Reviewed, UniProtKB/Swiss-Prot Q9Z6S5 (RIR1_CHLPN)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase

Gene names

Name:	nrdA
Ordered Locus Names:	CPn_0984, CP_0872, CpB1021

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	1044 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family. Contains 3 ATP-cone domains.

Ontologies

Keywords
Biological process	DNA replication
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1044	1044	Ribonucleoside-diphosphate reductase subunit alpha		PRO_0000187211
Regions
	Domain	9 – 111	103	ATP-cone 1
	Domain	118 – 217	100	ATP-cone 2
	Domain	235 – 325	91	ATP-cone 3
Sites
	Active site	456	1	Hydrogen atom transfer By similarity
	Active site	668	1	Proton acceptor By similarity
	Active site	670	1	Proton acceptor By similarity
	Active site	672	1	Proton acceptor By similarity
	Active site	685	1	Hydrogen atom transfer By similarity
	Active site	988	1	Electron transfer By similarity
	Active site	989	1	Electron transfer By similarity
	Site	463	1	Allosteric effector binding By similarity
	Site	493	1	Allosteric effector binding By similarity
	Site	1040	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	1043	1	Interacts with thioredoxin/glutaredoxin By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z6S5-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 0A7379FA01B7372E	FASTA	1,044	119,399
	10 20 30 40 50 60 MVEVEEKHYT IVKRNGMFVP FNQDRIFQAL EAAFRDTRSL ETSSPLPKDL EESIAQITHK 70 80 90 100 110 120 VVKEVLAKIS EGQVVTVERI QDLVESQLYI SGLQDVARDY IVYRDQRKAE RGNSSSIIAI 130 140 150 160 170 180 IRRDGGSAKF NPMKISAALE KAFRATLQIN GMTPPATLSE INDLTLRIVE DVLSLHGEEA 190 200 210 220 230 240 INLEEIQDIV EKQLMVAGYY DVAKNYILYR EARARARANK DQDGQEEFVP QEETYVVQKE 250 260 270 280 290 300 DGTTYLLRKT DLEKRFSWAC KRFPKTTDSQ LLADMAFMNL YSGIKEDEVT TACIMAARAN 310 320 330 340 350 360 IEREPDYAFI AAELLTSSLY EETLGCSSQD PNLSEIHKKH FKEYILNGEE YRLNPQLKDY 370 380 390 400 410 420 DLDALSEVLD LSRDQQFSYM GVQNLYDRYF NLHEGRRLET AQIFWMRVSM GLALNEGEQK 430 440 450 460 470 480 NFWAITFYNL LSTFRYTPAT PTLFNSGMRH SQLSSCYLST VKDDLSHIYK VISDNALLSK 490 500 510 520 530 540 WAGGIGNDWT DVRATGAVIK GTNGKSQGVI PFIKVANDTA IAVNQGGKRK GAMCVYLENW 550 560 570 580 590 600 HLDYEDFLEL RKNTGDERRR THDINTASWI PDLFFKRLEK KGMWTLFSPD DVPGLHEAYG 610 620 630 640 650 660 LEFEKLYEEY ERKVESGEIR LYKKVEAEVL WRKMLSMLYE TGHPWITFKD PSNIRSNQDH 670 680 690 700 710 720 VGVVRCSNLC TEILLNCSES ETAVCNLGSI NLVEHIRNDK LDEEKLKETI SIAIRILDNV 730 740 750 760 770 780 IDLNFYPTPE AKQANLTHRA VGLGVMGFQD VLYELNISYA SQEAVEFSDE CSEIIAYYAI 790 800 810 820 830 840 LASSLLAKER GTYASYSGSK WDRGYLPLDT IELLKETRGE HNVLVDTSSK KDWTPVRDTI 850 860 870 880 890 900 QKYGMRNSQV MAIAPTATIS NIIGVTQSIE PMYKHLFVKS NLSGEFTIPN TYLIKKLKEL 910 920 930 940 950 960 GLWDAEMLDD LKYFDGSLLE IERIPNHLKK LFLTAFEIEP EWIIECTSRR QKWIDMGVSL 970 980 990 1000 1010 1020 NLYLAEPDGK KLSNMYLTAW KKGLKTTYYL RSQAATSVEK SFIDINKRGI QPRWMKNKSA 1030 1040 STSIVVERKT TPVCSMEEGC ESCQ

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001363 Genomic DNA. Translation: AAD19121.1. AE002161 Genomic DNA. Translation: AAF38661.1. BA000008 Genomic DNA. Translation: BAA99191.1. AE017160 Genomic DNA. Translation: AAP98950.1.
PIR	A72010. E86613.
RefSeq	NP_225178.1. NP_301039.1. NP_445410.1. NP_877293.1.
3D structure databases
HSSP	HSSP built from PDB template 5R1R based on UniProtKB P00452.
ModBase	Search...
Genome annotation databases
GeneID	1467700. 895606. 919750. 963324.
GenomeReviews	Gene locus CPn_0984 in contig AE001363_GR. Gene locus CP_0872 in contig AE002161_GR. Gene locus CpB1021 in contig AE009440_GR. Gene locus nrdA in contig BA000008_GR.
KEGG	cpa:CP0872. cpn:CPn0984. cpt:CpB1021.
TIGR	CP_0872.
Phylogenomic databases
HOGENOM	Q9Z6S5.
OMA	Q9Z6S5. ATLFYPL.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0872-MON. CPNE115713:CPN0984-MON. CPNE138677:CPJ0984-MON. CPNE182082:CPB1021-MON.
BRENDA	1.17.4.1. 264832.
Family and domain databases
InterPro	IPR005144. ATP-cone. IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF03477. ATP-cone. 3 hits. PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS51161. ATP_CONE. 3 hits. PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_CHLPN

Accession

Primary (citable) accession number: Q9Z6S5
Secondary accession number(s): Q9JQC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)