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Protein

Pyruvoyl-dependent arginine decarboxylase AaxB

Gene

aaxB

Organism
Chlamydia pneumoniae (Chlamydophila pneumoniae)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the AaxABC system, catalyzes the decarboxylation of L-arginine. The arginine uptake by the bacterium in the macrophage may be a virulence factor against the host innate immune response.1 Publication

Catalytic activityi

L-arginine = agmatine + CO2.

Cofactori

pyruvateNote: Binds 1 pyruvoyl group covalently per subunit.

Enzyme regulationi

Inhibited by argininamide.

Kineticsi

  1. KM=5.0 mM for L-arginine1 Publication

pH dependencei

Optimum pH is 3.4.1 Publication

Temperature dependencei

Thermostable. Retains 48% activity at 50 degrees Celsius, and 13% activity at 100 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei52 – 532Cleavage (non-hydrolytic)

GO - Molecular functioni

  1. arginine decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. arginine catabolic process Source: InterPro
  2. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Virulence

Keywords - Ligandi

Pyruvate

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-846-MONOMER.
CPNE115713:GHEY-1077-MONOMER.
CPNE138677:GH8N-1067-MONOMER.
CPNE182082:GH4N-1108-MONOMER.
SABIO-RKQ9Z6M7.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvoyl-dependent arginine decarboxylase AaxB (EC:4.1.1.19)
Short name:
PvlArgDC
Alternative name(s):
Biodegradative arginine decarboxylase
Cleaved into the following 2 chains:
Gene namesi
Name:aaxB
Ordered Locus Names:CPn_1032, CP_0820, CPj1032, CpB1072
OrganismiChlamydia pneumoniae (Chlamydophila pneumoniae)
Taxonomic identifieri83558 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000802 Componenti: Chromosome UP000000583 Componenti: Chromosome UP000000801 Componenti: Chromosome UP000000424 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi52 – 521T → S: 70% loss of activity. Significantly impaired in ability to self-cleave. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5252Pyruvoyl-dependent arginine decarboxylase subunit betaPRO_0000364033Add
BLAST
Chaini53 – 195143Pyruvoyl-dependent arginine decarboxylase subunit alphaPRO_0000364034Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531Pyruvic acid (Ser)

Interactioni

Subunit structurei

Trimer of an alpha-beta dimer.

Protein-protein interaction databases

STRINGi115713.CPn1032.

Structurei

3D structure databases

ProteinModelPortaliQ9Z6M7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1945.
HOGENOMiHOG000025218.
OMAiWAVEYVE.
OrthoDBiEOG6Q5NT3.

Family and domain databases

Gene3Di3.50.20.10. 1 hit.
InterProiIPR016104. Pyr-dep_his/arg-deCO2ase.
IPR016105. Pyr-dep_his/arg-deCO2ase_sand.
IPR002724. Pyruvoyl-dep_arg_deCO2ase.
[Graphical view]
PfamiPF01862. PvlArgDC. 1 hit.
[Graphical view]
ProDomiPD010449. Pyruvoyl-dep_arg_deCO2ase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56271. SSF56271. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z6M7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYGTRYPTL AFHTGGIGES DDGMPPQPFE TFCYDSALLQ AKIENFNIVP
60 70 80 90 100
YTSVLPKELF GNIVPVDTCV KSFKHGAVLE VIMAGRGAAL SDGTHAIATG
110 120 130 140 150
IGICWGKDKN GELIGGWAAE YVEFFPTWIN DEIAETHAKM WLKKSLQHEL
160 170 180 190
DLRSIAKHSE FQFFHNYINI KQKFGFCLTA LGFLNFENAE PAKVN
Length:195
Mass (Da):21,655
Last modified:May 1, 1999 - v1
Checksum:i1506883FB4854DA2
GO

Mass spectrometryi

Molecular mass is 24283 Da from positions 1 - 195. Determined by MALDI. 1 Publication
Molecular mass is 15875 Da from positions 53 - 195. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19169.1.
AE002161 Genomic DNA. Translation: AAF38615.1.
BA000008 Genomic DNA. Translation: BAA99239.1.
AE009440 Genomic DNA. Translation: AAP99001.1.
PIRiB72006.
E86619.
RefSeqiNP_225226.1. NC_000922.1.
NP_301087.1. NC_002491.1.
NP_445359.1. NC_002179.2.
NP_877344.1. NC_005043.1.

Genome annotation databases

EnsemblBacteriaiAAD19169; AAD19169; CPn_1032.
AAF38615; AAF38615; CP_0820.
AAP99001; AAP99001; CpB1072.
BAA99239; BAA99239; BAA99239.
GeneIDi895497.
962776.
KEGGicpa:CP0820.
cpj:CPj1032.
cpn:CPn1032.
cpt:CpB1072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001363 Genomic DNA. Translation: AAD19169.1.
AE002161 Genomic DNA. Translation: AAF38615.1.
BA000008 Genomic DNA. Translation: BAA99239.1.
AE009440 Genomic DNA. Translation: AAP99001.1.
PIRiB72006.
E86619.
RefSeqiNP_225226.1. NC_000922.1.
NP_301087.1. NC_002491.1.
NP_445359.1. NC_002179.2.
NP_877344.1. NC_005043.1.

3D structure databases

ProteinModelPortaliQ9Z6M7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi115713.CPn1032.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD19169; AAD19169; CPn_1032.
AAF38615; AAF38615; CP_0820.
AAP99001; AAP99001; CpB1072.
BAA99239; BAA99239; BAA99239.
GeneIDi895497.
962776.
KEGGicpa:CP0820.
cpj:CPj1032.
cpn:CPn1032.
cpt:CpB1072.

Phylogenomic databases

eggNOGiCOG1945.
HOGENOMiHOG000025218.
OMAiWAVEYVE.
OrthoDBiEOG6Q5NT3.

Enzyme and pathway databases

BioCyciCPNE115711:GI7B-846-MONOMER.
CPNE115713:GHEY-1077-MONOMER.
CPNE138677:GH8N-1067-MONOMER.
CPNE182082:GH4N-1108-MONOMER.
SABIO-RKQ9Z6M7.

Family and domain databases

Gene3Di3.50.20.10. 1 hit.
InterProiIPR016104. Pyr-dep_his/arg-deCO2ase.
IPR016105. Pyr-dep_his/arg-deCO2ase_sand.
IPR002724. Pyruvoyl-dep_arg_deCO2ase.
[Graphical view]
PfamiPF01862. PvlArgDC. 1 hit.
[Graphical view]
ProDomiPD010449. Pyruvoyl-dep_arg_deCO2ase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56271. SSF56271. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CWL029.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AR39.
  3. "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA."
    Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.
    Nucleic Acids Res. 28:2311-2314(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: J138.
  4. "The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis."
    Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TW-183.
  5. "Characterization of an acid-dependent arginine decarboxylase enzyme from Chlamydophila pneumoniae."
    Giles T.N., Graham D.E.
    J. Bacteriol. 189:7376-7383(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY, MUTAGENESIS OF THR-52.
    Strain: Kajaani 6.
  6. "Outer and inner membrane proteins compose an arginine-agmatine exchange system in Chlamydophila pneumoniae."
    Smith C.B., Graham D.E.
    J. Bacteriol. 190:7431-7440(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE.
    Strain: Kajaani 6.

Entry informationi

Entry nameiAAXB_CHLPN
AccessioniPrimary (citable) accession number: Q9Z6M7
Secondary accession number(s): Q7AHX1, Q7BWL3, Q7DE85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 1, 1999
Last modified: April 1, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.