SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9Z6S5

- RIR1_CHLPN

UniProt

Q9Z6S5 - RIR1_CHLPN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Ribonucleoside-diphosphate reductase subunit alpha
Gene
nrdA, CPn_0984, CP_0872, CpB1021
Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei440 – 4401Substrate By similarity
Sitei456 – 4561Important for hydrogen atom transfer By similarity
Sitei463 – 4631Allosteric effector binding By similarity
Binding sitei484 – 4841Substrate; via amide nitrogen By similarity
Sitei493 – 4931Allosteric effector binding By similarity
Active sitei668 – 6681Proton acceptor By similarity
Active sitei670 – 6701Cysteine radical intermediate By similarity
Active sitei672 – 6721Proton acceptor By similarity
Sitei685 – 6851Important for hydrogen atom transfer By similarity
Sitei988 – 9881Important for electron transfer By similarity
Sitei989 – 9891Important for electron transfer By similarity
Sitei1040 – 10401Interacts with thioredoxin/glutaredoxin By similarity
Sitei1043 – 10431Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-901-MONOMER.
CPNE115713:GHEY-1026-MONOMER.
CPNE138677:GH8N-1016-MONOMER.
CPNE182082:GH4N-1054-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:CPn_0984, CP_0872, CpB1021
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000424: Chromosome, UP000000583: Chromosome, UP000000801: Chromosome, UP000000802: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi456 ↔ 685Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi115713.CPn0984.

Structurei

3D structure databases

ProteinModelPortaliQ9Z6S5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 111103ATP-cone 1
Add
BLAST
Domaini118 – 217100ATP-cone 2
Add
BLAST
Domaini235 – 32591ATP-cone 3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni455 – 4562Substrate binding By similarity
Regioni668 – 6725Substrate binding By similarity
Regioni855 – 8595Substrate binding By similarity

Sequence similaritiesi

Contains 3 ATP-cone domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000057036.
KOiK00525.
OMAiEACLMCQ.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 3 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z6S5-1 [UniParc]FASTAAdd to Basket

« Hide

MVEVEEKHYT IVKRNGMFVP FNQDRIFQAL EAAFRDTRSL ETSSPLPKDL     50
EESIAQITHK VVKEVLAKIS EGQVVTVERI QDLVESQLYI SGLQDVARDY 100
IVYRDQRKAE RGNSSSIIAI IRRDGGSAKF NPMKISAALE KAFRATLQIN 150
GMTPPATLSE INDLTLRIVE DVLSLHGEEA INLEEIQDIV EKQLMVAGYY 200
DVAKNYILYR EARARARANK DQDGQEEFVP QEETYVVQKE DGTTYLLRKT 250
DLEKRFSWAC KRFPKTTDSQ LLADMAFMNL YSGIKEDEVT TACIMAARAN 300
IEREPDYAFI AAELLTSSLY EETLGCSSQD PNLSEIHKKH FKEYILNGEE 350
YRLNPQLKDY DLDALSEVLD LSRDQQFSYM GVQNLYDRYF NLHEGRRLET 400
AQIFWMRVSM GLALNEGEQK NFWAITFYNL LSTFRYTPAT PTLFNSGMRH 450
SQLSSCYLST VKDDLSHIYK VISDNALLSK WAGGIGNDWT DVRATGAVIK 500
GTNGKSQGVI PFIKVANDTA IAVNQGGKRK GAMCVYLENW HLDYEDFLEL 550
RKNTGDERRR THDINTASWI PDLFFKRLEK KGMWTLFSPD DVPGLHEAYG 600
LEFEKLYEEY ERKVESGEIR LYKKVEAEVL WRKMLSMLYE TGHPWITFKD 650
PSNIRSNQDH VGVVRCSNLC TEILLNCSES ETAVCNLGSI NLVEHIRNDK 700
LDEEKLKETI SIAIRILDNV IDLNFYPTPE AKQANLTHRA VGLGVMGFQD 750
VLYELNISYA SQEAVEFSDE CSEIIAYYAI LASSLLAKER GTYASYSGSK 800
WDRGYLPLDT IELLKETRGE HNVLVDTSSK KDWTPVRDTI QKYGMRNSQV 850
MAIAPTATIS NIIGVTQSIE PMYKHLFVKS NLSGEFTIPN TYLIKKLKEL 900
GLWDAEMLDD LKYFDGSLLE IERIPNHLKK LFLTAFEIEP EWIIECTSRR 950
QKWIDMGVSL NLYLAEPDGK KLSNMYLTAW KKGLKTTYYL RSQAATSVEK 1000
SFIDINKRGI QPRWMKNKSA STSIVVERKT TPVCSMEEGC ESCQ 1044
Length:1,044
Mass (Da):119,399
Last modified:May 1, 1999 - v1
Checksum:i0A7379FA01B7372E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001363 Genomic DNA. Translation: AAD19121.1.
AE002161 Genomic DNA. Translation: AAF38661.1.
BA000008 Genomic DNA. Translation: BAA99191.1.
AE009440 Genomic DNA. Translation: AAP98950.1.
PIRiA72010.
E86613.
RefSeqiNP_225178.1. NC_000922.1.
NP_301039.1. NC_002491.1.
NP_445410.1. NC_002179.2.
NP_877293.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD19121; AAD19121; CPn_0984.
AAF38661; AAF38661; CP_0872.
AAP98950; AAP98950; CpB1021.
BAA99191; BAA99191; BAA99191.
GeneIDi1467700.
895606.
919750.
963324.
KEGGicpa:CP0872.
cpj:CPj0984.
cpn:CPn0984.
cpt:CpB1021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001363 Genomic DNA. Translation: AAD19121.1 .
AE002161 Genomic DNA. Translation: AAF38661.1 .
BA000008 Genomic DNA. Translation: BAA99191.1 .
AE009440 Genomic DNA. Translation: AAP98950.1 .
PIRi A72010.
E86613.
RefSeqi NP_225178.1. NC_000922.1.
NP_301039.1. NC_002491.1.
NP_445410.1. NC_002179.2.
NP_877293.1. NC_005043.1.

3D structure databases

ProteinModelPortali Q9Z6S5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 115713.CPn0984.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD19121 ; AAD19121 ; CPn_0984 .
AAF38661 ; AAF38661 ; CP_0872 .
AAP98950 ; AAP98950 ; CpB1021 .
BAA99191 ; BAA99191 ; BAA99191 .
GeneIDi 1467700.
895606.
919750.
963324.
KEGGi cpa:CP0872.
cpj:CPj0984.
cpn:CPn0984.
cpt:CpB1021.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000057036.
KOi K00525.
OMAi EACLMCQ.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci CPNE115711:GI7B-901-MONOMER.
CPNE115713:GHEY-1026-MONOMER.
CPNE138677:GH8N-1016-MONOMER.
CPNE182082:GH4N-1054-MONOMER.

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 3 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiRIR1_CHLPN
AccessioniPrimary (citable) accession number: Q9Z6S5
Secondary accession number(s): Q9JQC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi