase	Search...
2-D gel databases
PHCI-2DPAGE	Q9Z6Y8.
Genome annotation databases
GeneID	1467629. 895310. 919683. 963413.
GenomeReviews	Gene locus CPn_0918 in contig AE001363_GR. Gene locus CP_0948 in contig AE002161_GR. Gene locus CpB0950 in contig AE009440_GR. Gene locus ppa in contig BA000008_GR.
KEGG	cpa:CP0948. cpn:CPn0918. cpt:CpB0950.
TIGR	CP_0948.
Phylogenomic databases
HOGENOM	Q9Z6Y8.
OMA	NYESLCC.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0948-MON. CPNE115713:CPN0918-MON. CPNE138677:CPJ0918-MON. CPNE182082:CPB0950-MON.
BRENDA	3.6.1.1. 264832.
Family and domain databases
HAMAP	MF_00209. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
ProDom	PD002014. Inorg_pphsph. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00387. PPASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_CHLPN

Accession

Primary (citable) accession number: Q9Z6Y8
Secondary accession number(s): Q9JQ89

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)