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Q9Z6M7

- AAXB_CHLPN

UniProt

Q9Z6M7 - AAXB_CHLPN

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Protein

Pyruvoyl-dependent arginine decarboxylase AaxB

Gene
aaxB, CPn_1032, CP_0820, CPj1032, CpB1072
Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Part of the AaxABC system, catalyzes the decarboxylation of L-arginine. The arginine uptake by the bacterium in the macrophage may be a virulence factor against the host innate immune response.1 Publication

Catalytic activityi

L-arginine = agmatine + CO2.

Cofactori

Pyruvoyl group.

Enzyme regulationi

Inhibited by argininamide.

Kineticsi

  1. KM=5.0 mM for L-arginine1 Publication

pH dependencei

Optimum pH is 3.4.

Temperature dependencei

Thermostable. Retains 48% activity at 50 degrees Celsius, and 13% activity at 100 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei52 – 532Cleavage (non-hydrolytic)

GO - Molecular functioni

  1. arginine decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. arginine catabolic process Source: InterPro
  2. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Virulence

Keywords - Ligandi

Pyruvate

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-846-MONOMER.
CPNE115713:GHEY-1077-MONOMER.
CPNE138677:GH8N-1067-MONOMER.
CPNE182082:GH4N-1108-MONOMER.
SABIO-RKQ9Z6M7.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvoyl-dependent arginine decarboxylase AaxB (EC:4.1.1.19)
Short name:
PvlArgDC
Alternative name(s):
Biodegradative arginine decarboxylase
Cleaved into the following 2 chains:
Gene namesi
Name:aaxB
Ordered Locus Names:CPn_1032, CP_0820, CPj1032, CpB1072
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000424: Chromosome, UP000000583: Chromosome, UP000000801: Chromosome, UP000000802: Chromosome

Subcellular locationi

Cytoplasm Inferred

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi52 – 521T → S: 70% loss of activity. Significantly impaired in ability to self-cleave. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5252Pyruvoyl-dependent arginine decarboxylase subunit betaPRO_0000364033Add
BLAST
Chaini53 – 195143Pyruvoyl-dependent arginine decarboxylase subunit alphaPRO_0000364034Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531Pyruvic acid (Ser)

Interactioni

Subunit structurei

Trimer of an alpha-beta dimer.

Protein-protein interaction databases

STRINGi115713.CPn1032.

Structurei

3D structure databases

ProteinModelPortaliQ9Z6M7.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1945.
HOGENOMiHOG000025218.
OMAiWAVEYVE.
OrthoDBiEOG6Q5NT3.

Family and domain databases

Gene3Di3.50.20.10. 1 hit.
InterProiIPR016104. Pyr-dep_his/arg-deCO2ase.
IPR016105. Pyr-dep_his/arg-deCO2ase_sand.
IPR002724. Pyruvoyl-dep_arg_deCO2ase.
[Graphical view]
PfamiPF01862. PvlArgDC. 1 hit.
[Graphical view]
ProDomiPD010449. Pyruvoyl-dep_arg_deCO2ase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56271. SSF56271. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z6M7-1 [UniParc]FASTAAdd to Basket

« Hide

MAYGTRYPTL AFHTGGIGES DDGMPPQPFE TFCYDSALLQ AKIENFNIVP    50
YTSVLPKELF GNIVPVDTCV KSFKHGAVLE VIMAGRGAAL SDGTHAIATG 100
IGICWGKDKN GELIGGWAAE YVEFFPTWIN DEIAETHAKM WLKKSLQHEL 150
DLRSIAKHSE FQFFHNYINI KQKFGFCLTA LGFLNFENAE PAKVN 195
Length:195
Mass (Da):21,655
Last modified:May 1, 1999 - v1
Checksum:i1506883FB4854DA2
GO

Mass spectrometryi

Molecular mass is 24283 Da from positions 1 - 195. Determined by MALDI. 1 Publication
Molecular mass is 15875 Da from positions 53 - 195. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001363 Genomic DNA. Translation: AAD19169.1.
AE002161 Genomic DNA. Translation: AAF38615.1.
BA000008 Genomic DNA. Translation: BAA99239.1.
AE009440 Genomic DNA. Translation: AAP99001.1.
PIRiB72006.
E86619.
RefSeqiNP_225226.1. NC_000922.1.
NP_301087.1. NC_002491.1.
NP_445359.1. NC_002179.2.
NP_877344.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD19169; AAD19169; CPn_1032.
AAF38615; AAF38615; CP_0820.
AAP99001; AAP99001; CpB1072.
BAA99239; BAA99239; BAA99239.
GeneIDi1467751.
895497.
919806.
962776.
KEGGicpa:CP0820.
cpj:CPj1032.
cpn:CPn1032.
cpt:CpB1072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001363 Genomic DNA. Translation: AAD19169.1 .
AE002161 Genomic DNA. Translation: AAF38615.1 .
BA000008 Genomic DNA. Translation: BAA99239.1 .
AE009440 Genomic DNA. Translation: AAP99001.1 .
PIRi B72006.
E86619.
RefSeqi NP_225226.1. NC_000922.1.
NP_301087.1. NC_002491.1.
NP_445359.1. NC_002179.2.
NP_877344.1. NC_005043.1.

3D structure databases

ProteinModelPortali Q9Z6M7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 115713.CPn1032.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD19169 ; AAD19169 ; CPn_1032 .
AAF38615 ; AAF38615 ; CP_0820 .
AAP99001 ; AAP99001 ; CpB1072 .
BAA99239 ; BAA99239 ; BAA99239 .
GeneIDi 1467751.
895497.
919806.
962776.
KEGGi cpa:CP0820.
cpj:CPj1032.
cpn:CPn1032.
cpt:CpB1072.

Phylogenomic databases

eggNOGi COG1945.
HOGENOMi HOG000025218.
OMAi WAVEYVE.
OrthoDBi EOG6Q5NT3.

Enzyme and pathway databases

BioCyci CPNE115711:GI7B-846-MONOMER.
CPNE115713:GHEY-1077-MONOMER.
CPNE138677:GH8N-1067-MONOMER.
CPNE182082:GH4N-1108-MONOMER.
SABIO-RK Q9Z6M7.

Family and domain databases

Gene3Di 3.50.20.10. 1 hit.
InterProi IPR016104. Pyr-dep_his/arg-deCO2ase.
IPR016105. Pyr-dep_his/arg-deCO2ase_sand.
IPR002724. Pyruvoyl-dep_arg_deCO2ase.
[Graphical view ]
Pfami PF01862. PvlArgDC. 1 hit.
[Graphical view ]
ProDomi PD010449. Pyruvoyl-dep_arg_deCO2ase. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF56271. SSF56271. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.
  5. "Characterization of an acid-dependent arginine decarboxylase enzyme from Chlamydophila pneumoniae."
    Giles T.N., Graham D.E.
    J. Bacteriol. 189:7376-7383(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY, MUTAGENESIS OF THR-52.
    Strain: Kajaani 6.
  6. "Outer and inner membrane proteins compose an arginine-agmatine exchange system in Chlamydophila pneumoniae."
    Smith C.B., Graham D.E.
    J. Bacteriol. 190:7431-7440(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE.
    Strain: Kajaani 6.

Entry informationi

Entry nameiAAXB_CHLPN
AccessioniPrimary (citable) accession number: Q9Z6M7
Secondary accession number(s): Q7AHX1, Q7BWL3, Q7DE85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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