Q9Z6Q0 (AMPM_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18

Alternative name(s):
Peptidase M

Gene names

Name:	map
Ordered Locus Names:	CPn_1009, CP_0844, CpB1046

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.
Sequence caution	The sequence AAF38634.1 differs from that shown. Reason: Erroneous initiation. The sequence AAP98976.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Methionine aminopeptidase		PRO_0000148933
Sites
	Metal binding	135	1	Cobalt 1 By similarity
	Metal binding	146	1	Cobalt 1 By similarity
	Metal binding	146	1	Cobalt 2 By similarity
	Metal binding	209	1	Cobalt 2 By similarity
	Metal binding	241	1	Cobalt 2 By similarity
	Metal binding	273	1	Cobalt 1 By similarity
	Metal binding	273	1	Cobalt 2 By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	216	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z6Q0 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 094F6D6E17F23DE9	FASTA	291	32,596
	10 20 30 40 50 60 MKRNDPCWCG SGRKWKQCHY PQPPKMSPEA LKQHYASQYN ILLKTPEQKA KIYNACQITA 70 80 90 100 110 120 RILDELCKAS QKGVTTNELD ELSQELHKKY DAIAAPFHYG SPPFPKTICT SLNEVICHGI 130 140 150 160 170 180 PNDIPLKDGD IMNIDVSCIV DGYYGDCSRM VMIGEVPEIK KKICQAALEC LNDSIAILKP 190 200 210 220 230 240 GIPLCEIGEA IEARADTYGF SVVDQFVGHG VGIEFHENPY VPHYRNRSMI PLAPGMIFTI 250 260 270 280 290 EPMINVGKKE GVVDPKNQWE ARTCDNQPSA QWEHTIAITE TGYEILTLLN D

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD19146.1. AE002161 Genomic DNA. Translation: AAF38634.1. Different initiation. BA000008 Genomic DNA. Translation: BAA99216.1. AE009440 Genomic DNA. Translation: AAP98976.1. Different initiation.
PIR	A81531. E72008. F86616.
RefSeq	NP_225203.1. NC_000922.1. NP_301064.1. NC_002491.1. NP_445382.2. NC_002179.2. NP_877319.2. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z6Q0.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1467726. 894643. 919776. 963127.
GenomeReviews	Gene locus CPn_1009 in contig AE001363_GR. Gene locus CP_0844 in contig AE002161_GR. Gene locus CpB1046 in contig AE009440_GR. Gene locus map in contig BA000008_GR.
KEGG	cpa:CP0844. cpn:CPn1009. cpt:CpB1046.
TIGR	CP_0844.
Phylogenomic databases
HOGENOM	HBG299384.
OMA	FHENPYV.
ProtClustDB	PRK12318.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0844-MONOMER. CPNE115713:CPN1009-MONOMER. CPNE138677:CPJ1009-MONOMER. CPNE182082:CPB1046-MONOMER.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. IPR004027. SEC_C_motif. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
KO	K01265.
Pfam	PF00557. Peptidase_M24. 1 hit. PF02810. SEC-C. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_CHLPN

Accession

Primary (citable) accession number: Q9Z6Q0
Secondary accession number(s): Q9JQD4, Q9K1X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program