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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei440SubstrateBy similarity1
Sitei456Important for hydrogen atom transferBy similarity1
Sitei463Allosteric effector bindingBy similarity1
Binding sitei484Substrate; via amide nitrogenBy similarity1
Sitei493Allosteric effector bindingBy similarity1
Active sitei668Proton acceptorBy similarity1
Active sitei670Cysteine radical intermediateBy similarity1
Active sitei672Proton acceptorBy similarity1
Sitei685Important for hydrogen atom transferBy similarity1
Sitei988Important for electron transferBy similarity1
Sitei989Important for electron transferBy similarity1
Sitei1040Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei1043Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:CPn_0984, CP_0872, CpB1021
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000583 Componenti: Chromosome
  • UP000000801 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872111 – 1044Ribonucleoside-diphosphate reductase subunit alphaAdd BLAST1044

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi456 ↔ 685Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ9Z6S5.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi115713.CPn0984.

Structurei

3D structure databases

ProteinModelPortaliQ9Z6S5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 111ATP-cone 1PROSITE-ProRule annotationAdd BLAST103
Domaini118 – 217ATP-cone 2PROSITE-ProRule annotationAdd BLAST100
Domaini235 – 325ATP-cone 3PROSITE-ProRule annotationAdd BLAST91

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni455 – 456Substrate bindingBy similarity2
Regioni668 – 672Substrate bindingBy similarity5
Regioni855 – 859Substrate bindingBy similarity5

Sequence similaritiesi

Contains 3 ATP-cone domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
HOGENOMiHOG000057036.
KOiK00525.
OMAiFDYLGLQ.
OrthoDBiPOG091H03NG.

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 3 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z6S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEVEEKHYT IVKRNGMFVP FNQDRIFQAL EAAFRDTRSL ETSSPLPKDL
60 70 80 90 100
EESIAQITHK VVKEVLAKIS EGQVVTVERI QDLVESQLYI SGLQDVARDY
110 120 130 140 150
IVYRDQRKAE RGNSSSIIAI IRRDGGSAKF NPMKISAALE KAFRATLQIN
160 170 180 190 200
GMTPPATLSE INDLTLRIVE DVLSLHGEEA INLEEIQDIV EKQLMVAGYY
210 220 230 240 250
DVAKNYILYR EARARARANK DQDGQEEFVP QEETYVVQKE DGTTYLLRKT
260 270 280 290 300
DLEKRFSWAC KRFPKTTDSQ LLADMAFMNL YSGIKEDEVT TACIMAARAN
310 320 330 340 350
IEREPDYAFI AAELLTSSLY EETLGCSSQD PNLSEIHKKH FKEYILNGEE
360 370 380 390 400
YRLNPQLKDY DLDALSEVLD LSRDQQFSYM GVQNLYDRYF NLHEGRRLET
410 420 430 440 450
AQIFWMRVSM GLALNEGEQK NFWAITFYNL LSTFRYTPAT PTLFNSGMRH
460 470 480 490 500
SQLSSCYLST VKDDLSHIYK VISDNALLSK WAGGIGNDWT DVRATGAVIK
510 520 530 540 550
GTNGKSQGVI PFIKVANDTA IAVNQGGKRK GAMCVYLENW HLDYEDFLEL
560 570 580 590 600
RKNTGDERRR THDINTASWI PDLFFKRLEK KGMWTLFSPD DVPGLHEAYG
610 620 630 640 650
LEFEKLYEEY ERKVESGEIR LYKKVEAEVL WRKMLSMLYE TGHPWITFKD
660 670 680 690 700
PSNIRSNQDH VGVVRCSNLC TEILLNCSES ETAVCNLGSI NLVEHIRNDK
710 720 730 740 750
LDEEKLKETI SIAIRILDNV IDLNFYPTPE AKQANLTHRA VGLGVMGFQD
760 770 780 790 800
VLYELNISYA SQEAVEFSDE CSEIIAYYAI LASSLLAKER GTYASYSGSK
810 820 830 840 850
WDRGYLPLDT IELLKETRGE HNVLVDTSSK KDWTPVRDTI QKYGMRNSQV
860 870 880 890 900
MAIAPTATIS NIIGVTQSIE PMYKHLFVKS NLSGEFTIPN TYLIKKLKEL
910 920 930 940 950
GLWDAEMLDD LKYFDGSLLE IERIPNHLKK LFLTAFEIEP EWIIECTSRR
960 970 980 990 1000
QKWIDMGVSL NLYLAEPDGK KLSNMYLTAW KKGLKTTYYL RSQAATSVEK
1010 1020 1030 1040
SFIDINKRGI QPRWMKNKSA STSIVVERKT TPVCSMEEGC ESCQ
Length:1,044
Mass (Da):119,399
Last modified:May 1, 1999 - v1
Checksum:i0A7379FA01B7372E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19121.1.
AE002161 Genomic DNA. Translation: AAF38661.1.
BA000008 Genomic DNA. Translation: BAA99191.1.
AE009440 Genomic DNA. Translation: AAP98950.1.
PIRiA72010.
E86613.
RefSeqiNP_225178.1. NC_000922.1.
WP_010883617.1. NZ_LN847257.1.

Genome annotation databases

EnsemblBacteriaiAAD19121; AAD19121; CPn_0984.
AAF38661; AAF38661; CP_0872.
AAP98950; AAP98950; CpB1021.
BAA99191; BAA99191; BAA99191.
GeneIDi895606.
KEGGicpa:CP_0872.
cpj:nrdA.
cpn:CPn0984.
cpt:CpB1021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19121.1.
AE002161 Genomic DNA. Translation: AAF38661.1.
BA000008 Genomic DNA. Translation: BAA99191.1.
AE009440 Genomic DNA. Translation: AAP98950.1.
PIRiA72010.
E86613.
RefSeqiNP_225178.1. NC_000922.1.
WP_010883617.1. NZ_LN847257.1.

3D structure databases

ProteinModelPortaliQ9Z6S5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi115713.CPn0984.

Proteomic databases

PRIDEiQ9Z6S5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD19121; AAD19121; CPn_0984.
AAF38661; AAF38661; CP_0872.
AAP98950; AAP98950; CpB1021.
BAA99191; BAA99191; BAA99191.
GeneIDi895606.
KEGGicpa:CP_0872.
cpj:nrdA.
cpn:CPn0984.
cpt:CpB1021.

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
HOGENOMiHOG000057036.
KOiK00525.
OMAiFDYLGLQ.
OrthoDBiPOG091H03NG.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 3 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_CHLPN
AccessioniPrimary (citable) accession number: Q9Z6S5
Secondary accession number(s): Q9JQC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.