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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.By similarity

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.

Pathway:ilipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-924-MONOMER.
CPNE115713:GHEY-1007-MONOMER.
CPNE138677:GH8N-999-MONOMER.
CPNE182082:GH4N-1035-MONOMER.
UniPathwayiUPA00359; UER00481.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthase (EC:2.4.1.182)
Gene namesi
Name:lpxB
Ordered Locus Names:CPn_0965, CP_0895, CpB1002
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000583 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 604604Lipid-A-disaccharide synthasePRO_0000190160Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi115713.CPn0965.

Structurei

3D structure databases

ProteinModelPortaliQ9Z6U3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 220220UnknownAdd
BLAST
Regioni221 – 604384Lipid-A-disaccharide synthaseAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the LpxB family.Curated

Phylogenomic databases

eggNOGiCOG3952.
HOGENOMiHOG000034725.
KOiK00748.
OMAiKIIHYVC.
OrthoDBiEOG6FBWZR.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
IPR011499. Lipid_A_biosynth.
[Graphical view]
PfamiPF07578. LAB_N. 2 hits.
PF02684. LpxB. 1 hit.
[Graphical view]
ProDomiPD339292. LAB_N. 2 hits.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z6U3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPSGLVYLL YPLGFLASLF FGSAFSIQWW LSKKRKEVYA PRSFWILSSI
60 70 80 90 100
GATLMIVHGT IQSQFPVTVL HVINLIIYLR NLNITSSRPI SFRATLVLMA
110 120 130 140 150
LSVVFVTLPF LYVNMEWMAS PNIFHLPLPP AQLSWHLIGC LGLAIFSGRF
160 170 180 190 200
LIQWFYIESN NTKDFPLLFW KIGLLGGLLA LVYFIRIGDP INILCYGCGL
210 220 230 240 250
FPSIANLRLF YKEQRSTPYL DTHCFLSAGE ASGDILGGKL IQSIKSLYPN
260 270 280 290 300
IRFWGVGGPA MRQEGLQPIL NMEEFQVSGF AEVLGSLFRL YRNYRKILKT
310 320 330 340 350
ILKHKPATLI FIDFPDFHLL LIKKLRKHGY RGKIIHYVCP SIWAWRPKRK
360 370 380 390 400
RILEQHLDML LLILPFEEGL FKNTSLETVY LGHPLVEEIS DYKEQASWKE
410 420 430 440 450
KFLNSDRPIV AAFPGSRRGD ISRNLRIQVQ AFLNSSLSQT HQFVVSSSSA
460 470 480 490 500
KYDEIIEDTL KAEGCQHSQI IPMNFRYELM RSCDCALAKC GTIVLETALN
510 520 530 540 550
QTPTIVMCRL RPFDTFLAKY IFKILLPAYS LPNIIMNSVI FPEFIGGKKD
560 570 580 590 600
FHPEEIATAL DLLNQHGSKE KQKEDCRKLC KVMTTGQIAS EEFLKRIFDT

LPAV
Length:604
Mass (Da):69,014
Last modified:May 1, 1999 - v1
Checksum:i094BF1B7013E07C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19101.1.
AE002161 Genomic DNA. Translation: AAF38682.1.
BA000008 Genomic DNA. Translation: BAA99173.1.
AE009440 Genomic DNA. Translation: AAP98931.1.
PIRiB72014.
C86611.
RefSeqiNP_225158.1. NC_000922.1.

Genome annotation databases

EnsemblBacteriaiAAD19101; AAD19101; CPn_0965.
AAF38682; AAF38682; CP_0895.
AAP98931; AAP98931; CpB1002.
BAA99173; BAA99173; BAA99173.
GeneIDi895627.
KEGGicpa:CP0895.
cpj:CPj0965.
cpn:CPn0965.
cpt:CpB1002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19101.1.
AE002161 Genomic DNA. Translation: AAF38682.1.
BA000008 Genomic DNA. Translation: BAA99173.1.
AE009440 Genomic DNA. Translation: AAP98931.1.
PIRiB72014.
C86611.
RefSeqiNP_225158.1. NC_000922.1.

3D structure databases

ProteinModelPortaliQ9Z6U3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi115713.CPn0965.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD19101; AAD19101; CPn_0965.
AAF38682; AAF38682; CP_0895.
AAP98931; AAP98931; CpB1002.
BAA99173; BAA99173; BAA99173.
GeneIDi895627.
KEGGicpa:CP0895.
cpj:CPj0965.
cpn:CPn0965.
cpt:CpB1002.

Phylogenomic databases

eggNOGiCOG3952.
HOGENOMiHOG000034725.
KOiK00748.
OMAiKIIHYVC.
OrthoDBiEOG6FBWZR.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.
BioCyciCPNE115711:GI7B-924-MONOMER.
CPNE115713:GHEY-1007-MONOMER.
CPNE138677:GH8N-999-MONOMER.
CPNE182082:GH4N-1035-MONOMER.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
IPR011499. Lipid_A_biosynth.
[Graphical view]
PfamiPF07578. LAB_N. 2 hits.
PF02684. LpxB. 1 hit.
[Graphical view]
ProDomiPD339292. LAB_N. 2 hits.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR00215. lpxB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiLPXB_CHLPN
AccessioniPrimary (citable) accession number: Q9Z6U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.