Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9Z6S5

- RIR1_CHLPN

UniProt

Q9Z6S5 - RIR1_CHLPN

Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Enzyme regulationi

    Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei440 – 4401SubstrateBy similarity
    Sitei456 – 4561Important for hydrogen atom transferBy similarity
    Sitei463 – 4631Allosteric effector bindingBy similarity
    Binding sitei484 – 4841Substrate; via amide nitrogenBy similarity
    Sitei493 – 4931Allosteric effector bindingBy similarity
    Active sitei668 – 6681Proton acceptorBy similarity
    Active sitei670 – 6701Cysteine radical intermediateBy similarity
    Active sitei672 – 6721Proton acceptorBy similarity
    Sitei685 – 6851Important for hydrogen atom transferBy similarity
    Sitei988 – 9881Important for electron transferBy similarity
    Sitei989 – 9891Important for electron transferBy similarity
    Sitei1040 – 10401Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei1043 – 10431Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCPNE115711:GI7B-901-MONOMER.
    CPNE115713:GHEY-1026-MONOMER.
    CPNE138677:GH8N-1016-MONOMER.
    CPNE182082:GH4N-1054-MONOMER.
    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase
    Gene namesi
    Name:nrdA
    Ordered Locus Names:CPn_0984, CP_0872, CpB1021
    OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
    Taxonomic identifieri83558 [NCBI]
    Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
    ProteomesiUP000000424: Chromosome, UP000000583: Chromosome, UP000000801: Chromosome, UP000000802: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10441044Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187211Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi456 ↔ 685Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Protein-protein interaction databases

    STRINGi115713.CPn0984.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z6S5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 111103ATP-cone 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini118 – 217100ATP-cone 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini235 – 32591ATP-cone 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni455 – 4562Substrate bindingBy similarity
    Regioni668 – 6725Substrate bindingBy similarity
    Regioni855 – 8595Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 3 ATP-cone domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0209.
    HOGENOMiHOG000057036.
    KOiK00525.
    OMAiEACLMCQ.
    OrthoDBiEOG6J48HC.

    Family and domain databases

    InterProiIPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view]
    PfamiPF03477. ATP-cone. 3 hits.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    SUPFAMiSSF48168. SSF48168. 1 hit.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS51161. ATP_CONE. 3 hits.
    PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z6S5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEVEEKHYT IVKRNGMFVP FNQDRIFQAL EAAFRDTRSL ETSSPLPKDL     50
    EESIAQITHK VVKEVLAKIS EGQVVTVERI QDLVESQLYI SGLQDVARDY 100
    IVYRDQRKAE RGNSSSIIAI IRRDGGSAKF NPMKISAALE KAFRATLQIN 150
    GMTPPATLSE INDLTLRIVE DVLSLHGEEA INLEEIQDIV EKQLMVAGYY 200
    DVAKNYILYR EARARARANK DQDGQEEFVP QEETYVVQKE DGTTYLLRKT 250
    DLEKRFSWAC KRFPKTTDSQ LLADMAFMNL YSGIKEDEVT TACIMAARAN 300
    IEREPDYAFI AAELLTSSLY EETLGCSSQD PNLSEIHKKH FKEYILNGEE 350
    YRLNPQLKDY DLDALSEVLD LSRDQQFSYM GVQNLYDRYF NLHEGRRLET 400
    AQIFWMRVSM GLALNEGEQK NFWAITFYNL LSTFRYTPAT PTLFNSGMRH 450
    SQLSSCYLST VKDDLSHIYK VISDNALLSK WAGGIGNDWT DVRATGAVIK 500
    GTNGKSQGVI PFIKVANDTA IAVNQGGKRK GAMCVYLENW HLDYEDFLEL 550
    RKNTGDERRR THDINTASWI PDLFFKRLEK KGMWTLFSPD DVPGLHEAYG 600
    LEFEKLYEEY ERKVESGEIR LYKKVEAEVL WRKMLSMLYE TGHPWITFKD 650
    PSNIRSNQDH VGVVRCSNLC TEILLNCSES ETAVCNLGSI NLVEHIRNDK 700
    LDEEKLKETI SIAIRILDNV IDLNFYPTPE AKQANLTHRA VGLGVMGFQD 750
    VLYELNISYA SQEAVEFSDE CSEIIAYYAI LASSLLAKER GTYASYSGSK 800
    WDRGYLPLDT IELLKETRGE HNVLVDTSSK KDWTPVRDTI QKYGMRNSQV 850
    MAIAPTATIS NIIGVTQSIE PMYKHLFVKS NLSGEFTIPN TYLIKKLKEL 900
    GLWDAEMLDD LKYFDGSLLE IERIPNHLKK LFLTAFEIEP EWIIECTSRR 950
    QKWIDMGVSL NLYLAEPDGK KLSNMYLTAW KKGLKTTYYL RSQAATSVEK 1000
    SFIDINKRGI QPRWMKNKSA STSIVVERKT TPVCSMEEGC ESCQ 1044
    Length:1,044
    Mass (Da):119,399
    Last modified:May 1, 1999 - v1
    Checksum:i0A7379FA01B7372E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001363 Genomic DNA. Translation: AAD19121.1.
    AE002161 Genomic DNA. Translation: AAF38661.1.
    BA000008 Genomic DNA. Translation: BAA99191.1.
    AE009440 Genomic DNA. Translation: AAP98950.1.
    PIRiA72010.
    E86613.
    RefSeqiNP_225178.1. NC_000922.1.
    NP_301039.1. NC_002491.1.
    NP_445410.1. NC_002179.2.
    NP_877293.1. NC_005043.1.

    Genome annotation databases

    EnsemblBacteriaiAAD19121; AAD19121; CPn_0984.
    AAF38661; AAF38661; CP_0872.
    AAP98950; AAP98950; CpB1021.
    BAA99191; BAA99191; BAA99191.
    GeneIDi1467700.
    895606.
    919750.
    963324.
    KEGGicpa:CP0872.
    cpj:CPj0984.
    cpn:CPn0984.
    cpt:CpB1021.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001363 Genomic DNA. Translation: AAD19121.1 .
    AE002161 Genomic DNA. Translation: AAF38661.1 .
    BA000008 Genomic DNA. Translation: BAA99191.1 .
    AE009440 Genomic DNA. Translation: AAP98950.1 .
    PIRi A72010.
    E86613.
    RefSeqi NP_225178.1. NC_000922.1.
    NP_301039.1. NC_002491.1.
    NP_445410.1. NC_002179.2.
    NP_877293.1. NC_005043.1.

    3D structure databases

    ProteinModelPortali Q9Z6S5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 115713.CPn0984.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD19121 ; AAD19121 ; CPn_0984 .
    AAF38661 ; AAF38661 ; CP_0872 .
    AAP98950 ; AAP98950 ; CpB1021 .
    BAA99191 ; BAA99191 ; BAA99191 .
    GeneIDi 1467700.
    895606.
    919750.
    963324.
    KEGGi cpa:CP0872.
    cpj:CPj0984.
    cpn:CPn0984.
    cpt:CpB1021.

    Phylogenomic databases

    eggNOGi COG0209.
    HOGENOMi HOG000057036.
    KOi K00525.
    OMAi EACLMCQ.
    OrthoDBi EOG6J48HC.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .
    BioCyci CPNE115711:GI7B-901-MONOMER.
    CPNE115713:GHEY-1026-MONOMER.
    CPNE138677:GH8N-1016-MONOMER.
    CPNE182082:GH4N-1054-MONOMER.

    Family and domain databases

    InterProi IPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view ]
    Pfami PF03477. ATP-cone. 3 hits.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    SUPFAMi SSF48168. SSF48168. 1 hit.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS51161. ATP_CONE. 3 hits.
    PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CWL029.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AR39.
    3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
      Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
      Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: J138.
    4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
      Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TW-183.

    Entry informationi

    Entry nameiRIR1_CHLPN
    AccessioniPrimary (citable) accession number: Q9Z6S5
    Secondary accession number(s): Q9JQC5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3