Q9Z6P6 (FUMC_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	CPn_1013, CP_0840, CpB1051

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161268
Regions
	Region	126 – 129	4	B site By similarity
	Region	136 – 138	3	Substrate binding By similarity
Sites
	Binding site	97	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z6P6 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: A59DA2D41F2100F1	FASTA	460	50,441
	10 20 30 40 50 60 MRQEKDSLGI VEVPEDKLYG AQTMRSRNFF SWGPELMPYE VIRALVWIKK CAAQANQDLG 70 80 90 100 110 120 FLDSKHCDMI VAAADEILEG GFEEHFPLKV WQTGSGTQSN MNVNEVIANL AIRHHGGVLG 130 140 150 160 170 180 SKDPIHPNDH VNKSQSSNDV FPTAMHIAAV ISLKNKLIPA LDHMIRVLDA KVEEFRHDVK 190 200 210 220 230 240 IGRTHLMDAV PMTLGQEFSG YSSQLRHCLE SIAFSLAHLY ELAIGATAVG TGLNVPEGFV 250 260 270 280 290 300 EKIIHYLRKE TDEPFIPASN YFSALSCHDA LVDAHGSLAT LACALTKIAT DLSFLGSGPR 310 320 330 340 350 360 CGLGELFFPE NEPGSSIMPG KVNPTQCEAL QMVCAQVLGN NQTVIIGGSR GNFELNVMKP 370 380 390 400 410 420 VIIYNFLQSV DLLSEGMRAF SEFFVKGLKV NKARLQDNIN NSLMLVTALA PVLGYDKCSK 430 440 450 460 AALKAFHESI SLKEACLALG YLSEKEFDRL VVPENMVGNH

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD19150.1. AE002161 Genomic DNA. Translation: AAF38632.1. BA000008 Genomic DNA. Translation: BAA99220.1. AE009440 Genomic DNA. Translation: AAP98980.1.
PIR	A72009. B86617.
RefSeq	NP_225207.1. NC_000922.1. NP_301068.1. NC_002491.1. NP_445379.1. NC_002179.2. NP_877323.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z6P6.
SMR	Q9Z6P6. Positions 1-456.
ModBase	Search...
2D gel databases
PHCI-2DPAGE	Q9Z6P6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1467730. 894645. 919783. 963045.
GenomeReviews	Gene locus CPn_1013 in contig AE001363_GR. Gene locus CP_0840 in contig AE002161_GR. Gene locus CpB1051 in contig AE009440_GR. Gene locus fumC in contig BA000008_GR.
KEGG	cpa:CP0840. cpn:CPn1013. cpt:CpB1051.
TIGR	CP_0840.
Phylogenomic databases
HOGENOM	HBG284369.
OMA	RIEKDTM.
ProtClustDB	PRK00485.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0840-MONOMER. CPNE115713:CPN1013-MONOMER. CPNE138677:CPJ1013-MONOMER. CPNE182082:CPB1051-MONOMER.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01679.
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00979. FumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_CHLPN

Accession

Primary (citable) accession number: Q9Z6P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program