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Q9Z6S5

- RIR1_CHLPN

UniProt

Q9Z6S5 - RIR1_CHLPN

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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei440 – 4401SubstrateBy similarity
Sitei456 – 4561Important for hydrogen atom transferBy similarity
Sitei463 – 4631Allosteric effector bindingBy similarity
Binding sitei484 – 4841Substrate; via amide nitrogenBy similarity
Sitei493 – 4931Allosteric effector bindingBy similarity
Active sitei668 – 6681Proton acceptorBy similarity
Active sitei670 – 6701Cysteine radical intermediateBy similarity
Active sitei672 – 6721Proton acceptorBy similarity
Sitei685 – 6851Important for hydrogen atom transferBy similarity
Sitei988 – 9881Important for electron transferBy similarity
Sitei989 – 9891Important for electron transferBy similarity
Sitei1040 – 10401Interacts with thioredoxin/glutaredoxinBy similarity
Sitei1043 – 10431Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-901-MONOMER.
CPNE115713:GHEY-1026-MONOMER.
CPNE138677:GH8N-1016-MONOMER.
CPNE182082:GH4N-1054-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:CPn_0984, CP_0872, CpB1021
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000424: Chromosome, UP000000583: Chromosome, UP000000801: Chromosome, UP000000802: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Ribonucleoside-diphosphate reductase subunit alphaPRO_0000187211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi456 ↔ 685Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi115713.CPn0984.

Structurei

3D structure databases

ProteinModelPortaliQ9Z6S5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 111103ATP-cone 1PROSITE-ProRule annotationAdd
BLAST
Domaini118 – 217100ATP-cone 2PROSITE-ProRule annotationAdd
BLAST
Domaini235 – 32591ATP-cone 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni455 – 4562Substrate bindingBy similarity
Regioni668 – 6725Substrate bindingBy similarity
Regioni855 – 8595Substrate bindingBy similarity

Sequence similaritiesi

Contains 3 ATP-cone domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000057036.
KOiK00525.
OMAiEACLMCQ.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 3 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z6S5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVEVEEKHYT IVKRNGMFVP FNQDRIFQAL EAAFRDTRSL ETSSPLPKDL
60 70 80 90 100
EESIAQITHK VVKEVLAKIS EGQVVTVERI QDLVESQLYI SGLQDVARDY
110 120 130 140 150
IVYRDQRKAE RGNSSSIIAI IRRDGGSAKF NPMKISAALE KAFRATLQIN
160 170 180 190 200
GMTPPATLSE INDLTLRIVE DVLSLHGEEA INLEEIQDIV EKQLMVAGYY
210 220 230 240 250
DVAKNYILYR EARARARANK DQDGQEEFVP QEETYVVQKE DGTTYLLRKT
260 270 280 290 300
DLEKRFSWAC KRFPKTTDSQ LLADMAFMNL YSGIKEDEVT TACIMAARAN
310 320 330 340 350
IEREPDYAFI AAELLTSSLY EETLGCSSQD PNLSEIHKKH FKEYILNGEE
360 370 380 390 400
YRLNPQLKDY DLDALSEVLD LSRDQQFSYM GVQNLYDRYF NLHEGRRLET
410 420 430 440 450
AQIFWMRVSM GLALNEGEQK NFWAITFYNL LSTFRYTPAT PTLFNSGMRH
460 470 480 490 500
SQLSSCYLST VKDDLSHIYK VISDNALLSK WAGGIGNDWT DVRATGAVIK
510 520 530 540 550
GTNGKSQGVI PFIKVANDTA IAVNQGGKRK GAMCVYLENW HLDYEDFLEL
560 570 580 590 600
RKNTGDERRR THDINTASWI PDLFFKRLEK KGMWTLFSPD DVPGLHEAYG
610 620 630 640 650
LEFEKLYEEY ERKVESGEIR LYKKVEAEVL WRKMLSMLYE TGHPWITFKD
660 670 680 690 700
PSNIRSNQDH VGVVRCSNLC TEILLNCSES ETAVCNLGSI NLVEHIRNDK
710 720 730 740 750
LDEEKLKETI SIAIRILDNV IDLNFYPTPE AKQANLTHRA VGLGVMGFQD
760 770 780 790 800
VLYELNISYA SQEAVEFSDE CSEIIAYYAI LASSLLAKER GTYASYSGSK
810 820 830 840 850
WDRGYLPLDT IELLKETRGE HNVLVDTSSK KDWTPVRDTI QKYGMRNSQV
860 870 880 890 900
MAIAPTATIS NIIGVTQSIE PMYKHLFVKS NLSGEFTIPN TYLIKKLKEL
910 920 930 940 950
GLWDAEMLDD LKYFDGSLLE IERIPNHLKK LFLTAFEIEP EWIIECTSRR
960 970 980 990 1000
QKWIDMGVSL NLYLAEPDGK KLSNMYLTAW KKGLKTTYYL RSQAATSVEK
1010 1020 1030 1040
SFIDINKRGI QPRWMKNKSA STSIVVERKT TPVCSMEEGC ESCQ
Length:1,044
Mass (Da):119,399
Last modified:May 1, 1999 - v1
Checksum:i0A7379FA01B7372E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19121.1.
AE002161 Genomic DNA. Translation: AAF38661.1.
BA000008 Genomic DNA. Translation: BAA99191.1.
AE009440 Genomic DNA. Translation: AAP98950.1.
PIRiA72010.
E86613.
RefSeqiNP_225178.1. NC_000922.1.
NP_301039.1. NC_002491.1.
NP_445410.1. NC_002179.2.
NP_877293.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD19121; AAD19121; CPn_0984.
AAF38661; AAF38661; CP_0872.
AAP98950; AAP98950; CpB1021.
BAA99191; BAA99191; BAA99191.
GeneIDi1467700.
895606.
919750.
963324.
KEGGicpa:CP0872.
cpj:CPj0984.
cpn:CPn0984.
cpt:CpB1021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19121.1 .
AE002161 Genomic DNA. Translation: AAF38661.1 .
BA000008 Genomic DNA. Translation: BAA99191.1 .
AE009440 Genomic DNA. Translation: AAP98950.1 .
PIRi A72010.
E86613.
RefSeqi NP_225178.1. NC_000922.1.
NP_301039.1. NC_002491.1.
NP_445410.1. NC_002179.2.
NP_877293.1. NC_005043.1.

3D structure databases

ProteinModelPortali Q9Z6S5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 115713.CPn0984.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD19121 ; AAD19121 ; CPn_0984 .
AAF38661 ; AAF38661 ; CP_0872 .
AAP98950 ; AAP98950 ; CpB1021 .
BAA99191 ; BAA99191 ; BAA99191 .
GeneIDi 1467700.
895606.
919750.
963324.
KEGGi cpa:CP0872.
cpj:CPj0984.
cpn:CPn0984.
cpt:CpB1021.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000057036.
KOi K00525.
OMAi EACLMCQ.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .
BioCyci CPNE115711:GI7B-901-MONOMER.
CPNE115713:GHEY-1026-MONOMER.
CPNE138677:GH8N-1016-MONOMER.
CPNE182082:GH4N-1054-MONOMER.

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF03477. ATP-cone. 3 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.

Entry informationi

Entry nameiRIR1_CHLPN
AccessioniPrimary (citable) accession number: Q9Z6S5
Secondary accession number(s): Q9JQC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3